Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 4th September.html

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Latest revision as of 18:14, 3 October 2013

Trojan Horse

ASDF

4^th September

Results of silencing experiments

It works !

Miller assay

XXXXXX

Redo the silencing experiment

- Launch O/N culture of producing phages cells ; centrifuge ; take supernatant and filter it.

-diluting 100x O/N of MGZ1, F+ -pACYc in LB-Cm (25 ug/ul)

- At OD 0,7 : 5 tubes. Put 900 uL of cells in each tube. Add in tubes respectively : nothing, 100uL phages of litmusGFP-LacZ1, litmusGFP-Cm1 & litmusGFP-Cm2

litmusGFP-lacZ1 & litmusGFP-lacZ2 are used as negative control.

- Incubate 45 minutes at 37°C

- Serial Dilute every tube until 10-5. Plate 100ul for each tube according to this dilution table

	LB	Cm 25 ug/ul	Cm 125 ug/ul	Cm 250ug/ul	Cm 500 ug/ul
non infected	10^-4	10^-4	10^-4	10^-4	10^-4
sRNA anti lacz	10^-4	10^-4	10^-3	10^-3	10^-3
sRNA Cm1	10^-4	10^-4	10^-3	10^-3	10^-3
sRNA Cm2	10^-4	10^-4	10^-3	10^-3	10^-3
sRNA Cm3	10^-4	10^-4	10^-3	10^-3	10^-3
sRNA Cm4	10^-4	10^-4	10^-3	10^-3	10^-3

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-Place your twit here
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+<p><b>Results of silencing experiments</b></p>
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      <em><strong>Miller assay</strong></em>