Team:BYU Provo/Safety

From 2013.igem.org

(Difference between revisions)
(→Risks)
 
(27 intermediate revisions not shown)
Line 13: Line 13:
|- valign="top"
|- valign="top"
-
| style="width: 20%; background-color: transparent;"|
+
| style="width: 22%; background-color: transparent;"|
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Attribution|Attributions]]
+
: [[Team:BYU_Provo/Safety#Approval|Approval]]
-
: [[Team:BYU_Provo/Collaboration|Collaborations]]
+
: [[Team:BYU_Provo/Safety#Overview|Overview]]
-
: [[Team:BYU_Provo/Safety|Safety]]
+
: [[Team:BYU_Provo/Safety#Safety_Form|Safety Form]]
-
 
+
-
: [[Team:BYU_Provo/Gallery|Galleries]]
+
</font>
</font>
-
| style="width: 81%; background-color: transparent;"|
+
| style="width: 78%; background-color: transparent;"|
<font face="Calibri" size="3">
<font face="Calibri" size="3">
-
__NOTOC__==Organism Used ==
+
__NOTOC__==Approval==
-
 
+
-
<to be deleted>Use this page to answer the questions on the  [[Safety | safety page]].
+
-
 
+
-
===Chassis Organisms===
+
-
 
+
-
{| class="wikitable"
+
-
| No || Species || Strain No/Name || Risk Group || Source Link || Disease Risk
+
-
|-
+
-
| 1 || Bacteriophage T4Do  || T4 || 1 ||  ||
+
-
|-
+
-
| 2 || Bacteriophage T7  || T7 || 1 || ?? other phage||
+
-
|-
+
-
| 3 || ''E. coli''  || B || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
+
-
|-
+
-
| 4 || ''E. coli''  || BL21 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
+
-
|-
+
-
| 5 || ''E. coli''  || TT9901 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
+
-
|-
+
-
| 6 || ''E. coli''  || TT9907 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
+
-
|-
+
-
| 7 || ''E. coli''  || TT25281 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
+
-
|-
+
-
| 8 || ''E. coli'' K12  || W3110 || 1 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia&species=coli ''E coli''] ||
+
-
|-
+
-
| 9 || ''Vibrio Cholerae''  || ATCC14035 || 2 || [http://www.absa.org/riskgroups/bacteriasearch.php?genus=Vibrio&species=Cholerae ''Vibrio Cholerae''] ||
+
-
|}
+
-
 
+
-
===Highest Risk Group Listed===
+
-
 
+
-
Greater than 1. For more information, please refer to
+
-
 
+
-
*  [[Team:BYU Provo/Safety#Biosafety_Form_Part_2|iGEM 2013 Biosafety Form Part 2]]
+
-
 
+
-
<html>
+
-
<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
+
-
</html>
+
-
 
+
-
<br>
+
-
 
+
-
 
+
-
==Coding Region Used==
+
-
 
+
-
<html>
+
-
<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
+
-
</html>
+
-
 
+
-
<br>
+
-
 
+
-
==Risks==
+
-
 
+
-
===Do the biological materials used in your lab work pose risks to the safety and health of team members or others working in the lab===
+
-
Yes. ''Vibrio cholerae'' can pose a risk to team member safety if ingested, handled improperly, or improperly disposed of (causing accidental exposure).
+
-
 
+
-
=== Do the biological materials used in your lab work pose risks to the safety and health of the general public, if released by design or by accident? ===
+
-
There are no plans for release of anything harmful. The safety practices, including proper disposal, were designed after consultation with a ''V. cholerae'' and bacterial pathogen expert, [http://lifesciences.byu.edu/~rar5 Dr. Richard Robison], BYU.
+
-
 
+
-
===Do the biological materials used in your lab work pose risks to the environment, if released by design or by accident?===
+
-
There are no risks associated with the environment.
+
-
 
