Team:BostonU/Results

From 2013.igem.org

(Difference between revisions)
 
(39 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Template:BU2013}}
 +
<html>
<html>
-
<head>
+
<br><br>
-
<title>BostonU iGEM Team: Welcome</title>
+
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
+
-
<script src="http://cdn.jquerytools.org/1.2.5/full/jquery.tools.min.js?foo"></script>
+
-
<style type="text/css">
+
-
/*hide default igem banner and reformat style into blank slate*/
+
-
#globalWrapper {width: 100%;}
+
-
#top-section {width: 100%; height:30px; border:none;}
+
-
#p-logo {display:none;}
+
-
#search-controls {display:none;}
+
-
#menubar a {color:#000000;}
+
-
#menubar a:hover{text-decoration:none; color:#52749C;}
+
-
.left-menu {background-color:#FFFFFF; margin:0px 0px 0px 0px; padding:0;}
+
-
.left-menu ul {background-color:#FFFFFF; margin:0; padding:0;}
+
-
.right-menu ul li a {background-color:#FFFFFF;}
+
-
.printfooter {display:none;}
+
-
#footer-box {border:none;}
+
-
#catlinks {display:none;}
+
-
.firstHeading {display:none;}
+
-
#content {width: 100%; border:none;}
+
-
#bodyContent {border:none;}
+
-
 
+
-
/*actual content styles*/
+
-
body {width: 800px; margin:auto;}
+
-
 
+
-
#bu-wellesley_wiki_content {height:auto; line-height:100%;}
+
-
/*#bu-wellesley_wiki_content a {color:#69d01d;}*/
+
-
#bu-wellesley_wiki_content a:hover {text-decoration:none; color:#3d3f3c;}
+
-
 
+
-
.navbar li {color: #ffffff;}
+
-
.navbar li a {color: #ffffff;}
+
-
.navbar li a:hover {background:#69d01d; color: #ffffff;}
+
-
 
+
-
/*only use for current page content header (i.e. Team, G-nomeSurferPro, etc)*/
+
-
 
+
-
H4 {
+
-
      font-family: Signika;
+
-
      text-transform: uppercase;
+
-
      text-decoration: none;
+
-
      text-align: left;
+
-
      color: #B22222;
+
-
      font-size: 20pt;
+
-
    }
+
-
 
+
-
H3 {
+
-
      font-family: Signika;
+
-
      text-transform: none;
+
-
      text-decoration: none;
+
-
      text-align: left;
+
-
      color: #696969;
+
-
      font-size: 12pt;
+
-
    }
+
-
H7 {
+
-
      font-family: Signika;
+
-
      text-transform: none;
+
-
      text-decoration: none;
+
-
      text-align: left;
+
-
      color: #000000;
+
-
      font-size: 12pt;
+
-
    }
+
-
 
+
-
H8 {
+
-
      font-family: Signika;
+
-
      text-transform: none;
+
-
      text-decoration: none;
+
-
      text-align: left;
+
-
      color: #B22222;
+
-
      font-size: 12pt;
+
-
    }
+
-
 
+
-
H6 {
+
-
      font-family: Signika;
+
-
      text-transform: none;
+
-
      text-decoration: none;
+
-
      text-align: left;
+
-
      color: #B22222;
+
-
      font-size: 13pt;
+
-
          font-width: 300;
+
-
    }
+
-
 
+
-
H9 {
+
-
      font-family: Signika;
+
-
      text-transform: none;
+
-
      text-decoration: none;
+
-
      text-align: left;
+
-
      color: #B22222;
+
-
      font-size: 14pt;
+
-
    }
+
-
 
+
-
 
+
-
</style>
+
-
<link rel="stylesheet" type="text/css" href="http://static.tumblr.com/bepcnvc/YKnm67emn/team.css">
+
<h2>Results Summary</h2>
-
<link href='http://fonts.googleapis.com/css?family=Signika:300,400' rel='stylesheet' type='text/css'>
+
<body>
 +
<h7><p>Below is a brief summary of our results from this summer. Each statement has a link shown in </h7><h8>RED</h8><h7> to the page(s) containing more information and detailed results. </h7> </p>
 +
<br><br>
 +
<ul>
-
<style type="text/css">@import "http://cs.wellesley.edu/~hcilab/iGEM_wiki/css/videobox.css";</style>
+
<div><table>
 +
<tr><td><img src="https://static.igem.org/mediawiki/2013/f/f1/Updated_Moclo.gif" width="200px"></td>
 +
<td><h7><ul>
-
</head>
+
<br><p>This summer, we created 63 new Level 0 Parts (32 prom, 12 5'UTR, 16 CDS, and 3 terminators), 14 new Destination Vectors, and 86 new Level 1 Transcriptional Units to the <a href="https://2013.igem.org/Team:BostonU/Parts">MoClo Library</a></p>
-
<body class="basiclayout">
+
<p>We have also updated the <a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a> to include 90 Level 0 Basic Parts and 21 Destination Vectors so teams next year can create devices containing up to 4 transcriptional units</p>
-
<div id="bu-wellesley_wiki_content">
+
</ul></td></tr></table></div></ul>
-
<div id="igemlogo">
 
-
<a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/1/13/Igemwhite_back.png" alt="iGEM 2012 main page" style="position: absolute; top: 10px; left: 10px;"width="90"></a>
 
