Team:EPF Lausanne

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'''Taxi.Coli: smart drug delivery'''
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<br><br><br>
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We are proud to participate in iGEM 2013 and are excited to present<br>
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'''Taxi.Coli'''.
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<br>
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Taxi.Coli is a highly flexible and adaptable smart drug delivery system.<br>
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We want to use E.coli as a ''Taxi'', that expresses streptavidin on its surface as ''Seats'' for the ''Passengers'', which are the biotinylated nanoparticles that contain a drug. Once Taxi.Coli arrives at ''the Address'', a specific environment, it will produce an enzyme that degrades the nanoparticles, releasing the incorporated drug ''the passenger gets out of the car''. <br>Our effort will be limited to a proof of principle, but one can imagine a wide range of future applications.
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Researchers all over the world strive to improve the effectiveness of drugs. Emergent strategies such as "Targeted drug delivery" allow a more precise and localized administration of drugs. The aim of this method is to increase drug concentration in a specific location (e.g. a tumor); yet decreasing secondary effects on healthy tissues. This method reduces the frequency and dose of drug administration.
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EPFL team’s goal this year is to suggest a drug delivery strategy. The key word of this project is “adaptability”. Our motivation is to explore a way of using E.Coli as a drug carrier in a way that is highly modular; thereby suiting several applications and possibilities of disease treatment.
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==Achievements:==
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- We can produce biotinylated nanoparticles<br>
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- We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles<br>
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- We can engineer E.coli to sense different values of pH<br>
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- We can make E.coli express superfolded GFP constitutively<br>
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- We can excite other students about synthetic biology<br>
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Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]!
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<h2>Medal requirements</h2>
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<h3>Bronze medal</h3>
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  <li>We registered the team and had fun in the competition.</li>
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  <li>We filled in the Judging form.</li>
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  <li>We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.</li>
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  <li>We plan to present a poster and talk at the iGEM Jamboree in Lyon.</li>
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  <li>We designed and submitted the following Biobricks BBa_K1111001, BBa_K1111002, BBa_K111103, BBa_K1111004, BBa_K1111005, BBa_K1111006, BBa_K1111007, BBa_K1111008, BBa_K1111009, BBa_K1111010, BBa_K1111011, BBa_K1111012, BBa_K1111013, BBa_K1111014.</li>
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<h3>Silver medal</h3>
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  <li>We experimentally validated that the Biobrick BBa_K1111005 works as expected.</li>
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  <li>We documented all our parts on the iGEM Registry of Standard Biological Parts.</li>
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  <li>We investigated the safety issues which could be raised by our project. Take a look at our safety page.</li>
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<h3>Gold medal</h3>
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  <li>We improved the function of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. This will help future teams to use it.</li>
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  <li>We prepared an amazing synthetic biology kit which will be used at public events and high school visits at EPFL. Take a look at our Outreach page. </li>
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<li>We organized a women and engineering's day at our university to promote women in science. </li>
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<h3>European Jamboree Results</h3>
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  <li>SILVER MEDAL</li>
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  <li>ADVANCE  TO WORLD CHAMPIONSHIP</li>
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Using the principles of synthetic biology we will bond nanoparticles containing a drug to E.coli using the biotin-streptavidin interaction. The engineered E.coli will target the site where the drug is required. Upon stimuli it starts to release a protease degrading the nanoparticles and hence delivers the drug.
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== Thanks to our sponsors ==
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Many thanks to our sponsors who made it possible for us to take part in this amazing competition.
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[[File:Team-EPF-Lausanne sponso.jpg|center|550px]]
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Latest revision as of 00:06, 29 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header




We are proud to participate in iGEM 2013 and are excited to present
Taxi.Coli.
Taxi.Coli is a highly flexible and adaptable smart drug delivery system.
We want to use E.coli as a Taxi, that expresses streptavidin on its surface as Seats for the Passengers, which are the biotinylated nanoparticles that contain a drug. Once Taxi.Coli arrives at the Address, a specific environment, it will produce an enzyme that degrades the nanoparticles, releasing the incorporated drug the passenger gets out of the car.
Our effort will be limited to a proof of principle, but one can imagine a wide range of future applications.





Project
Team
Safety
Outreach





Achievements:

- We can produce biotinylated nanoparticles
- We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles
- We can engineer E.coli to sense different values of pH
- We can make E.coli express superfolded GFP constitutively
- We can excite other students about synthetic biology



Please also visit our Facebook page!


Medal requirements

Bronze medal

  • We registered the team and had fun in the competition.
  • We filled in the Judging form.
  • We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.
  • We plan to present a poster and talk at the iGEM Jamboree in Lyon.
  • We designed and submitted the following Biobricks BBa_K1111001, BBa_K1111002, BBa_K111103, BBa_K1111004, BBa_K1111005, BBa_K1111006, BBa_K1111007, BBa_K1111008, BBa_K1111009, BBa_K1111010, BBa_K1111011, BBa_K1111012, BBa_K1111013, BBa_K1111014.

Silver medal

  • We experimentally validated that the Biobrick BBa_K1111005 works as expected.
  • We documented all our parts on the iGEM Registry of Standard Biological Parts.
  • We investigated the safety issues which could be raised by our project. Take a look at our safety page.

Gold medal

  • We improved the function of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. This will help future teams to use it.
  • We prepared an amazing synthetic biology kit which will be used at public events and high school visits at EPFL. Take a look at our Outreach page.
  • We organized a women and engineering's day at our university to promote women in science.


    • European Jamboree Results

    • SILVER MEDAL
    • ADVANCE TO WORLD CHAMPIONSHIP



    Thanks to our sponsors

    Many thanks to our sponsors who made it possible for us to take part in this amazing competition.

    Team-EPF-Lausanne sponso.jpg



Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne"