|
|
| (2 intermediate revisions not shown) |
| Line 79: |
Line 79: |
| | | | |
| | <div class="article"> | | <div class="article"> |
| - | <h3 id="c1"><a href="#c1">week1(8/18/2013~8/25/2013)</a></h3>
| |
| - | <div>
| |
| - | <p>
| |
| - | <b>8/22//2013</b><br>
| |
| - | .Extracting the human ferritin plasmid and iron chaperone plasmid<br>
| |
| - | <b>8/23/2013</b><br>
| |
| - | .BBa_I746908 and BBa_I746902 transformation<br>
| |
| - | <b>8/24/2013</b><br>
| |
| - | .Extracting BBa_I746908 and BBa_I746902<br>
| |
| - | <b>8/25/2013</b><br>
| |
| - |
| |
| - | </p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | <div class="article">
| |
| - | <h3 id="c2"><a href="#c2">week2(8/26/2013~9/1/2013)</a></h3>
| |
| - | <div>
| |
| - | <p>
| |
| - | <b>8/26/2013</b><br>
| |
| - |
| |
| - | <b>8/27/2013</b><br>
| |
| - |
| |
| - | <b>8/28/2013</b><br>
| |
| - |
| |
| - | <b>8/29/2013</b><br>
| |
| - | .Gel electrophoresis(ferritin,iron chaperone4-6c,4-14I)<br>
| |
| - | .PCR:(4-6c),ferritin(mid,strong)<br>
| |
| - | .Digestion test:4-6c,ferritin,iron chaperone<br>
| |
| - | .Pre-culture:CRISPRi<br>
| |
| - |
| |
| - |
| |
| - | <b>8/30/2013</b><br>
| |
| - | .Extracting CRISPRi plasmids(fur,fieF,gor,and trxB)<br>
| |
| - | .PCR:ferritin(min,strong),CRISPRi(fur,fieF,for,and trxB)<br>
| |
| - | .Gel electrophresis:PCR products<br>
| |
| - | .Gel extractions:PCR products<br>
| |
| - | <b>8/31/2013</b><br>
| |
| - | .Digestion:ferritin(mid and strong),4-6c<br>
| |
| - |
| |
| - | <b>9/1/2013</b><br>
| |
| - | .FASTR:CRISPRi<br>
| |
| - | .Ligation:ferritin and 4-6c<br>
| |
| - | .Begining editing Wiki<br>
| |
| - |
| |
| - | </p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | <div class="article">
| |
| - | <h3 id="c3"><a href="#c3">week3(9/2/2013~9/7/2013)</a></h3>
| |
| - | <div>
| |
| - | <p>
| |
| - | <b>9/2/2013</b><br>
| |
| - | .Making plates with Broth and methylene blue(MB)<br>
| |
| - | <b>9/3/2013</b><br>
| |
| - | .Making olates with Broth and MB<br>
| |
| - | .Culturing BL21 and shuffle , respectively<br>
| |
| - | <b>9/4/2013</b><br>
| |
| - |
| |
| - | <b>9/5/2013</b><br>
| |
| - |
| |
| - | <b>9/6/2013</b><br>
| |
| - |
| |
| - | <b>9/7/2013</b><br>
| |
| - |
| |
| - | </p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | <div class="article">
| |
| - | <h3 id="c4"><a href="#c4">week4(9/8/2013~9/14/2013)</a></h3>
| |
| - | <div>
| |
| - | <p>
| |
| - |
| |
| - | <b>9/9/2013</b><br>
| |
| - | .Hold a presentation(state of progress)<br>
| |
| - | .<br>
| |
| - | <b>9/10/2013</b><br>
| |
| - |
| |
| - | <b>9/11/2013</b><br>
| |
| - |
| |
| - | <b>9/12/2013</b><br>
| |
| - |
| |
| - | <b>9/13/2013</b><br>
| |
| - |
| |
| - | <b>9/14/2013</b><br>
| |
| - |
| |
| - | </p>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - | <br><br><br><br><br><br><br><br><br><br><br>
| |
| - |
| |
| | | | |
| | | | |
| Line 213: |
Line 109: |
| | | | |
| | <th> | | <th> |
| - | <a href="https://2013.igem.org/Team:Chiba/Project/uptake">Iron Uptake</a> | + | <a href="https://2013.igem.org/Team:Chiba/Assay/uptake">Reprogramming the iron homeostasis</a> |
| | </th> | | </th> |
| | | | |
| | <th> | | <th> |
| - | <a href="https://2013.igem.org/Team:Chiba/Project/store">Iron Storage</a> | + | <a href="https://2013.igem.org/Team:Chiba/Assay/store">Establishment of Iron storage system</a> |
| | </th> | | </th> |
| | | | |
| | <th> | | <th> |
| - | <a href="https://2013.igem.org/Team:Chiba/Project/oxidation">Iron Oxidation</a> | + | <a href="https://2013.igem.org/Team:Chiba/Assay/oxidation">Reprogramming the redox state of the cell</a> |
| | </th> | | </th> |
| | | | |
"
