Team:NRP-UEA-Norwich/LabJournal/Floor2/Protocols
From 2013.igem.org
(Difference between revisions)
(11 intermediate revisions not shown) | |||
Line 18: | Line 18: | ||
<ul> | <ul> | ||
<li class='inactive'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich'><span>Home</span></a></li> | <li class='inactive'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich'><span>Home</span></a></li> | ||
- | <li class=' | + | <li class='inactive'><a href='#'><span>Team</span></a> |
<ul> | <ul> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Team'><span>Overview</span></a></li> | ||
<li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Team/Students'><span>Students</span></a></li> | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Team/Students'><span>Students</span></a></li> | ||
<li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Team/Advisors'><span>Advisors</span></a></li> | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Team/Advisors'><span>Advisors</span></a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class='has-sub'><a href=' | + | <li class='has-sub'><a href='#'><span>Project</span></a> |
<ul> | <ul> | ||
- | + | ||
- | <li | + | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Project'><span>Overview</span></a></li> |
- | + | ||
- | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class=' | + | <li class='inactive'><a href='#'><span>Results</span></a> |
<ul> | <ul> | ||
- | + | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Results'><span>Overview</span></a></li> | |
- | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Results/ | + | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Results/Floor1'><span>Floor 1</span></a></li> |
+ | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Results/Floor2'><span>Floor 2</span></a> | ||
+ | </li> | ||
+ | |||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class=' | + | <li class='inactive'><a href='#'><span>Human Practice</span></a> |
<ul> | <ul> | ||
- | + | ||
- | + | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice'><span>Overview</span></a></li> | |
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice/SoilSamples'><span>Soil Samples</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice/Forum'><span>Forum Event</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice/UEA50'><span>UEA 50</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice/OtherActivities'><span>Collaborations</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice/Outreach'><span>Outreach</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Media'><span>Relevant Media</span></a></li> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/HumanPractice/Registry'><span>Resource Library</span></a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li class='active'><a href= | + | <li class='active'><a href=#'><span>Lab Journal</span></a> |
<ul> | <ul> | ||
+ | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal'><span>Overview</span></a></li> | ||
<li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor1'><span>Floor 1</span></a></li> | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor1'><span>Floor 1</span></a></li> | ||
<li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2'><span>Floor 2</span></a></li> | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2'><span>Floor 2</span></a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
- | <li><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/ | + | |
- | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/ | + | |
+ | |||
+ | <li class='has-sub'><a href='#'><span>Attributions</span></a> | ||
+ | <ul> | ||
+ | |||
+ | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Attributions'><span>Overview</span></a></li> | ||
+ | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/Team/Acknowledgements'><span>Acknowledgements</span></a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class='last'><a href='https://2013.igem.org/Team:NRP-UEA-Norwich/LabSafety'><span> Lab Safety</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
+ | |||
Line 65: | Line 86: | ||
width: 290px; | width: 290px; | ||
height: 54px;"><ul class="menu"> | height: 54px;"><ul class="menu"> | ||
- | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/Protocols" class=" | + | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/Protocols" class="active"><span><font size="2">PROTOCOLS</span> </a></li> |
- | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/ | + | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/June" class="inactive"><span><font size="2">JUNE 2013</span></a></li> |
- | + | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/July"><span><font size="2">JULY 2013</span></a></li> | |
- | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/ | + | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/August"><span><font size="2">AUGUST 2013</span></a></li> |
- | + | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/September"><span><font size="2">SEPTEMBER 2013</span></a></li> | |
- | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/ | + | |
- | + | ||
