Team:USP-Brazil/Results

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<p style="padding-top: 50px; margin-left: -40px;"><img src="https://static.igem.org/mediawiki/2013/d/d1/TitleUSPBrResults.png" width="117" height="47" alt="Results" /></p>
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<h2>Results</h2>
 
<h3>Overview</h3>
<h3>Overview</h3>
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<p>Besides designing a bioengineered microorganism that could help solve the methanol poisoning  
<p>Besides designing a bioengineered microorganism that could help solve the methanol poisoning  
problem, the pragmatically goal on wet lab was to generate new data on P<sub>AOX1</sub> and P<sub>FLD1</sub>  
problem, the pragmatically goal on wet lab was to generate new data on P<sub>AOX1</sub> and P<sub>FLD1</sub>  
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<li>How strong are the AOX1 and FLD1 promoters?</li>
<li>How strong are the AOX1 and FLD1 promoters?</li>
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<li>How relatively strong are the PAOX1 variants?</li>
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<li>How relatively strong are the P<sub>AOX1</sub> variants?</li>
<li>How different alcohol sources will behave on interaction with wild P<sub>AOX1</sub> and variants? (Activation? Repression?)</li>
<li>How different alcohol sources will behave on interaction with wild P<sub>AOX1</sub> and variants? (Activation? Repression?)</li>
<li>Will P<sub>FLD1</sub> be activated or repressed by inputs other than mentioned on literature (methylamine and methanol)?</li>
<li>Will P<sub>FLD1</sub> be activated or repressed by inputs other than mentioned on literature (methylamine and methanol)?</li>
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<li>Have our <i>Pichia</i> codon-optimized RFP a better fluorescence than a non-optimized (BBa_E1010)?</li>
<li>Have our <i>Pichia</i> codon-optimized RFP a better fluorescence than a non-optimized (BBa_E1010)?</li>
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<li>Microbiology Questions
<li>Microbiology Questions
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<p>Our team managed to answer some of these questions and is still working to completely deliver all of them with good quality. You may start checking our results on our <a href="https://2013.igem.org/Team:USP-Brazil/Results:Assemblies">Assemblies page</a>.</p>
<p>Our team managed to answer some of these questions and is still working to completely deliver all of them with good quality. You may start checking our results on our <a href="https://2013.igem.org/Team:USP-Brazil/Results:Assemblies">Assemblies page</a>.</p>
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  <span style="float:left"><a href="https://2013.igem.org/Team:USP-Brazil/Modeling">Back to our mathematical models…&#8230;</a>
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  <span style="float:left"><a href="https://2013.igem.org/Team:USP-Brazil/Modeling"><i class="icon-circle-arrow-left"></i> Back to our mathematical models</a>
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</span><a href="https://2013.igem.org/Team:USP-Brazil/HumanPractices">Check out our Human Practices results&#8230;</a>
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</span><a href="https://2013.igem.org/Team:USP-Brazil/HumanPractices">Check out our Human Practices results <i class="icon-circle-arrow-right"></i></a>
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Latest revision as of 01:14, 28 September 2013

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Results

Overview

Besides designing a bioengineered microorganism that could help solve the methanol poisoning problem, the pragmatically goal on wet lab was to generate new data on PAOX1 and PFLD1 transcriptional profiles, measuring the performance of kinetic parameters and input compatibility using the standardization principles of synthetic biology [1]. We also tested some P. pastoris grow and cultivation variables to verify how it would behave on detection conditions and on the lyiophilization (freeze-drying) process.

The main questions regarding on our experimental approach are:

  • BioBrick Characterization Questions
    • How strong are the AOX1 and FLD1 promoters?
    • How relatively strong are the PAOX1 variants?
    • How different alcohol sources will behave on interaction with wild PAOX1 and variants? (Activation? Repression?)
    • Will PFLD1 be activated or repressed by inputs other than mentioned on literature (methylamine and methanol)?
    • How long is the time delay response of these promoters in a system with RFP?
    • Have our Pichia codon-optimized RFP a better fluorescence than a non-optimized (BBa_E1010)?


  • Microbiology Questions
    • Will P. pastoris survive on ethanol solutions?
    • Will P. pastoris survive lyophilization? If survives, will the survival population be large enough to deliver the output signal at naked eye!?


Our team managed to answer some of these questions and is still working to completely deliver all of them with good quality. You may start checking our results on our Assemblies page.

Back to our mathematical models Check out our Human Practices results

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