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Team:Buenos Aires/ res rfpdeg
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| - | = GFP under Arsenite Inducible Promoter | + | = GFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) = |
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| + | ---- | ||
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'''Objective''' | '''Objective''' | ||
| - | Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite | + | Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concentrations. |
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'''Methods''' | '''Methods''' | ||
| - | Several cultures carrying a plasmid that encodes GFP under this promoter(Bba_K1106004) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). | + | Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm). |
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| - | + | [[File:Gfpinduccion.jpg|center|600px|]] | |
| - | + | [[File:Gfpampliacion2.jpg|center|600px|]] | |
| - | + | '''Conclusion''' | |
| - | + | ||
| - | + | ||
| + | The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical arsenite concentrations according to the World Health Organisation. | ||
</div> | </div> | ||
Latest revision as of 22:22, 27 September 2013
GFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004])
Objective
Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concentrations.
Methods
Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).
Results
As shown in the graphics below GFP production increases with the arsenite concentration.
Conclusion
The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical arsenite concentrations according to the World Health Organisation.
