Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/8.12CsClGradient
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+ | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
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'''III) Reagants Used''' | '''III) Reagants Used''' | ||
- | : | + | : T7 mutant phage |
: CsCl | : CsCl | ||
: dialysis tubing | : dialysis tubing | ||
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: Create different concentrations of CsCl solutions to create a gradient. | : Create different concentrations of CsCl solutions to create a gradient. | ||
- | :: | + | :: 1.1 - 2 mL |
- | + | ::1.2 - 2 mL | |
- | :: | + | ::1.25 - 2 mL |
- | :: | + | ::1.3 - 2 mL |
- | + | ::1.35 - 2mL | |
- | :: | + | ::1.4 - 2 mL |
- | :: | + | ::1.5 - 3 mL |
- | :: | + | ::1.6 - 2 mL |
- | :: | + | |
- | :: | + | |
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: Layer the gradient into two centrifuge tubes, using half of the total volume created for each tube. For example, add 2 mL of 1.3 density to one tube and the other 2 mL to another tube. | : Layer the gradient into two centrifuge tubes, using half of the total volume created for each tube. For example, add 2 mL of 1.3 density to one tube and the other 2 mL to another tube. | ||
: Fill the remaining space in the tube with phage suspension buffer to the top. | : Fill the remaining space in the tube with phage suspension buffer to the top. | ||
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'''V) Results''' | '''V) Results''' | ||
: The Phage did not band anywhere | : The Phage did not band anywhere | ||
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+ | [[File:Phage12_2.jpg | thumb|none|alt=]] | ||
+ | [[File:Phage12_3.jpg | thumb|none|alt=]] | ||
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Latest revision as of 00:36, 28 September 2013
Phage Purification July - August Notebook: Experiments
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8.12 CsCl Gradient
I) Purpose
II) Expected Outcome
III) Reagants Used
V) Results
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