"
Team:MIT/Safety
From 2013.igem.org
| (5 intermediate revisions not shown) | |||
| Line 14: | Line 14: | ||
<h1>Safety</h1> | <h1>Safety</h1> | ||
<p> | <p> | ||
| - | <h> | + | <h><li><b>''Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?''<h></b> |
| - | <p>Our team is working on exosome-mediated cell-to-cell communication in mammalian cells. All of our team worked in a BL2 lab with E. coli, and a about half the team also team worked in an adjoining BL2 tissue culture facility with Human Embryonic Kidney cells and Jurkat T Cels. These cell lines are uncontaminated, and we follow standard BL2 protocols to ensure our safety. Potentially hazardous chemicals, such as dimethyl sulfoxide (DMSO), are handled according to recommendations listed on materials safety data sheets (MSDS). The team participated in 4 hours of classroom training administered by MIT Environmental Health and Safety and was closely monitored by a MIT EHS representative during all lab work. We were in compliance with local and national safety and lab-specific equipment regulations to avoid potential cross-contamination. All researchers donned personal protective equipment including gloves, laboratory coats, and eyewear when necessary.<p> | + | <p>Our team is working on exosome-mediated cell-to-cell communication in mammalian cells. All of our team worked in a BL2 lab with E. coli, and a about half the team also team worked in an adjoining BL2 tissue culture facility with Human Embryonic Kidney cells and Jurkat T Cels. These cell lines are uncontaminated, and we follow standard BL2 protocols to ensure our safety. Potentially hazardous chemicals, such as dimethyl sulfoxide (DMSO), are handled according to recommendations listed on materials safety data sheets (MSDS). The team participated in 4 hours of classroom training administered by MIT Environmental Health and Safety and was closely monitored by a MIT EHS representative during all lab work. We were in compliance with local and national safety and lab-specific equipment regulations to avoid potential cross-contamination. All researchers donned personal protective equipment including gloves, laboratory coats, and eyewear when necessary. The number of team members working with mammalian cells was small in size and each individual was specifically trained by MIT EHS and adhered to the national and local safety protocols for mammalian interaction. Extreme care was taken so that there was no cross contamination between labs through the use of equipment and protective gear specified for mammalian work only.<p> |
| - | <h> | + | <h><li><b>''Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?''</b><h> |
<p>We have developed many BioBricks this summer. However, none of our parts have potential hazards for any organisms. We made the decision not to pursue viral infection as a means of delivery of nucleic acids to mammalian cells, ensuring our risk was fully minimized. Additionally, no killer proteins or any protein known to trigger a serious immune response in researchers or other organisms were used. We encourage other teams to follow our lead -- utilizing transient lipid transfection methods instead of viral infections and look forward to interacting with all mammalian-oriented research teams.<p> | <p>We have developed many BioBricks this summer. However, none of our parts have potential hazards for any organisms. We made the decision not to pursue viral infection as a means of delivery of nucleic acids to mammalian cells, ensuring our risk was fully minimized. Additionally, no killer proteins or any protein known to trigger a serious immune response in researchers or other organisms were used. We encourage other teams to follow our lead -- utilizing transient lipid transfection methods instead of viral infections and look forward to interacting with all mammalian-oriented research teams.<p> | ||
| - | <h> | + | <h><li><b>''Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?''</b><h> |
<p>At MIT the Environmental Health and Safety (EHS) office serves as our biosafety committee for all of the labs on campus. An EHS representative for the Weiss Lab approves each student’s biosafety and lab-specific training. All students receive BL2 lab training and students working with mammalian cells received additional blood-borne pathogen training. EHS training includes General Biosafety for Researchers, Managing Hazardous Waste, General Chemical Hygiene and lab-specific training. We have consistently been in contact with our EHS representative, Carolyn Stahl, who is very supportive of the team’s efforts. Throughout the summer she regularly visited the lab space to ensure we were still working to follow biosafety guidelines.<p> | <p>At MIT the Environmental Health and Safety (EHS) office serves as our biosafety committee for all of the labs on campus. An EHS representative for the Weiss Lab approves each student’s biosafety and lab-specific training. All students receive BL2 lab training and students working with mammalian cells received additional blood-borne pathogen training. EHS training includes General Biosafety for Researchers, Managing Hazardous Waste, General Chemical Hygiene and lab-specific training. We have consistently been in contact with our EHS representative, Carolyn Stahl, who is very supportive of the team’s efforts. Throughout the summer she regularly visited the lab space to ensure we were still working to follow biosafety guidelines.<p> | ||
