Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period9/Explist

From 2013.igem.org

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: [[Team:BYU_Provo/Phage_Purification|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="4"> '''7.3 EM Phage Preperation''' </font>
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<font size="4"> '''7.17 CsCl Gradient''' </font>
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: This is the procedure used to prepare samples of purified phage for electron microscopy imaging (EM).
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: Trying out a new gradient for T4 purification.
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.3 EM Phage Preparation |7.3 EM Phage Preparation]]
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.17 CsCl Gradient|7.17 CsCl Gradient]]
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<font size="4"> '''7.5 Bacterial Preparation''' </font>
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<font size="4"> '''7.26 T4 Titer''' </font>
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: Streaking cultures of bacteria for use in phage propagation
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: We ran a titer on our mutated phage from the CsCl gradient to make sure we had a high enough concentration of phage for an EM.  
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.5 Bacterial Preparation|7.5 Bacterial Preparation]]
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<font size="4"> '''7.12 Bacterial Preparation''' </font>
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:Streaking cultures of bacteria for use in phage propagation
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:Detailed Procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.12 Bacterial Preparation|7.12 Bacterial Preparation]]
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<font size="4"> '''7.17 CsCl Gradient''' </font>
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: Trying out a new gradient for T4 purification.
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.17 CsCl Gradient|7.17 CsCl Gradient]]
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Summerexp/Period1/Exp/7.26 Phage Titer |7.26 Phage Titer]]
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Latest revision as of 00:35, 28 September 2013


Phage Purification July - August Notebook: July 16 - July 31 Experiment Listing



Phage Purification
March-April
May-June
July-August
September-October

7.17 CsCl Gradient

Trying out a new gradient for T4 purification.
Detailed procedure: 7.17 CsCl Gradient


7.26 T4 Titer

We ran a titer on our mutated phage from the CsCl gradient to make sure we had a high enough concentration of phage for an EM.
Detailed procedure: 7.26 Phage Titer


7.31 EM Pictures

These are the picture results from the EM.
7.31 EM Pictures


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