Template:Kyoto/Notebook/Aug 10

From 2013.igem.org

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{| class="wikitable"
{| class="wikitable"
!Lane||Sample
!Lane||Sample
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|4||NC
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!Sample||medium
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|8/9 BBa-J23106||LB(+amp)  
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|8/9 BBa-J23106||LB(+Amp)  
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|8/9 BBa-J23100-1||LB(+amp)  
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|8/9 BBa-J23100-1||LB(+Amp)  
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|8/9 BBa-J23100-2||LB(+amp)  
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|8/9 BBa-J23100-2||LB(+Amp)  
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|8/9 BBa-J44000-1||LB(+amp)  
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|8/9 BBa-J44000-1||LB(+Amp)  
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|-
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|8/9 BBa-J44000-2||LB(+amp)  
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|8/9 BBa-J44000-2||LB(+Amp)  
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|0.4M NaOHaq||500&micro;L
|0.4M NaOHaq||500&micro;L
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|-
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|Agarose Pouder||2g　
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|Agarose Pouder||2g
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|Ampicillin||40&micro;L
|Ampicillin||40&micro;L

Latest revision as of 03:40, 28 September 2013

Contents

Aug 10

Colony PCR

Nakamoto

Sample
8/9 BBa_J44000 -(1)
8/9 BBa_J44000 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec30 sec30 cycles
Sample
8/9 BBa_J23106
8/9 BBa_J23100 -(1)
8/9 BBa_J23100 -(2)
NC
PreDenatureDenatureAnnealingExtensioncycle
94 °C94 °C55 °C68 °C--
5 min30 sec30 sec1 min30 cycles

Electrophoresis

Tatsui and Nakamoto

LaneSample
100bp ladder
48/9 BBa_J44000 -1
58/9 BBa_J44000 -2
6NC

Igku Aug10 Electrophoresis BBa 1.jpg

LaneSample
1kbp ladder
18/9 BBa_J23106
28/9 BBa_J23100 -1
38/9 BBa_J23100 -2
4NC

Igku Aug10electrophoresis1.jpg

Liquid Culture

Nakamoto

Samplemedium
8/9 BBa-J23106LB(+Amp)
8/9 BBa-J23100-1LB(+Amp)
8/9 BBa-J23100-2LB(+Amp)
8/9 BBa-J44000-1LB(+Amp)
8/9 BBa-J44000-2LB(+Amp)

LB Medium Plate(+Ampicillin)

Nakamoto & Tatsui

volume200ml
Bacto T2ypton 2g
Bacto yeast extract 1g
NaCl1g
0.4M NaOHaq500µL
Agarose Pouder2g
Ampicillin40µL

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
tetR3µL19µL22µLKan
RBS3µL19µL22µLAmp
lacZα3µL19µL22µLCP
lacI3µL19µL22µLCP
Plac3µL19µL22µLCP
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