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| - | <title>BostonU iGEM Team: Welcome</title> | + | <h2>Protocols</h2> |
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| - | </head>
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| - | <body class="basiclayout">
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| - | <div id="bu-wellesley_wiki_content">
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| - | <div id="igemlogo">
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| - | <a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/1/13/Igemwhite_back.png" alt="iGEM 2012 main page" style="position: absolute; top: 10px; left: 10px;"width="90"></a>
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| - | </div>
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| - | <p style="text-align:center;"><a href="https://2013.igem.org/Team:BostonU"><img src="https://static.igem.org/mediawiki/2013/2/2c/Marsh.png" width="800px" length="200px"></a></p>
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| - | <ul id="nav">
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| - | <li><a href="#">Our Team</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Team">Team</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Social">Summer Fun</a></li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="#">Project</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/MoCloChara">Introduction to MoClo and Characterization</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/QS">Quorum Sensing</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/HK">Histidine Kinase</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/ML">MoClo Library</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/DataSheet">Data Sheets App</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Results ">Results Summary</a></li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="#">Achievements</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Data">Data Collected</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>
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| - | </ul>
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| - | </li>
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| - |
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| - | <li><a href="#">Notebook</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Clotho">Clotho and Eugene</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/PR">Pigeon and Raven</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/NotebookQS">Quorum Sensing Notebook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/NotebookHK">Histidine Kinase Notebook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/NotebookML">MoClo Library Notebook</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/TroubleShooting">Trouble Shooting</a></li>
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| - | </ul>
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| - | </li>
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| - | <li><a href="#">Considerations</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Safety">Safety</a></li>
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| - | </ul>
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| - | <li><a href="#">Acknowledgements</a>
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| - | <ul>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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| - | <li><a href="https://2013.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
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| - |
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| - | </ul>
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| - | </li>
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| - | </ul>
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| - |
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| - | <br>
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| - | <h4>Protocols</h4>
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| | <h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p> | | <h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p> |
| | We hope they help anyone interested in our experiments to successfully repeat them. | | We hope they help anyone interested in our experiments to successfully repeat them. |
| - | <br> | + | <br><br> |
| - | <br> | + | |
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| - | <br> | + | <a href="https://static.igem.org/mediawiki/2013/7/7e/NanoDrop_DNA_Quantification.xls">DNA Quantification</a><br> |
| - | < li><a href="https://static.igem.org/mediawiki/ 2012/ 0/ 0b/ Phusion_PCRblank.xls"> Phusion PCR </a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/2/2c/Epoch_Gel_extraction.xls">Gel Extraction</a><br> |
| - | <li><a href="https://static.igem.org/mediawiki/ 2012/ c/ cb/ Transformation_Gold_Bioline_cells.xls"> Transformation with Gold Bioline Cells</a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/3/3b/Epoch_Miniprep.xls">Miniprep</a><br> |
| - | < li><a href="https://static.igem.org/mediawiki/ 2012/0/ 05/ Restriction_Digest.xls"> Restriction Digest </a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/6/6e/Epoch_PCR_cleanup.xls">PCR Cleanup</a><br> |
| - | <li><a href="https://static.igem.org/mediawiki/ 2012/ 8/ 8e/ Qiagen_QIAquick_Gel_extraction.xls"> Gel Extraction </a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/0/01/Ligations.xls">Ligation</a><br> |
| - | <li><a href="https://static.igem.org/mediawiki/ 2012/ 5/ 58/ Qiagen_QIAprep_Miniprep.xls"> Miniprep </a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/b/bd/Media_Preparation_LB_broth_agar_Gels.xls">Media Prep</a><br> |
| - | <li><a href="https://static.igem.org/mediawiki/ 2012/ 7/ 7e/ NanoDrop_DNA_Quantification.xls"> DNA Quantification </a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/6/61/MoClo_Level_0.xls">MoClo Level 0 Reaction</a><br> |
| - | < li><a href="https://static.igem.org/mediawiki/ 2012/ b/ bd/ Media_Preparation_LB_broth_agar_Gels.xls"> Media Prep </a>< /p> | + | <a href="https://static.igem.org/mediawiki/2013/4/4d/MoClo_Level_1_and_2.xls">MoClo Level 1 and 2 Reactions</a><br> |
| - | < li><a href="https://static.igem.org/mediawiki/ 2012/ 1/ 1f/ Ligation_Regular_and_MoClo.xls"> Ligation </a></ p> | + | <a href="https://static.igem.org/mediawiki/2013/d/dc/Phusion_PCR.xls">Phusion PCR</a><br> |
| | + | <a href="https://static.igem.org/mediawiki/2013/0/05/Restriction_Digest.xls">Restriction Digest</a><br> |
| | + | <a href="https://static.igem.org/mediawiki/2013/8/8e/Restriction_Digest_mapping_10uLs.xls">Restriction Digest 10 µL mapping</a><br> |
| | + | <a href="https://static.igem.org/mediawiki/2013/8/8b/Transformation.xls">Transformation</a><br> |
| | + | </h7> |
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