- | <li><a href="https://2013.igem.org/Team:NRP-UEA-Norwich/LabJournal/Floor2/ | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 107: | Line 116: | ||
left:175px; | left:175px; | ||
top:341px; | top:341px; | ||
- | width: | + | width: 387px; |
- | height: | + | height: 476px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 116: | Line 125: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/a/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/a/ae/Agarose_Gel_Electrophoresis_-small.png" width="387" height="476";" /></div> |
<!-- AGAROSE END --> | <!-- AGAROSE END --> | ||
Line 122: | Line 131: | ||
<!--INOCULATIONS START--> | <!--INOCULATIONS START--> | ||
<div style="position:absolute; | <div style="position:absolute; | ||
- | left: | + | left:588px; |
- | top: | + | top:341px; |
- | width: | + | width: 387px; |
- | height: | + | height: 207px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 133: | Line 142: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/a/aa/Inoculations_-_small.png" width="387" height="207";" /></div> |
<!-- INOCULATIONS END --> | <!-- INOCULATIONS END --> | ||
Line 139: | Line 148: | ||
<!--ISOLATIONS START--> | <!--ISOLATIONS START--> | ||
<div style="position:absolute; | <div style="position:absolute; | ||
- | left: | + | left:588px; |
- | top: | + | top:573px; |
- | width: | + | width: 387px; |
- | height: | + | height: 641px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 150: | Line 159: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/3/3a/Isolation_of_DNA_-small.png" width="387" height="641";" /></div> |
<!-- ISOLATIONS END --> | <!-- ISOLATIONS END --> | ||
- | <!--PROTEIN EXPRESSION START--> | + | <!-- PROTEIN EXPRESSION START --> |
+ | |||
<div style="position:absolute; | <div style="position:absolute; | ||
left:175px; | left:175px; | ||
- | top: | + | top:842px; |
- | width: | + | width: 387px; |
- | height: | + | height: 491px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 167: | Line 177: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/b/b8/Protein_Expression_-_small.png" width="387" height="491";" /></div> |
- | <!-- PROTEIN EXPRESSION END --> | + | <!--PROTEIN EXPRESSION END --> |
- | <!-- | + | <!--RESTRICTION ENZYME START--> |
<div style="position:absolute; | <div style="position:absolute; | ||
- | left: | + | left:588px; |
- | top: | + | top:1239px; |
- | width: | + | width: 387px; |
- | height: | + | height: 295px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 184: | Line 194: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/4/43/Restriction_Enzyme_Digest_small.png" width="387" height="295";" /></div> |
- | <!-- | + | <!-- RESTRICTION ENZYME END --> |
+ | |||
+ | <!-- SDS START --> | ||
- | |||
<div style="position:absolute; | <div style="position:absolute; | ||
left:175px; | left:175px; | ||
- | top: | + | top:1358px; |
- | width: | + | width: 387px; |
- | height: | + | height: 567px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 201: | Line 212: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/d/d8/10%25_SDS-POLYACRYLAMIDE_-_small.png" width="387" height="567";" /></div> |
- | <!-- | + | <!--SDS END --> |
+ | |||
+ | <!-- SD MUTAGENESIS START --> | ||
- | |||
<div style="position:absolute; | <div style="position:absolute; | ||
left:175px; | left:175px; | ||
- | top: | + | top:1950px; |
- | width: | + | width: 387px; |
- | height: | + | height: 568px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 218: | Line 230: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/a/ad/Site-Directed_Mutagenesis_small.png" width="387" height="568";" /></div> |
- | <!-- | + | <!--SD MUTAGENESIS END --> |
+ | |||
+ | <!--TRANSFORMATION START--> | ||
+ | <div style="position:absolute; | ||
+ | left:588px; | ||
+ | top:1559px; | ||
+ | width: 387px; | ||
+ | height: 371px; | ||
+ | margin: 0 auto; | ||
+ | display:block; | ||
+ | clear:both; | ||
+ | margin-top: 0px; | ||
+ | background: #d9eef8; | ||
+ | -webkit-border-radius: 5px; | ||
+ | -moz-border-radius: 5px; | ||
+ | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/5/53/Transformation_small.png" width="387" height="371";" /></div> | ||
+ | |||
+ | <!-- TRANSFORMATION END --> | ||
<!--WESTERN BLOT START--> | <!--WESTERN BLOT START--> | ||
<div style="position:absolute; | <div style="position:absolute; | ||
- | left: | + | left:588px; |
- | top: | + | top:1955px; |
- | width: | + | width: 387px; |
- | height: | + | height: 621px; |
margin: 0 auto; | margin: 0 auto; | ||
display:block; | display:block; | ||
Line 235: | Line 264: | ||
-webkit-border-radius: 5px; | -webkit-border-radius: 5px; | ||
-moz-border-radius: 5px; | -moz-border-radius: 5px; | ||
- | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/ | + | border-radius: 10px"><img src="https://static.igem.org/mediawiki/2013/d/df/Western_Blot_small.png" width="387" height="621";" /></div> |
<!-- WESTERN BLOT END --> | <!-- WESTERN BLOT END --> | ||
- | |||
<!-- PROTOCOLS END --> | <!-- PROTOCOLS END --> | ||
- | <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> | + | <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> |
</html> | </html> |
Latest revision as of 13:04, 3 October 2013