Team:MIT/ResultsBiobricks

From 2013.igem.org

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document.title = "MIT iGEM - BioBricks";
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<div id="col_center">
 
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Biobricks
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<div id="col_left">
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Favorite Parts
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• Name: CMV_Acyl-TyA-eGFP MammoBlock
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o ID: BBa_K1179071
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o Type: Generator
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o Length: 2834
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o Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.
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• Name: Hef1a_Cas9-VP16 MammoBlock
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<div class="section" id="b1bio">
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o ID: BBa_K1179002
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<h1>Favorite Parts:</h1>
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o Type: Generator
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<h3>CMV_Acyl-TyA-eGFP MammoBlock</h3>
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o Length: 5141
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<ul>
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o Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with Cas9 binding sites upstream.
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<li>ID: <a href="http://parts.igem.org/Part:BBa_K1179071">BBa_K1179071</a></li>
 +
<li>Type: Generator</li>
 +
<li>Length: 2834</li>
 +
<li>Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.</li>
 +
</ul>
 +
<h3>hEF1a_Cas9-VP16 MammoBlock</h3>
 +
<ul>
 +
<li>ID: <a href="http://parts.igem.org/Part:BBa_K1179002">BBa_K1179002</a></li>
 +
<li>Type: Generator</li>
 +
<li>Length: 5141</li>
 +
<li>Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.</li>
 +
</ul>
 +
<h3>hEF1a_eYFP-4x451 MammoBlock</h3>
 +
<ul>
 +
<li>ID: <a href="http://parts.igem.org/Part:BBa_K1179072">BBa_K1179072</a></li>
 +
<li>Type: Generator</li>
 +
<li>Length: 2085</li>
 +
<li>Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.</li>
 +
</ul>
 +
</div><!-- end section -->
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• Name: Hef1a_eYFP-4x451 MammoBlock
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<div class="section" id="b2bio">
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o ID: BBa_K1179072
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<h1>All Parts</h1>
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o Type: Generator
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<table border="1" style="margin-top:5px" >
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o Length: 2085
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<tr><td>Acyl-TyA</td><td><a href="http://parts.igem.org/Part:BBa_K1179000">BBa_K1179000</a></td></tr>
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o Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.
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<tr><td>Acyl-TyA-eGFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179001">BBa_K1179001</a></td></tr>
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Other parts we like:
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<tr><td>hEF1a_Cas9-VP16</td><td><a href="http://parts.igem.org/Part:BBa_K1179002">BBa_K1179002</a></td></tr>
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Acyl-TyA BBa_K1179000
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<tr><td>Acyl-TyA-mKate-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179003">BBa_K1179003</a></td></tr>
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Acyl-TyA-eGFP BBa_K1179001
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<tr><td>Acyl-TyA-rtTA</td><td><a href="http://parts.igem.org/Part:BBa_K1179004">BBa_K1179004
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Hef1a_Cas9-VP16 BBa_K1179002
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</a></td></tr>
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Acyl-TyA-mKate-His BBa_K1179003
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<tr><td>Acyl-TyA-rtTA-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179005">BBa_K1179005
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Acyl-TyA-rtTA BBa_K1179004
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</a></td></tr>
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Acyl-TyA-rtTA-His BBa_K1179005
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<tr><td>Acyl-TyA-Tev</td><td><a href="http://parts.igem.org/Part:BBa_K1179006">BBa_K1179006