| - | <h> | + | <h><li><b>''Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?''</b> |
<p>We mainly used the mammoblock standard to create our parts, which allowed for safe storage of these parts. Since the mammoblocks use bacterial entry vectors, the mammalian parts can be stored in a BL1 setting, facilitating safer shipping and submission of parts. We hope that when future iGEM teams work with mammalian cells, they use the mammoblock standard to allow for coordinated and safer contribution of parts. Additionally, we rely on safe methods of nucleic acid delivery -- ensuring other teams will easily be able to transition to mammalian work in a safe manner.<p> | <p>We mainly used the mammoblock standard to create our parts, which allowed for safe storage of these parts. Since the mammoblocks use bacterial entry vectors, the mammalian parts can be stored in a BL1 setting, facilitating safer shipping and submission of parts. We hope that when future iGEM teams work with mammalian cells, they use the mammoblock standard to allow for coordinated and safer contribution of parts. Additionally, we rely on safe methods of nucleic acid delivery -- ensuring other teams will easily be able to transition to mammalian work in a safe manner.<p> | ||
</p> | </p> | ||
| - | + | <div align="center"> | |
| + | <tr><td><img src="http://i.imgur.com/Trrb2O7.jpg" width="50%" /></td> | ||
</div><!--end block-content--> | </div><!--end block-content--> | ||
</div> <!--End col_center--> | </div> <!--End col_center--> | ||
Latest revision as of 00:33, 29 October 2013
Safety
Our team is working on exosome-mediated cell-to-cell communication in mammalian cells. All of our team worked in a BL2 lab with E. coli, and a about half the team also team worked in an adjoining BL2 tissue culture facility with Human Embryonic Kidney cells and Jurkat T Cels. These cell lines are uncontaminated, and we follow standard BL2 protocols to ensure our safety. Potentially hazardous chemicals, such as dimethyl sulfoxide (DMSO), are handled according to recommendations listed on materials safety data sheets (MSDS). The team participated in 4 hours of classroom training administered by MIT Environmental Health and Safety and was closely monitored by a MIT EHS representative during all lab work. We were in compliance with local and national safety and lab-specific equipment regulations to avoid potential cross-contamination. All researchers donned personal protective equipment including gloves, laboratory coats, and eyewear when necessary. The number of team members working with mammalian cells was small in size and each individual was specifically trained by MIT EHS and adhered to the national and local safety protocols for mammalian interaction. Extreme care was taken so that there was no cross contamination between labs through the use of equipment and protective gear specified for mammalian work only.
We have developed many BioBricks this summer. However, none of our parts have potential hazards for any organisms. We made the decision not to pursue viral infection as a means of delivery of nucleic acids to mammalian cells, ensuring our risk was fully minimized. Additionally, no killer proteins or any protein known to trigger a serious immune response in researchers or other organisms were used. We encourage other teams to follow our lead -- utilizing transient lipid transfection methods instead of viral infections and look forward to interacting with all mammalian-oriented research teams.
At MIT the Environmental Health and Safety (EHS) office serves as our biosafety committee for all of the labs on campus. An EHS representative for the Weiss Lab approves each student’s biosafety and lab-specific training. All students receive BL2 lab training and students working with mammalian cells received additional blood-borne pathogen training. EHS training includes General Biosafety for Researchers, Managing Hazardous Waste, General Chemical Hygiene and lab-specific training. We have consistently been in contact with our EHS representative, Carolyn Stahl, who is very supportive of the team’s efforts. Throughout the summer she regularly visited the lab space to ensure we were still working to follow biosafety guidelines.
We mainly used the mammoblock standard to create our parts, which allowed for safe storage of these parts. Since the mammoblocks use bacterial entry vectors, the mammalian parts can be stored in a BL1 setting, facilitating safer shipping and submission of parts. We hope that when future iGEM teams work with mammalian cells, they use the mammoblock standard to allow for coordinated and safer contribution of parts. Additionally, we rely on safe methods of nucleic acid delivery -- ensuring other teams will easily be able to transition to mammalian work in a safe manner.
