Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period9/Dailylog

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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="4"> '''7/17/13''' </font>
<font size="4"> '''7/17/13''' </font>
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Reviewed results of the titer from last class.
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Ran CsCl gradient for T4 group.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.17 T4 CsCl Gradient |7.17 T4 CsCl Gradient]]
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<font size="4"> '''7/19/13''' </font>
<font size="4"> '''7/19/13''' </font>
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Prepared phage for an EM.
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Discussed our results from 7.17 with the large phage group. we will be taking TEM pictures of the potentially mutated T4 phages.
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<font size="4"> '''7/22/13''' </font>
<font size="4"> '''7/22/13''' </font>
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Prepared streaks of E. coli B. and BL21 using streaks from last Friday.
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Went over Children's book with the class, and talked about our next steps.
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We also dialyzed T4 that were extracted.
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<font size="4"> '''7/24/13''' </font>
<font size="4"> '''7/24/13''' </font>
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Ran a titer on our dialyzed T4 Phage. (experiment listed on 7.26)
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Worked on the wiki.  Prepared for our outreach to the community activity.
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<font size="4"> '''7/26/13''' </font>
<font size="4"> '''7/26/13''' </font>
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Took pictures of our purified phage using an EM. We noticed large amounts of crystalline structure in the solution that we believe to be CsCl. We believe that increasing the dialysis time will help purify the CsCl better and remove these crystalline structures.  
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We performed a titer to make sure our phage was a high enough concentration to take an EM of. We looked at the results from the titer today.
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.26 Phage Titer |7.26 Phage Titer]]
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<font size="4"> '''7/29/13''' </font>
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Worked on the wiki. We are waiting for our appointment with the TEM before continuing.
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<font size="4"> '''7/31/13''' </font>
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: Took TEM pictures of our phage
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:[[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.31 EM Pictures |7.31 EM Pictures]]
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Latest revision as of 00:04, 28 September 2013


Phage Purification July Notebook: July 16 - July 31 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



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7/17/13

Ran CsCl gradient for T4 group.

7.17 T4 CsCl Gradient


7/19/13

Discussed our results from 7.17 with the large phage group. we will be taking TEM pictures of the potentially mutated T4 phages.

7/22/13

Went over Children's book with the class, and talked about our next steps. We also dialyzed T4 that were extracted.


7/24/13 Ran a titer on our dialyzed T4 Phage. (experiment listed on 7.26)


7/26/13

We performed a titer to make sure our phage was a high enough concentration to take an EM of. We looked at the results from the titer today.

7.26 Phage Titer


7/29/13 Worked on the wiki. We are waiting for our appointment with the TEM before continuing.



7/31/13

Took TEM pictures of our phage
7.31 EM Pictures


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