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Acyl-TyA-Tev BBa_K1179006
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</a></td></tr>
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Acyl-TyA-TEV-rtTA-His BBa_K1179007
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<tr><td>Acyl-TyA-TEV-rtTA-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179007">BBa_K1179007
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Cas9 D10A & H840A mutation-linker BBa_K1179008
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</a></td></tr>
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Cas9-Linker-VP16 BBa_K1179009
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<tr><td>Cas9 D10A & H840A mutation-linker</td><td><a href="http://parts.igem.org/Part:BBa_K1179008">BBa_K1179008
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eBFP BBa_K1179010
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</a></td></tr>
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eYFP BBa_K1179012
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<tr><td>Cas9-Linker-VP16</td><td><a href="http://parts.igem.org/Part:BBa_K1179009">BBa_K1179009
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eYFP-PEST BBa_K1179013
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</a></td></tr>
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VP16-GAL4 BBa_K1179014
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<tr><td>eBFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179010">BBa_K1179010
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U6_gRNA (Cr9) BBa_K1179015
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</a></td></tr>
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L7Ae BBa_K1179016
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<tr><td>eYFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179012">BBa_K1179012
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Linker-Cas9 D10A & H840A mutation BBa_K1179017
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</a></td></tr>
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mKate –PEST BBa_K1179018
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<tr><td>eYFP-PEST</td><td><a href="http://parts.igem.org/Part:BBa_K1179013">BBa_K1179013
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mKate-His BBa_K1179019
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</a></td></tr>
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rtTA-His BBa_K1179020
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<tr><td>VP16-GAL4</td><td><a href="http://parts.igem.org/Part:BBa_K1179014">BBa_K1179014
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CAG_Acyl-TyA-mKate-His BBa_K1179021
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</a></td></tr>
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CAG_Acyl-TyA-rtTA BBa_K1179022
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<tr><td>U6_gRNA (Cr9)</td><td><a href="http://parts.igem.org/Part:BBa_K1179015">BBa_K1179015
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CAG_Acyl-TyA-rtTA-His BBa_K1179023
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</a></td></tr>
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CAG_eYFP-PEST BBa_K1179024
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<tr><td>L7Ae</td><td><a href="http://parts.igem.org/Part:BBa_K1179016">BBa_K1179016
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CAG_L7Ae BBa_K1179025
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</a></td></tr>
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CAG_mKate-PEST BBa_K1179026
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<tr><td>Linker-Cas9 D10A & H840A mutation</td><td><a href="http://parts.igem.org/Part:BBa_K1179017">BBa_K1179017
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CAG-tagBFP BBa_K1179027
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</a></td></tr>
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D10A and H840A mutant Cas9-VC155 BBa_K1179028
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<tr><td>mKate –PEST</td><td><a href="http://parts.igem.org/Part:BBa_K1179018">BBa_K1179018
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D10A and H840A mutant Cas9-VN155 BBa_K1179029
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</a></td></tr>
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D10A and H840A mutant Cas9-VN173 BBa_K1179030
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<tr><td>mKate-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179019">BBa_K1179019
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CMV_ L7Ae_myc-His6 BBa_K1179032
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</a></td></tr>