+
-
===Do the biological materials used in your lab work pose risks to security through malicious misuse by individuals, groups, or countries?===
+
-
We believe our safety practices in the lab prevent malicious misuse by individuals, groups, and countries.
+
-
 
+
-
===If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?===
+
-
There would be no known risks. Our chassis organism is a BSL 1 E. coli. Of course caution must be used in any case. The bacteriophages we used also must be tested before any human use. 
+
-
 
+
-
<html>
+
-
<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
+
-
</html>
+
-
 
+
-
<br>
+
-
 
+
-
==Safety Precautions==
+
-
<html>
+
Safety forms were approved on 9/20/13 by David Lloyd and Julie McNamara.
-
<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
+
-
</html>
+
-
<br>
+
==Overview ==
-
==Biosafety Form Part 2==
+
Brigham Young University has set protocols aimed at ensuring student safety in the laboratory. Students are required to wear long pants and close-toed, waterproof shoes and follow safety precautions for working with chemicals and biological agents (protective clothing including gloves, etc.). They are prohibited from eating in laboratory rooms. The labs are kept locked when not in use and are subjected to random inspections to ensure that safety procedures are followed.
-
<html>
+
All biological agents we are working with are BSL1 (E. coli and various phages) or BSL2 (''Vibrio cholera'', ATCC 14035). Dr. Julianne Grose, our instructor, has received BYU approval to work with BSL-2 reagents. All students involved have been trained to handle cholera safely. We have followed current safety measures for disposing of biological samples in biohazard waste as well as cleaning glassware and desktops with bleach and/or ethanol.
-
<a href="https://2013.igem.org/Team:BYU_Provo/Safety" style="display:block;float:right;">Return to top >> </a>
+
-
</html>
+
-
<br>
+
All BioBrick parts created and used are non-hazardous and have been contained to the lab.
 +
The Institutional Biosafety Committee (IBC) at Brigham Young University supervises the use of recombinant DNA and handles other biological safety and hazard issues in experiments. Such use might involve constructing and handling DNA molecules in organisms. The IBC must review and approve all proposed experiments in advance of actual work. The link to there website is found [http://orca.byu.edu/ibc/ here]. We have submitted the necessary forms to our IBC and have received approval for our work.
-
==Form Download==
+
==Safety Form==
-
[[File:BYUSafetyForm1.pdf]]
+
[https://static.igem.org/mediawiki/2013/6/69/BYUSafetyForm1.pdf BYU Safety Form 1.pdf]
<html>
<html>

Latest revision as of 00:29, 27 September 2013


Safety


Approval
Overview
Safety Form

Approval

Safety forms were approved on 9/20/13 by David Lloyd and Julie McNamara.

Overview

Brigham Young University has set protocols aimed at ensuring student safety in the laboratory. Students are required to wear long pants and close-toed, waterproof shoes and follow safety precautions for working with chemicals and biological agents (protective clothing including gloves, etc.). They are prohibited from eating in laboratory rooms. The labs are kept locked when not in use and are subjected to random inspections to ensure that safety procedures are followed.

All biological agents we are working with are BSL1 (E. coli and various phages) or BSL2 (Vibrio cholera, ATCC 14035). Dr. Julianne Grose, our instructor, has received BYU approval to work with BSL-2 reagents. All students involved have been trained to handle cholera safely. We have followed current safety measures for disposing of biological samples in biohazard waste as well as cleaning glassware and desktops with bleach and/or ethanol.

All BioBrick parts created and used are non-hazardous and have been contained to the lab.

The Institutional Biosafety Committee (IBC) at Brigham Young University supervises the use of recombinant DNA and handles other biological safety and hazard issues in experiments. Such use might involve constructing and handling DNA molecules in organisms. The IBC must review and approve all proposed experiments in advance of actual work. The link to there website is found [http://orca.byu.edu/ibc/ here]. We have submitted the necessary forms to our IBC and have received approval for our work.

Safety Form

BYU Safety Form 1.pdf

Return to top >>




Retrieved from "http://2013.igem.org/Team:BYU_Provo/Safety"