Team:UNITN-Trento/Notebook/Labposts/08/43

From 2013.igem.org

(Difference between revisions)

Latest revision as of 09:15, 3 October 2013

{ "date" : "2013-08-15", "author" : "Michele-Viola", "title" : "Ferragosto in the lab... Impossibile!!", "content" : "Today we continued to work on Bacillus. The plates that we did yesterday were not so good so we decided to wait for another day to obtain a better growth. However we did a stock of competent Bacillus by starting from inocula did yesterday. These inocula were diluited in new and fresh transforamtion medium and then, when they reached OD about 0.9, glycerol was added and aliquote of 1 ml were done. So if everything will go as expected, finally we have obtained a stock of Mr Bacillus already competent. ", "tags" : "Bacillus" }

@@ Line 1: / Line 1: @@
 {
-	"date" : "2013-08-31",
+	"date" : "2013-08-15",
-	"author" : "fabio",
+	"author" : "Michele-Viola",
-	"title" : " <html> EFE produced upon blue light illumination!!that’s totally awesome!!</html> ",
+	"title" : "Ferragosto in the lab... Impossibile!!",
-	"content" : " <html> in these last days we got our two final complete circuits!! Both my cloning and pedro’s succeeded so now we have a circuit that produces amilCP+EFE with blue light, and a circuit that produces amilGFP+EFE in the dark. We took some gas cromatografic measurements after an evaluated induction time with our LED to see if the devices actually produces ethylene. We could only see a really huge peak) ethylene in the sample under blue light with the final circuit with inverter. So the circuit with inverter seems to work, at least usually. We could not observe ethylene with the circuit that I cloned though.</html> ",
+	"content" : "<html>Today we continued to work on Bacillus. The plates that we did yesterday were not so good so we decided to wait for another day to obtain a better growth. However we did a stock of competent Bacillus by starting from inocula did yesterday. These inocula were diluited in new and fresh transforamtion medium and then, when they reached OD about 0.9, glycerol was added and aliquote of 1 ml were done. So if everything will go as expected, finally we have obtained a stock of Mr Bacillus already competent.   </html>",
-	"tags" : "blue_light"
+	"tags" : "Bacillus"
 }