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CMV_2xKMet-eGFP BBa_K1179033
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<tr><td>rtTA-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179020">BBa_K1179020
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L7Ae-2a-eYFP BBa_K1179037
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</a></td></tr>
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TRE_Cas9-VP16 BBa_K1179040
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<tr><td>CAG_Acyl-TyA-mKate-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179021">BBa_K1179021
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U6_gRNA 1A Reverse BBa_K1179041
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</a></td></tr>
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U6_gRNA 1A BBa_K1179042
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<tr><td>CAG_Acyl-TyA-rtTA</td><td><a href="http://parts.igem.org/Part:BBa_K1179022">BBa_K1179022
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U6_gRNA 2A BBa_K1179043
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</a></td></tr>
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U6_gRNA 3A BBa_K1179044
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<tr><td>CAG_Acyl-TyA-rtTA-His</td><td><a href="http://parts.igem.org/Part:BBa_K1179023">BBa_K1179023
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U6_gRNA 4A BBa_K1179045
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</a></td></tr>
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U6_gRNA 5A BBa_K1179046
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<tr><td>CAG_eYFP-PEST</td><td><a href="http://parts.igem.org/Part:BBa_K1179024">BBa_K1179024
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U6_gRNA 6A BBa_K1179047
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</a></td></tr>
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U6_gRNA 6A Reverse BBa_K1179048
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<tr><td>CAG_L7Ae</td><td><a href="http://parts.igem.org/Part:BBa_K1179025">BBa_K1179025
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VC155 BBa_K1179049
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</a></td></tr>
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VC155 with Leucine zipper BBa_K1179050
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<tr><td>CAG_mKate-PEST</td><td><a href="http://parts.igem.org/Part:BBa_K1179026">BBa_K1179026
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VC155-Cas9(D10A and H840A mutant) BBa_K1179051
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</a></td></tr>
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VN155 BBa_K1179052
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<tr><td>CAG-tagBFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179027">BBa_K1179027
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VN155 with Leucine zipper BBa_K1179053
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</a></td></tr>
-
VN155-Cas9(D10A and H840A mutant) BBa_K1179054
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<tr><td>D10A and H840A mutant Cas9-VC155</td><td><a href="http://parts.igem.org/Part:BBa_K1179028">BBa_K1179028
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VN173 BBa_K1179055
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</a></td></tr>
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VN173 with Leucine zipper BBa_K1179056
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<tr><td>D10A and H840A mutant Cas9-VN155</td><td><a href="http://parts.igem.org/Part:BBa_K1179029">BBa_K1179029
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VN173-Cas9(D10A and H840A mutant) BBa_K1179057
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</a></td></tr>
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Cre entry vector BBa_K1179058
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<tr><td>D10A and H840A mutant Cas9-VN173</td><td><a href="http://parts.igem.org/Part:BBa_K1179030">BBa_K1179030
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eYFP-4x451ts BBa_K1179060
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</a></td></tr>
-
eYFP-4x503ts BBa_K1179061
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<tr><td>CMV_ L7Ae_myc-His6</td><td><a href="http://parts.igem.org/Part:BBa_K1179032">BBa_K1179032
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LacI BBa_K1179062
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</a></td></tr>
-
LacI-4x451ts BBa_K1179063
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<tr><td>CMV_2xKMet-eGFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179033">BBa_K1179033
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LacI-4x503ts BBa_K1179064
