Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/6.10PhagePreparation

(Difference between revisions)

Latest revision as of 00:29, 28 September 2013

Phage Purification May - June Notebook: Experiments

Phage Purification

March-April

6.10 Phage Preparation

I) Purpose

Grow phage to practice purification procedures.

II) Expected Outcome

Viable stocks of both T4 and T7.

III) Reagants Used

W3110 and BL21 freezer stocks

LB

T4 and T7 phage stocks

IV) Actual Procedure

Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37^◦ C overnight.

Infect W3110 with 100 µL T4 and BL21 with 100 µL T7.

Let both cultures sit overnight at 37^◦ C.

V) Results

We created phage cultures to practice the purification procedure.

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 : W3110 and BL21 freezer stocks
 : LB
-: T4 and T7 stocks
+: T4 and T7 phage stocks
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 : Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37<sup>◦</sup> C overnight.
-: Infect W3110 with 100 microL T4 and BL21 with 100 microL T7.
+: Infect W3110 with 100 µL T4 and BL21 with 100 µL T7.
 : Let both cultures sit overnight at 37<sup>◦</sup> C.