Team:UNITN-Trento/Notebook/Labposts/06/35

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(Difference between revisions)

Latest revision as of 07:49, 3 October 2013

{ "date" : "2013-06-18", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters (part3)!!", "content" : " from the previous inoculum ( with NEB10b cells) we could only take K823000 ( duplicate) and K143012 (triplicate). Then we performed the minipreps and quantified the yields:K823000 a = 30.7 ng/ulK823000 b = 34.2 ng/ulK143012a = 26.8 ng/ulK143012b = 30.3 ng/ulK143012c = 29.5 ng/ulAs far as NEB5a are concerned, from the inoculum we made our cells competent.In the afternoon we transformed these cells with our promoters.", "tags" : "Pveg-PliaG-PlepA" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-06-19",
+	"date" : "2013-06-18",
 	"author" : "fabio-bruno",
-	"title" : "bacillus subtilis promoters and screening (part 4)!!",
+	"title" : "bacillus subtilis promoters (part3)!!",
-	"content" : "<html>  today we performed the screening protocol in order to verify the real presence of the promoters in the miniprep ( K823000a, K143012b) . To do that, we digested 600 ng of both templates with Ecor1-HF and Pst1-HF</html>",
+	"content" : "<html> from the previous inoculum ( with NEB10b cells) we could only take K823000 ( duplicate) and K143012 (triplicate). Then we performed the minipreps and quantified the yields:K823000 a = 30.7 ng/ulK823000 b = 34.2 ng/ulK143012a = 26.8 ng/ulK143012b = 30.3 ng/ulK143012c = 29.5 ng/ulAs far as NEB5a are concerned, from the inoculum we made our cells competent.In the afternoon we transformed these cells with our promoters.</html>",
 	"tags" : "Pveg-PliaG-PlepA"
 }