Team:Manchester/Project

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== Objectives ==
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<title> Safety </title>
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# to introduce a new, more eco-friendly alternative to palm oil, using ''Escherichia coli''.
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<br>
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== Overview ==
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=== <u>Palm oil</u> ===
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Palm oil is a huge business. From food products such as margarine and chocolate, to cosmetics and even applications in biodiesel, palm oil is the most widely used vegetable oil in the world, and its demand is only increasing. But what most of the manufacturers of these products fail to address is the massive devastation caused by ripping up rainforests in order to make room for oil palm plantations. Not only does this mass deforestation damage the planet by increasing carbon emissions and decreasing the amount of CO2 taken up from the atmosphere, it also destroys the homes and habitats of thousands of endangered animals, including orangutans and tigers.
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That's where we come in.
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=== <u>Our project</u> ===
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Palm oil is made up of four major components: palmitic acid, stearic acid, oleic acid, and linoleic acid. These four fatty acids are fractionated from crude palm oil and used in various different applications - stearic acid is used primarily in cosmetics, where oleic acid is an important oil used in cooking, for example.
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The biosynthesis of fatty acids has been studied extensively in ''E. coli'', which makes this the ideal chassis for our project. Our aim is to exploit the pathway of naturally occurring saturated fatty acids (palmitic (C16:0) and stearic acid (C18:0)), and then insert non-native genes to introduce double bonds in stearic acid, to yield oleic (C18:1, Δ9) and linoleic acid (C18:2, Δ9,12). Producing palm oil in this way would remove the need to plant oil palm trees, and so would reduce the amount of deforestation occurring.
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=== <u>References</u> ===
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# http://www.greenpeace.org.uk/forests/palm-oil
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# http://wwf.panda.org/what_we_do/footprint/agriculture/palm_oil/
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# Cottrell, RC (1991). "Introduction: nutritional aspects of palm oil". ''The American journal of clinical nutrition'', '''53''' (4 Suppl.), 989S–1009S.
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<div class="banner">
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  <div class="igem">  
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  <a href="https://2013.igem.org/Main_Page">
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          <img src="https://static.igem.org/mediawiki/2013/3/3f/Igem.png">
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          </a>
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    <a><img src="https://static.igem.org/mediawiki/2013/8/87/LOGO1.jpg"></a>
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    <a><img src="https://static.igem.org/mediawiki/2013/9/90/ProjectMan.png"></a>
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<div class="global">
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<div class="wrapper" >
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<div class="mtextbox">
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  <b><u>Key Achievements</u></b><br>
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• Created three biobricks<br>
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• Used an expression vector based on standard parts to successfully characterise biobricks<br>
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• Improvement of a biobrick
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</div>
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        <div id="content1">
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            <p>
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The mass deforestation in Indonesia and Malaysia to make room for oil palm plantations is one of the biggest issues of our time. Not only does it result in the extinction of many endangered species, it’s also having a massive impact on the amount of CO<sub>2</sub> released into the atmosphere. That’s where we come in...
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        </p>
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                        <img src="https://static.igem.org/mediawiki/2013/7/7e/Aimresized.jpg" height="230" width="150"/>     
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<a href="https://2013.igem.org/Team:Manchester/Overview" class="myButton" > PROJECT OVERVIEW </a>
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  </div>
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  <div id="content2">
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  <img src="https://static.igem.org/mediawiki/2013/f/fd/Safety3.jpg" height="230" width="150"/>     
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            <p>
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Promoting safety within the lab, and also within the wider synthetic biology community, is paramount to the success of this research area. Here we consider how lab work can be made as safe as possible, and also how we would ensure our project remains safe if it became a commercial success.
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</p>         
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            <a href="https://2013.igem.org/Team:Manchester/Safety" class="myButton" > SAFETY </a>
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  </div>
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    <div id="content3">
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            <p>
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Over the course of the summer we documented our progress weekly, both as a way for the team to remain as organised as possible and also as a way to allow future teams to see how we developed the project through the weeks. Also sometimes it is nice to show off.
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            </p>
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                        <img src="https://static.igem.org/mediawiki/2013/c/cb/Notebookresized.jpg" height="230" width="150"/>     
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<a href="https://2013.igem.org/Team:Manchester/Notebook" class="myButton" >NOTEBOOK </a>
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  </div>
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  <img src="https://static.igem.org/mediawiki/2013/3/30/Labbook3.jpg" height="230" width="150"/>
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            <p>
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Can’t have an iGEM team without a lab book! Here you will find a list of protocols, some general and some more project-specific, that we have been using during the project.  
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</p>         
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            <a href="https://2013.igem.org/Team:Manchester/LabBook" class="myButton" > LAB BOOK </a>
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  </div>
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        <div id="content5">
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            <p>
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We think the work we’ve done during this uncharacteristically hot summer has what it takes to win some medals/prizes! Click here to see our completed Judging Form and a medalling checklist.
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          </p>
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                        <img src="https://static.igem.org/mediawiki/2013/2/29/Judge.jpg" height="230" width="150"/> 
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<a href="https://2013.igem.org/Team:Manchester/Judging" class="myButton" > JUDGING</a>
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  </div>
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  <div id="content6">
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  <img src="https://static.igem.org/mediawiki/2013/9/9f/Projectpartspageresized.jpg" height="230" width="150"/>
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            <p>
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Click here to see the list of the BioBricks we have sent in to the Registry of Standard Biological Parts, in addition to detailed information about our characterisation.
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</p>         
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            <a href="https://2013.igem.org/Team:Manchester/Parts" class="myButton" > PARTS CONTRIBUTION </a>
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  </div>
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</div>
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</div>
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</body>
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</html>

Latest revision as of 01:34, 5 October 2013

page

Top

Safety

Key Achievements
• Created three biobricks
• Used an expression vector based on standard parts to successfully characterise biobricks
• Improvement of a biobrick

The mass deforestation in Indonesia and Malaysia to make room for oil palm plantations is one of the biggest issues of our time. Not only does it result in the extinction of many endangered species, it’s also having a massive impact on the amount of CO2 released into the atmosphere. That’s where we come in...

PROJECT OVERVIEW

Promoting safety within the lab, and also within the wider synthetic biology community, is paramount to the success of this research area. Here we consider how lab work can be made as safe as possible, and also how we would ensure our project remains safe if it became a commercial success.

SAFETY

Over the course of the summer we documented our progress weekly, both as a way for the team to remain as organised as possible and also as a way to allow future teams to see how we developed the project through the weeks. Also sometimes it is nice to show off.

NOTEBOOK

Can’t have an iGEM team without a lab book! Here you will find a list of protocols, some general and some more project-specific, that we have been using during the project.

LAB BOOK

We think the work we’ve done during this uncharacteristically hot summer has what it takes to win some medals/prizes! Click here to see our completed Judging Form and a medalling checklist.

JUDGING

Click here to see the list of the BioBricks we have sent in to the Registry of Standard Biological Parts, in addition to detailed information about our characterisation.

PARTS CONTRIBUTION

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