Template:Kyoto/Notebook/Aug 26

From 2013.igem.org

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|1||100bp ladder||-
|1||100bp ladder||-
|-
|-
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|2||8/24 tetR aptamer 12_P||EcoRI&SpeI
+
|2||8/24 tetR aptamer 12_P||EcoRI & SpeI
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|-
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|4||8/24 tetR aptamer 12_1R||EcoRI&SpeI
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|4||8/24 tetR aptamer 12_1R||EcoRI & SpeI
|-
|-
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|6||8/24 tetR aptamer 12_M||EcoRI&SpeI
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|6||8/24 tetR aptamer 12_M||EcoRI & SpeI
|}
|}
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[[File:igku_xxbeforexx.xxx]]<br>
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[[File:Igku Aug26 Gel Extraction(N①)before.jpg]]<br>
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[[File:igku_xxafterxx.xxx]]<br>
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[[File:Igku Aug26 Gel Extraction(N②)after.jpg]]<br>
{| class="wikitable"
{| class="wikitable"
!Name||concentration[&micro;g/mL]||260/280||260/230
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|tetR aptamer 12_P(EcoRI&SpeI)||2.3||0.38||0.13
+
|tetR aptamer 12_P (EcoRI & SpeI)||2.3||0.38||0.13
|-
|-
-
|tetR aptamer 12_1R(EcoRI&SpeI)||12.0||0.63||1.19
+
|tetR aptamer 12_1R (EcoRI & SpeI)||12.0||0.63||1.19
|-
|-
-
|tetR aptamer 12_M(EcoRI&SpeI)||2.0||6.43||0.03
+
|tetR aptamer 12_M (EcoRI & SpeI)||2.0||6.43||0.03
|}
|}
</div>
</div>
 +
===Ligation===  
===Ligation===  
-
<!-- こっから -->
 
<div class="experiment">
<div class="experiment">
<span class="author">Ashida</span>
<span class="author">Ashida</span>
{| class="wikitable"
{| class="wikitable"
-
!Name||-?-
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!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
|-
|-
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|8/21 DT||5.0
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|experiment||8/21 DT||5.0||8/26 tetR aptamer 12_1R||2.0||3.5
|-
|-
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|8/26 tetR aptamer 12_1R||2.0
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|experiment||8/21 DT||5.0||8/22 pT181 atteniator||5.0||5.0
|-
|-
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|Ligation High buffer||3.5
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|experiment||8/21 DT||5.0||8/22 pT181 antisense||3.0||4.0
-
|}
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-
{| class="wikitable"
+
-
!Name||-?-
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|-
|-
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|8/21 DT||5.0
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|experiment||8/21 DT||5.0||8/22 Spinach||3.0||4.0
|-
|-
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|8/22 pT181 atteniator||5.0
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|experiment||8/22 Plac||4.0||8/22 pT181 attenuator||5.0||4.5
-
|-
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|Ligation High buffer||5.0
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-
|}
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{| class="wikitable"
+
-
!Name||-?-
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|-
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|8/21 DT||5.0
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-
|-
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|8/22 pT181 antisense||3.0
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-
|-
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|Ligation High buffer||4.0
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-
|}
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-
{| class="wikitable"
+
-
!Name||-?-
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-
|-
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|8/21 DT||5.0
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-
|-
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|8/22 Spinach||3.0
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-
|-
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|Ligation High buffer||4.0
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-
|}
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{| class="wikitable"
+
-
!Name||-?-
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-
|-
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|8/22 Plac||4.0
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-
|-
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|8/22 pT181 attenuator||5.0
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-
|-
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|Ligation High buffer||4.5
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-
|}
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{| class="wikitable"
+
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!Name||-?-
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-
|-
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|8/22 Pcon||5.0
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|-
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|8/22 pT181 attenuator||5.0
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-
|-
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|Ligation High buffer||5.0
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-
|}
+
-
{| class="wikitable"
+
-
!Name||-?-
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-
|-
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|8/22 Plac||4.0
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|-
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|8/22 pT181 antisense||8.0
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-
|-
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|Ligation High buffer||6.0
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-
|}
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-
{| class="wikitable"
+
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!Name||-?-
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|-
|-
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|8/22 Pcon||4.0
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|experiment||8/22 J23100||4.0||8/22 pT181 attenuator||5.0||4.5
|-
|-
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|8/22 pT181 antisense||8.0
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|experiment||8/22 Plac||4.0||8/22 pT181 antisense||8.0||6.0
|-
|-
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|Ligation High buffer||6.0
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|experiment||8/22 J23100||4.0||8/22 pT181 antisense||8.0||6.0
|}
|}
</div>
</div>
-
<!-- ここまでをコピーしてね -->
 
===Transformation===
===Transformation===

Latest revision as of 09:02, 24 September 2013

Contents

Aug 26

Gel Extraction

Matsumoto and Ashida

LaneDNAEnzyme
1100bp ladder-
28/24 tetR aptamer 12_PEcoRI & SpeI
48/24 tetR aptamer 12_1REcoRI & SpeI
68/24 tetR aptamer 12_MEcoRI & SpeI

Igku Aug26 Gel Extraction(N①)before.jpg
Igku Aug26 Gel Extraction(N②)after.jpg

Nameconcentration[µg/mL]260/280260/230
tetR aptamer 12_P (EcoRI & SpeI)2.30.380.13
tetR aptamer 12_1R (EcoRI & SpeI)12.00.631.19
tetR aptamer 12_M (EcoRI & SpeI)2.06.430.03

Ligation

Ashida

stateVectorInserterLigation High ver.2
experiment8/21 DT5.08/26 tetR aptamer 12_1R2.03.5
experiment8/21 DT5.08/22 pT181 atteniator5.05.0
experiment8/21 DT5.08/22 pT181 antisense3.04.0
experiment8/21 DT5.08/22 Spinach3.04.0
experiment8/22 Plac4.08/22 pT181 attenuator5.04.5
experiment8/22 J231004.08/22 pT181 attenuator5.04.5
experiment8/22 Plac4.08/22 pT181 antisense8.06.0
experiment8/22 J231004.08/22 pT181 antisense8.06.0

Transformation

No name

NameSampleCompetent CellsTotalPlate
8/26 tetR aptamer 12_1R-DT1µL10µL11µLCP
8/26 pT181 attenuator-DT1µL10µL11µLCP
8/26 pT181 antisense-DT1µL10µL11µLCP
8/26 Spinach-DT1µL10µL11µLCP
8/26 Plac-pT181 attenuator1µL10µL11µLCP
8/26 Pcon-pT181 attenuator1µL10µL11µLAmp
8/26 Plac-pT181 antisense1µL10µL11µLCP
8/26 Pcon-pT181 antisense1µL10µL11µLAmp
Pλ-RBS-luxI-DT1µL10µL11µAmp
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