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</a></td></tr>
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TagBFP BBa_K1179068
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<tr><td>L7Ae-2a-eYFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179037">BBa_K1179037
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CAG BBa_K1179069
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</a></td></tr>
-
CMV_Acyl-TyA-eGFP BBa_K1179071
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<tr><td>TRE_Cas9-VP16</td><td><a href="http://parts.igem.org/Part:BBa_K1179040">BBa_K1179040
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Hef1a_eYFP-4x451 BBa_K1179072
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</a></td></tr>
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TRE-tight_eYFP-4x451 BBa_K1179073
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<tr><td>U6_gRNA 1A Reverse</td><td><a href="http://parts.igem.org/Part:BBa_K1179041">BBa_K1179041
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Hef1a_eYFP-4x503 BBa_K1179074
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</a></td></tr>
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TRE-t_eYFP-4x503 BBa_K1179075
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<tr><td>U6_gRNA 1A</td><td><a href="http://parts.igem.org/Part:BBa_K1179042">BBa_K1179042
 +
</a></td></tr>
 +
<tr><td>U6_gRNA 2A</td><td><a href="http://parts.igem.org/Part:BBa_K1179043">BBa_K1179043
 +
</a></td></tr>
 +
<tr><td>U6_gRNA 3A</td><td><a href="http://parts.igem.org/Part:BBa_K1179044">BBa_K1179044
 +
</a></td></tr>
 +
<tr><td>U6_gRNA 4A</td><td><a href="http://parts.igem.org/Part:BBa_K1179045">BBa_K1179045
 +
</a></td></tr>
 +
<tr><td>U6_gRNA 5A</td><td><a href="http://parts.igem.org/Part:BBa_K1179046">BBa_K1179046
 +
</a></td></tr>
 +
<tr><td>U6_gRNA 6A</td><td><a href="http://parts.igem.org/Part:BBa_K1179047">BBa_K1179047
 +
</a></td></tr>
 +
<tr><td>U6_gRNA 6A Reverse</td><td><a href="http://parts.igem.org/Part:BBa_K1179048">BBa_K1179048
 +
</a></td></tr>
 +
<tr><td>VC155</td><td><a href="http://parts.igem.org/Part:BBa_K1179049">BBa_K1179049
 +
</a></td></tr>
 +
<tr><td>VC155 with Leucine zipper</td><td><a href="http://parts.igem.org/Part:BBa_K1179050">BBa_K1179050
 +
</a></td></tr>
 +
<tr><td>VC155-Cas9(D10A and H840A mutant)</td><td><a href="http://parts.igem.org/Part:BBa_K1179051">BBa_K1179051
 +
</a></td></tr>
 +
<tr><td>VN155</td><td><a href="http://parts.igem.org/Part:BBa_K1179052">BBa_K1179052
 +
</a></td></tr>
 +
<tr><td>VN155 with Leucine zipper</td><td><a href="http://parts.igem.org/Part:BBa_K1179053">BBa_K1179053
 +
</a></td></tr>
 +
<tr><td>VN155-Cas9(D10A and H840A mutant)</td><td><a href="http://parts.igem.org/Part:BBa_K1179054">BBa_K1179054
 +
</a></td></tr>
 +
<tr><td>VN173</td><td><a href="http://parts.igem.org/Part:BBa_K1179055">BBa_K1179055
 +
</a></td></tr>
 +
<tr><td>VN173 with Leucine zipper</td><td><a href="http://parts.igem.org/Part:BBa_K1179056">BBa_K1179056
 +
</a></td></tr>
 +
<tr><td>VN173-Cas9(D10A and H840A mutant)</td><td><a href="http://parts.igem.org/Part:BBa_K1179057">BBa_K1179057
 +
</a></td></tr>
 +
<tr><td>Cre entry vector</td><td><a href="http://parts.igem.org/Part:BBa_K1179058">BBa_K1179058
 +
</a></td></tr>
 +
<tr><td>eYFP-4x451ts</td><td><a href="http://parts.igem.org/Part:BBa_K1179060">BBa_K1179060
 +
</a></td></tr>
 +
<tr><td>eYFP-4x503ts</td><td><a href="http://parts.igem.org/Part:BBa_K1179061">BBa_K1179061
 +
</a></td></tr>
 +
<tr><td>LacI</td><td><a href="http://parts.igem.org/Part:BBa_K1179062">BBa_K1179062
 +
</a></td></tr>
 +
<tr><td>LacI-4x451ts</td><td><a href="http://parts.igem.org/Part:BBa_K1179063">BBa_K1179063
 +
</a></td></tr>
 +
<tr><td>LacI-4x503ts</td><td><a href="http://parts.igem.org/Part:BBa_K1179064">BBa_K1179064
 +
</a></td></tr>
 +
<tr><td>TagBFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179068">BBa_K1179068
 +
</a></td></tr>
 +
<tr><td>CAG</td><td><a href="http://parts.igem.org/Part:BBa_K1179069">BBa_K1179069
 +
</a></td></tr>
 +
<tr><td>CMV_Acyl-TyA-eGFP</td><td><a href="http://parts.igem.org/Part:BBa_K1179071">BBa_K1179071
 +
</a></td></tr>
 +
<tr><td>hEF1a_eYFP-4x451</td><td><a href="http://parts.igem.org/Part:BBa_K1179072">BBa_K1179072
 +
</a></td></tr>
 +
<tr><td>TRE-tight_eYFP-4x451</td><td><a href="http://parts.igem.org/Part:BBa_K1179073">BBa_K1179073
 +
</a></td></tr>
 +
<tr><td>hEF1a_eYFP-4x503</td><td><a href="http://parts.igem.org/Part:BBa_K1179074">BBa_K1179074
 +
</a></td></tr>
 +
<tr><td>TRE-t_eYFP-4x503</td><td><a href="http://parts.igem.org/Part:BBa_K1179075">BBa_K1179075</a>
 +
</table>
 +
</div> <!--End section-->
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Latest revision as of 20:07, 28 October 2013

iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

Favorite Parts:

CMV_Acyl-TyA-eGFP MammoBlock

  • ID: BBa_K1179071
  • Type: Generator
  • Length: 2834
  • Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.

hEF1a_Cas9-VP16 MammoBlock

  • ID: BBa_K1179002
  • Type: Generator
  • Length: 5141
  • Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.

hEF1a_eYFP-4x451 MammoBlock

  • ID: BBa_K1179072
  • Type: Generator
  • Length: 2085
  • Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.

All Parts

Acyl-TyABBa_K1179000
Acyl-TyA-eGFPBBa_K1179001
hEF1a_Cas9-VP16BBa_K1179002
Acyl-TyA-mKate-HisBBa_K1179003
Acyl-TyA-rtTABBa_K1179004
Acyl-TyA-rtTA-HisBBa_K1179005
Acyl-TyA-TevBBa_K1179006
Acyl-TyA-TEV-rtTA-HisBBa_K1179007
Cas9 D10A & H840A mutation-linkerBBa_K1179008
Cas9-Linker-VP16BBa_K1179009
eBFPBBa_K1179010
eYFPBBa_K1179012
eYFP-PESTBBa_K1179013
VP16-GAL4BBa_K1179014
U6_gRNA (Cr9)BBa_K1179015
L7AeBBa_K1179016
Linker-Cas9 D10A & H840A mutationBBa_K1179017
mKate –PESTBBa_K1179018
mKate-HisBBa_K1179019
rtTA-HisBBa_K1179020
CAG_Acyl-TyA-mKate-HisBBa_K1179021
CAG_Acyl-TyA-rtTABBa_K1179022
CAG_Acyl-TyA-rtTA-HisBBa_K1179023
CAG_eYFP-PESTBBa_K1179024
CAG_L7AeBBa_K1179025
CAG_mKate-PESTBBa_K1179026
CAG-tagBFPBBa_K1179027
D10A and H840A mutant Cas9-VC155BBa_K1179028
D10A and H840A mutant Cas9-VN155BBa_K1179029
D10A and H840A mutant Cas9-VN173BBa_K1179030
CMV_ L7Ae_myc-His6BBa_K1179032
CMV_2xKMet-eGFPBBa_K1179033
L7Ae-2a-eYFPBBa_K1179037
TRE_Cas9-VP16BBa_K1179040
U6_gRNA 1A ReverseBBa_K1179041
U6_gRNA 1ABBa_K1179042
U6_gRNA 2ABBa_K1179043
U6_gRNA 3ABBa_K1179044
U6_gRNA 4ABBa_K1179045
U6_gRNA 5ABBa_K1179046
U6_gRNA 6ABBa_K1179047
U6_gRNA 6A ReverseBBa_K1179048
VC155BBa_K1179049
VC155 with Leucine zipperBBa_K1179050
VC155-Cas9(D10A and H840A mutant)BBa_K1179051
VN155BBa_K1179052
VN155 with Leucine zipperBBa_K1179053
VN155-Cas9(D10A and H840A mutant)BBa_K1179054
VN173BBa_K1179055
VN173 with Leucine zipperBBa_K1179056
VN173-Cas9(D10A and H840A mutant)BBa_K1179057
Cre entry vectorBBa_K1179058
eYFP-4x451tsBBa_K1179060
eYFP-4x503tsBBa_K1179061
LacIBBa_K1179062
LacI-4x451tsBBa_K1179063
LacI-4x503tsBBa_K1179064
TagBFPBBa_K1179068
CAGBBa_K1179069
CMV_Acyl-TyA-eGFPBBa_K1179071
hEF1a_eYFP-4x451BBa_K1179072
TRE-tight_eYFP-4x451BBa_K1179073
hEF1a_eYFP-4x503BBa_K1179074
TRE-t_eYFP-4x503BBa_K1179075

Retrieved from "http://2013.igem.org/Team:MIT/ResultsBiobricks"