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| | LP | | LP |
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| - | * Started approximately 20 mL of E coli B liquid culture overnight.LP | + | * Started approximately 20 mL of E coli B liquid culture overnight. |
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| - | * Discussed results from recent spot test for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.26 Mutagen Concentration Test|8.26 Mutagen Concentration Test - Eighth Protocol]]. Phage too spread out to effectively select for mutant phage. Decided to stop until the Phage Purification Team can get the wildtype T7 phage to band.
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| - | * Discussed plans and designs for team wiki with Darren and Keltzie.
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| - | * Started T7 propagation to generate enough phage for the Phage Purification Team to experiment with. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.
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| - | <font size="4"> '''9/5/13''' </font> | + | <font size="4"> '''9/20/13''' </font> |
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| | LP | | LP |
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| - | * Started approximately 10mL of E coli B liquid culture overnight. | + | * Performed "Plating to Check for Plaque Size" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]]. |
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| - | <font size="4"> '''9/6/13''' </font> | + | <font size="4"> '''9/23/13''' </font> |
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| | JL, LP | | JL, LP |
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| - | * Purified phage propagation sample from 9.4 via centrifugation (5 minutes at 3000 rpm) and cholorform. Spot tests were then performed to estimate phage titer. | + | * Performed "Plating to Check for Plaque Size 2" and "Checking for Plaque Viability from Step 5" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]]. |
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| - | * Took pictures of plates from [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]] using alpha imager.
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| - | * Divided up assignments for updating notebook.
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| - | <font size="4"> '''9/7/13''' </font> | + | <font size="4"> '''9/24/13''' </font> |
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| - | JL
| + | LP |
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| - | * Started approximately 15 mL E coli B liquid culture overnight. | + | * Started approximately 30 mL E coli B liquid culture overnight. |
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| - | * Worked on data analysis / picture processing for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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| - | <font size="4"> '''9/8/13''' </font> | + | <font size="4"> '''9/25/13''' </font> |
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| - | JL | + | JL, LP |
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| - | * Continued data analysis / picture processing for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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| - | * Performed preliminary titer in preparation for repeating the modeling experiment with T1 and T2. For specifics, please see [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]]. | + | * Performed "Checking for Plaque Viability 2" for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]]. |
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| | * Started approximately 25mL of E coli B liquid culture overnight | | * Started approximately 25mL of E coli B liquid culture overnight |
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| - | <font size="4"> '''9/9/13''' </font> | + | <font size="4"> '''9/26/13''' </font> |
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| - | JL, LP
| + | LP |
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| - | * Discussed analysis of modeling result using ImageJ.
| + | * Started approximately 25mL of E. coli B overnight. |
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| - | * Repeated the modeling procedure in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]] for T1 and T2. Incubation started at 4:30pm.
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| - | * Started a fresh round for phage propagation for the Phage Purification Team. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock. | + | |
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| - | * Divided up assignments for updating the wiki.
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| - | <font size="4"> '''9/10/13''' </font> | + | <font size="4"> '''9/27/13''' </font> |
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| - | JL, LP
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| - | * Took the T1 and T2 modeling plates our the incubation at 4:30pm.
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| - | * Started approximately 25mL of E coli B liquid culture overnight.
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| - | * Measured plaque sizes using ImageJ.
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| - | <br>
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| - | <font size="4"> '''9/11/13''' </font>
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| - | JL, LP
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| - | * Isolated the phage (from 9.9) by centrifuging down the bacteria, transferring the supernatant to a new 15mL centrifuge tube, and adding 900ul of chloroform to the supernatant.
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| - | * Took pictures of T1 and T2 plates for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]].
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| - | <br>
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| - | <font size="4"> '''9/12/13''' </font>
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| - | JL
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| - | * Spot test showed plaques up to -7. This indicate that the phage suspension solution we gave the Phage Purification Team for CsCl was at least 10E9 pfu/mL.
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| - | * Started approximately 30mL of E coli B liquid culture overnight.
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| - | <br>
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| - | <font size="4"> '''9/13/13''' </font>
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| - | JL, LP
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| - | * The Phage Purification Team has good news for us. They were able to observe banding in their latest CsCl gradient for T7. We thus decided to perform a new round of mutagenesis this weekend and hopefully selection next week.
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| - | * In preparation for mutagenesis tomorrow, we performed titers for 8.24 T7 stock.
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| - | * Started approximately 5mL of E coli B liquid culture overnight.
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| - | * Streaked out E coli B.
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| - | <font size="4"> '''9/14/13''' </font>
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| - | JL
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| - | * Autoclaved ddH<sub>2</sub>O, LB, x2 top agar.
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| - | * Used the autoclaved ddH<sub>2</sub>O to make adenine and uracil solution. Specifically,
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| - | : - 254mg of uracil was dissolved in 10mL of ddH<sub>2</sub>O to produce a uracil solution with a concentration of approximately 2.5mg/mL
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| - | : - 753mg of adenine hemisulfate dihydrate was dissolved in 10mL of ddH<sub>2</sub>O to produce a adenine solution with a concentration of approximately 5mg/mL
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| - | * Started approximately 5mL of E coli B liquid culture overnight.
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| - | <font size="4"> '''9/15/13''' </font>
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| | JL, LP | | JL, LP |
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| - | * Performed the mutagenesis procedure in [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]] | + | * Determined results for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]]. |
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| - | * Started approximately 20mL of E coli B liquid culture overnight | + | * Finished updating the wiki. |
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| - | * Started phage propagation | + | * Started approximately 30mL of E. coli B overnight. |
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| | <br> | | <br> |
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