Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Exp/5.20 Mutagen Concentration Experiment
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: For all mutagen concentrations, -4 and -6 dilutions were plated with x6 top agar. Specific procedures used were similar to that in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 Phage Selection Method Test]] | : For all mutagen concentrations, -4 and -6 dilutions were plated with x6 top agar. Specific procedures used were similar to that in [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 Phage Selection Method Test]] | ||
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+ | 5) Preliminary selection test - third round (6.3) | ||
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+ | : We plated -4 dilution titers with x6 top agar. Used similar procedure to the previous selection test. | ||
'''V) Results''' | '''V) Results''' | ||
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2) Spot test | 2) Spot test | ||
- | : Lower concentration of mutagen seen to stimulate phage production, while higher concentration inhibits | + | : Lower concentration of mutagen seen to stimulate phage production, while higher concentration inhibits phage production |
[[File:MutagenPlate1.JPG|400px|center]] | [[File:MutagenPlate1.JPG|400px|center]] | ||
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[[File:MutagenPlate3.JPG|400px|center]] | [[File:MutagenPlate3.JPG|400px|center]] | ||
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+ | 4) Preliminary selection test 2 | ||
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+ | : -6 at x6 only showed a couple plaques on each plate. For -4 at x6, there were many plaques. It seems like the number of plaques increase as we increase the mutagen concentration. | ||
Revision as of 22:19, 3 June 2013
Small Phage May - June Notebook: Experiments
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5.20 Mutagen Concentration Experiment
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Applying the mutagen (5.20)
2) Spot test (5.22)
3) Preliminary selection test (5.24)
4) Preliminary selection test - second round (5.29)
5) Preliminary selection test - third round (6.3)
V) Results 1) Applying mutagen
2) Spot test
3) Preliminary selection test
4) Preliminary selection test 2
VI) Conclusion ADD
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