-
</div>
 
-
<p  style="text-align:center;"><a href="https://2013.igem.org/Team:BostonU"><img src="https://static.igem.org/mediawiki/2013/2/2c/Marsh.png" width="800px" length="200px"></a></p>
+
<br>
 +
<ul>
-
<ul id="nav">  
+
<p><div><table>
-
<li><a href="#">Our Team</a>  
+
<tr><td><img src="https://static.igem.org/mediawiki/2013/d/d8/Char_data_BU.gif" width="200px"></td>
-
<ul>
+
<td><h7><ul>
-
<li><a href="https://2013.igem.org/Team:BostonU/Team">Team</a></li>
+
<br><p>Using a state of the art BD LSRFortessa at the Center of Synthetic Biology here at Boston University, we have successfully <a href="https://2013.igem.org/Team:BostonU/Data">characterized</a> 64 Level 1 Transcriptional Units and 2 Level 2 Devices</p>
-
<li><a href="https://2013.igem.org/Team:BostonU/Social">Summer Fun</a></li>
+
<p>From this work, we will draft a proposal for a standardized flow cytometry protocol for future teams to utilize when testing devices containing fluorescent proteins in <i>E. coli</i></p>
-
</ul>
+
-
</li>
+
-
<li><a href="#">Project</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
+
-
                        <li><a href="https://2013.igem.org/Team:BostonU/MoCloChara">Introduction to MoClo and Characterization</a></li>
+
-
                        <ul>
+
-
                            <li><a href="https://2013.igem.org/Team:BostonU/QS">Quorum Sensing</a></li>
+
-
                            <li><a href="https://2013.igem.org/Team:BostonU/HK">Histidine Kinase</a></li>
+
-
                        </ul>
+
-
</li>
+
-
                        <li><a href="https://2013.igem.org/Team:BostonU/DataSheet">Data Sheet App</a></li> 
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Results ">Results Summary</a></li>
+
-
</ul>
+
-
</li>
+
-
<li><a href="#">Achievements</a>
+
-
<ul>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Data">Data Collected</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>  
+
-
</ul>
+
</ul></td></tr></table></div></p></ul>
-
</li>
+
-
<li><a href="#">Notebook</a>
+
<br>
-
<ul>
+
<ul>
-
<li><a href="https://2013.igem.org/Team:BostonU/Protocols">Protocols</a></li>
+
-
                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookML">Characterization Notebook</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/NotebookQS">Quorum Sensing Notebook</a></li>
+
-
                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookHK">Histidine Kinase Notebook</a></li>
+
-
<li><a href="https://2013.igem.org/Team:BostonU/Clotho">Clotho Notebook</a></li>
+
-
        <li><a href="https://2013.igem.org/Team:BostonU/PUP">Puppeteer Notebook</a></li>
+
-
                        <li><a href="https://2013.igem.org/Team:BostonU/TroubleShooting">Troubleshooting</a></li>
+
-
</ul>
+
-
</li>
+
-
+
-
<li><a href="#">Considerations</a>  
+
<p><div><table>
-
<ul>
+
<tr><td><img src="https://static.igem.org/mediawiki/2013/4/49/Data_sheet_tool.gif" width="200px"></td>
-
<li><a href="https://2013.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
+
<td><h7><ul>
-
<li><a href="https://2013.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
+
<br><p>Working closely with the <a href="https://2013.igem.org/Team:Purdue">Purdue Biomakers</a> team, we are designing a <a href="https://2013.igem.org/Team:BostonU/DataSheet">datasheet</a> that will allow users to more easily share information and data within the iGEM community and beyond</p>
-
<li><a href="https://2013.igem.org/Team:BostonU/Safety">Safety</a></li>
+
<p>We have begun implementing a Java-based web tool to generate these data sheets</p>
-
</ul>
+
</ul></td></tr></table></div></p></ul>
-
<li><a href="#">Acknowledgements</a>  
+
<br><br><br>
-
<ul>
+
</body>
-
                    <li><a href="https://2013.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
+
-
                    <li><a href="https://2013.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
+
-
                </ul>
+
</html>
-
</li>
+

Latest revision as of 03:29, 28 September 2013



Results Summary

Below is a brief summary of our results from this summer. Each statement has a link shown in RED to the page(s) containing more information and detailed results.




      This summer, we created 63 new Level 0 Parts (32 prom, 12 5'UTR, 16 CDS, and 3 terminators), 14 new Destination Vectors, and 86 new Level 1 Transcriptional Units to the MoClo Library

      We have also updated the MoClo Kit to include 90 Level 0 Basic Parts and 21 Destination Vectors so teams next year can create devices containing up to 4 transcriptional units



      Using a state of the art BD LSRFortessa at the Center of Synthetic Biology here at Boston University, we have successfully characterized 64 Level 1 Transcriptional Units and 2 Level 2 Devices

      From this work, we will draft a proposal for a standardized flow cytometry protocol for future teams to utilize when testing devices containing fluorescent proteins in E. coli



      Working closely with the Purdue Biomakers team, we are designing a datasheet that will allow users to more easily share information and data within the iGEM community and beyond

      We have begun implementing a Java-based web tool to generate these data sheets