Team:Cornell/notebook/protocols

From 2013.igem.org

(Difference between revisions)
 
(23 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Cornell/templates/header}}
{{:Team:Cornell/templates/header}}
-
<!-- paulirish.com/2008/conditional-stylesheets-vs-css-hacks-answer-neither/ -->
 
-
<!--[if lt IE 7]> <html class="no-js lt-ie9 lt-ie8 lt-ie7" lang="en"> <![endif]-->
 
-
<!--[if IE 7]>    <html class="no-js lt-ie9 lt-ie8" lang="en"> <![endif]-->
 
-
<!--[if IE 8]>    <html class="no-js lt-ie9" lang="en"> <![endif]-->
 
-
<!--[if gt IE 8]><!-->
 
<html class="no-js" lang="en">
<html class="no-js" lang="en">
-
<!--<![endif]-->
+
<script type="text/javascript">
 +
$(window).load(function() {
 +
$('li.pg-notebook_protocols').addClass('active');
 +
});
 +
</script>
<div class="row main-image">
<div class="row main-image">
<div class="twelve columns">
<div class="twelve columns">
-
<img src="https://static.igem.org/mediawiki/2012/9/93/Swati_and_Claire.jpg">
+
<img src="https://static.igem.org/mediawiki/2013/6/60/Cornell_IMG_4991_small.jpg" style="width:940px; height:450px">
<a class="button radius gallery-button" href="https://2013.igem.org/Team:Cornell/team/gallery">More Photos</a>
<a class="button radius gallery-button" href="https://2013.igem.org/Team:Cornell/team/gallery">More Photos</a>
</div>
</div>
Line 29: Line 28:
<li>
<li>
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
<a href="https://static.igem.org/mediawiki/2012/2/2d/Qiagen_Miniprep.pdf" target="_blank">Qiagen Miniprep</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2013/7/79/Column_Purification.pdf" target="_blank">Column Purification</a>
</li>
</li>
</ul>
</ul>
</div>
</div>
<div class="four columns">
<div class="four columns">
-
<h4>DNA Assessment</h4>
+
<h4>Plasmid Editing</h4>
<ul>
<ul>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
+
<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
+
<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
 +
</li>
 +
<li>
 +
<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
 +
</li>
 +
                                        <li>
 +
<a href="https://static.igem.org/mediawiki/2013/9/94/Gibson_Assembly.pdf" target="_blank">Gibson Assembly</a>
</li>
</li>
-
 
</ul>
</ul>
</div>
</div>
Line 47: Line 57:
<h4>PCR Methods</h4>
<h4>PCR Methods</h4>
<ul>
<ul>
 +
                                        <li>
 +
<a href="https://static.igem.org/mediawiki/2013/3/30/Q5_PCR.pdf" target="_blank">Q5 PCR</a>
 +
</li>
<li>
<li>
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
<a href="https://static.igem.org/mediawiki/2012/2/26/Phusion_PCR.pdf" target="_blank">Phusion PCR</a>
Line 72: Line 85:
</div>
</div>
<div class="four columns">
<div class="four columns">
-
<h4>Plasmid Editing</h4>
+
<h4>DNA Assessment</h4>
<ul>
<ul>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/a/af/Double_Digest.pdf" target="_blank">Double Digest</a>
+
<a href="https://static.igem.org/mediawiki/2012/6/6c/DNA_Gel_Electrophoresis.pdf" target="_blank">DNA Gel Electrophoresis</a>
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/f/fb/Single_Digest.pdf" target="_blank">Single Digest</a>
+
<a href="https://static.igem.org/mediawiki/2012/0/03/Sequencing_Preparation.pdf" target="_blank">Preparation of DNA for Sequencing</a>
</li>
</li>
-
<li>
+
                                        <li>
-
<a href="https://static.igem.org/mediawiki/2012/a/a5/Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a>
+
<a href="https://static.igem.org/mediawiki/2013/5/52/Fluorescence_Assay_Procedure.pdf" target="_blank">Fluorescence Assay</a>
-
</li>
+
-
<li>
+
-
<a href="https://static.igem.org/mediawiki/2012/a/a7/T4_Ligase_Ligation.pdf" target="_blank">T4 Ligase Ligation</a>
+
</li>
</li>
 +
</ul>
</ul>
</div>
</div>
Line 91: Line 102:
<h4>Fungal Transformation</h4>
<h4>Fungal Transformation</h4>
<ul>
<ul>
-
<li>
+
<li>
-
<a href="https://static.igem.org/mediawiki/2012/6/6c/Growth_Assay_Protocol.pdf" target="_blank"><i>Shewanella</i> Growth Assay Protocol</a>
+
<a href="https://static.igem.org/mediawiki/2013/5/50/Preparation_of_Protoplasts.pdf" target="_blank">Preparation of Protoplasts</a>
</li>
</li>
<li>
<li>
-
<a href="https://static.igem.org/mediawiki/2012/b/bc/M4_recipe.pdf" target="_blank">M4 Recipe</a>
+
<a href="https://static.igem.org/mediawiki/2013/f/f0/Transformation_of_Protoplasts.pdf" target="_blank">Transformation of Protoplasts</a>
</li>
</li>
-
<li>
+
                                        <li>
-
<a href="https://static.igem.org/mediawiki/2012/c/cb/Synthetic_River_Media.pdf" target="_blank">Synthetic River Media Recipe</a>
+
<a href="https://static.igem.org/mediawiki/2013/9/91/Agrobacteria_Mediated_Transformation_Protocol.pdf " target="_blank">Agrobacteria Mediated Transformation</a>
-
</li>
+
-
<li>
+
-
<a href="https://2012.igem.org/Team:Cornell/protocols/secret_dylan_dance" target="_blank">Plate Drying</a>
+
</li>
</li>
</ul>
</ul>
</div>
</div>
</div>
</div>
-
 
-
<script src="https://2012.igem.org/Team:Cornell/javascripts/foundation.min?action=raw&amp;ctype=text/javascript"></script>
 
-
<script src="https://2012.igem.org/Team:Cornell/javascripts/app?action=raw&amp;ctype=text/javascript"></script>
 
-
<script type="text/javascript">
 
-
$(window).load(function() {
 
-
$('li.pg-protocols').addClass('active');
 
-
});
 
-
</script>
 
</body>
</body>
</html>
</html>

Latest revision as of 03:44, 29 October 2013

Cornell University Genetically Engineered Machines

More Photos

DNA Purification

Plasmid Editing

PCR Methods

Plasmid Insertion

DNA Assessment

Fungal Transformation

Retrieved from "http://2013.igem.org/Team:Cornell/notebook/protocols"