Team:BYU Provo/Notebook/Cholera - Detection/Fallexp

From 2013.igem.org

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: '''Cholera Detection July Notebook'''</font>
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: '''Cholera Detection September - October Notebook'''</font>
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<font size="5" font face="Calibri"> '''July 1 - July 7''' </font>
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<font size="5" font face="Calibri"> '''September 1 - September 15''' </font>
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<font size="3" font face="Calibri"> We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage were also made. </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Fallexp/Period1/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period1/Explist|Experiment Listing]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period1/PR| Progress Report]] </font>
 
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| <font size="5" font face="Calibri"> ''July 8 - July 14'' </font>
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| <font size="5" font face="Calibri"> '''September 16 - September 30''' </font>
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<font size="3" font face="Calibri"> We did further work on purifying lambda. Also, we have been working on purifying and mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs. </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Fallexp/Period2/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/Explist|Experiment Listing]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period2/PR| Progress Report]] </font>
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period3/Dailylog|Daily log]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period3/Explist|Experiment Listing]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period3/PR| Progress Report]] </font>
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/Dailylog|Daily log]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period4/Explist|Experiment Listing]]
 
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<font size="3" font face="Calibri"> We did further work on purifying lambda. Also, we have been working on purifying and mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs. </font>
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[[Team:BYU_Provo/Notebook/CholeraDetection/Fallexp/Period3/Dailylog|Daily log]]
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Explist|Experiment Listing]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/PR| Progress Report]] </font>
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Dailylog|Daily log]]
 
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[[Team:BYU_Provo/Notebook/CholeraDetection/Springexp/Period5/Explist|Experiment Listing]]
 
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Latest revision as of 21:37, 28 October 2013


Cholera Detection September - October Notebook



Cholera Detection
March-April
May-June
July-August
September-October

September 1 - September 15


We discovered that lambda is rendered ineffective after electrporation. The children's book for our outreach project was completed. We also streaked cholera next to our lambda containing e. coli and discovered that cholera induces the lysis of our lambda phage. Our first attempts at purifying lambda phage were also made.


Daily log



Byuk.jpg


September 16 - September 30


We did further work on purifying lambda. Also, we have been working on purifying and mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs.


Daily log



BYUCDFall2.JPG
October 1 - October 28


We did further work on purifying lambda. Also, we have been working on purifying and mutating a membrane protein called SdiA. It recognizes cholera's AHL. We will use it to enable our construct to only respond to cholera's AHLs instead of all AHLs.


Daily log



BYUjello.jpg