Team:BYU Provo/Notebook/Phage Purification/Springexp/Period2/Explist

From 2013.igem.org

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: [[Team:BYU_Provo/Small_Phage|Overview]]
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: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
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<font size="4"> '''5.20 Titer''' </font>
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The titer will allow us to know if our purification resulted in viable phage.
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Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.20Titer|5.20 Titer]]
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<font size="4"> '''5.22 T4 Titer''' </font>
<font size="4"> '''5.22 T4 Titer''' </font>
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The first step in phage purification.  This will purify phage using PEG 8000.
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The T4 titer will allow us to know if our purification resulted in viable T4 phage.
Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.22T4Titer|5.22 T4 Titer]]
Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.22T4Titer|5.22 T4 Titer]]

Latest revision as of 00:11, 28 September 2013


Phage Purification May - June Notebook: May 13 - May 26 Experiment Listing



Phage Purification
March-April
May-June
July-August
September-October

5.20 Titer

The titer will allow us to know if our purification resulted in viable phage.

Detailed procedure: 5.20 Titer


5.22 T4 Titer

The T4 titer will allow us to know if our purification resulted in viable T4 phage.

Detailed procedure: 5.22 T4 Titer


5.24 CsCl Gradient Phage Purification

This procedure uses a CsCl gradient to purify phage at a high level of purification.

Detailed procedure: 5.24 CsCl Gradient Phage Purification


5.26 PEG Purification

The first step in phage purification. This will purify phage using PEG 8000.

Detailed procedure: 5.26 PEG Purification