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<h3>Project Safety</h3>

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        <li><a href=#rsafety>1. Researcher safety</a> </li>

        <li><a href=#labsafety>2. Laboratory safety</a> </li>

        <li><a href=#pubsafety>3. Public safety </a></li>

        <li><a href=#envsafety>4. Environmental safety</a></li>

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    The materials used in our project pose no safety or health concerns to members in the laboratory. In our experiments we have only two kinds of harmful reagents, both used in SDS-PAGE:<br/><br/>

TEMED(N,N,N',N'-Tetramethylethylenediamine): harmful to neural system<br/><br/>

Acrylamide: strongly carcinogenic<br/><br/>

<h4 id=envsafety>Environmental safety</h4>

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  We only used nonpathogenic Escherichia. Coli strains (DH5α, MG1655 ,BL21 and BL21(DE3)) as host bacterias, which all are biosafety level 1 organisms in our project. However, these Escherichia. Coli strains (DH5α, MG1655 ,BL21 and BL21(DE3)) can survive departing from the laboratory. If leaked by accident, our biobricks and genetic materials would do harm to the environment owing to the resistance of Escherichia. Coli to a certain antibiotic even three various antibotics including three different plasmids. To avoid these potential biological pollution to environment, we gathered all culture fluid and culture medium with bacterias with unified treatment ensured that none of them leaked from our laboratory through sewer and dustbin. We handled all the biological materials, wastes and equipment strictly following the BSL-1 requirements. All these things that have physical contacted with biological samples were bleached by autoclave before discarded or reused.<br/><br/>

  In a word, we've taken safety, including researchers safety, lab safety and public safety into accounts. The rules and safety standards we've consulted are pervasive in Xiamen University as well as in China.<br/><br/></p>

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<h3> Biosafety Standards and Institutions</h3>

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  Xiamen University has its own biosafety rules that are regulated by the Ministry of Environment Protection, P.R. China and our institution. We carried out our experiments and evaluated bio-safety issues in our iGEM project strictly following the Chinese Pathogenic Microbiology Laboratory.<br/><br/></p>

<p>Link to the Nation Biosafety Office: </p>

<p><a href=http://english.biosafety.gov.cn/>National Biosafety Office </a></p>

<p>Link to the regulations: </p>

<p><a href=http://www1.www.gov.cn/zwgk/2005-05/23/content_256.htm>Biosafety regulation on pathogenic microbes </a>[2] Bio-safety Regulations, 2004, P.R. China.</p>

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<h3>Biosafety in outreach</h3>

<span> BioBang</span>

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</div><p>In our outreach, in other words, human practice, we designed a special prize for these high school students who were the top ten on our scoring list. These sets of cards-we called them Bio Bang-were inspired by War Within Three Kingdoms, which are very popular in China. There are abundant role-relationship and mutual promotion and restraint between every cards, just like Bong! in America. The Bio Bang is themed with synthetic biology, including genetic engineering experiments, biological materials and equipment. We've also assimilate knowledge of biosafety into these set of cards in order to popularize knowledge of biosafety in lab, public and environment. One example of them related to biosafety are shown as follows:

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<h3>Supplementary</h3>

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