Team:DTU-Denmark/Notebook/17 September 2013

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== Description ==
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{{:Team:DTU-Denmark/Templates/StartPage|17 September 2013}}
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Navigate to the [[Team:DTU-Denmark/Notebook/16_September_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/18_September_2013|Next]] Entry
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=Lab 223=
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The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically.  Specifically, we measure nitrous oxide production over a period of 10 hours.
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==Main purpose==
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== Methods ==
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The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.
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Protocol [https://2013.igem.org/Team:DTU-Denmark/Methods/Bioreactor Bioreactor]
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==Who was in the lab==
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Helen, Kasia, Ariadni
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[[File:Dtu-bioreactor.jpg|left|thumb|600px|This image shows the bioreactor set up to run in triplicate.]]
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==Procedure==
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== Results ==
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Following the protocol [[Team:DTU-Denmark/Methods/Bioreactor|Bioreactor]].
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==Results==
Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.
Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.
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== Discussion ==
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At the end of the experiment the OD measurements were:
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{| class="wikitable" style="text-align: right"
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!  OD(end)!! sample A!! sample B!! sample C
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|-
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| -|| 0.0255 || 0.1590 || 0.0801
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|-
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|}
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===Colorimetric results===
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'''Ranges'''
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* ''Measuring range 5-150 mg/L NH<sub>4</sub>-N''
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* ''Measuring range 0.02-1 mg/L NO<sub>2</sub>-N''
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{| class="wikitable" style="text-align: right"
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! salt (mg/L)!! sample A/start!! sample A/end!! sample B/start!! sample B/end!! sample C/start!! sample C/end
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|-
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| ammonium  || 18 || 18 || 16 || 6 || 18 ||14
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|-
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| nitrite || 0.93 || 0.91 || 0.92 || 1.72 || 0.9 || 0.9
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|-
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|}
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== Conclusion ==
There were several sources of error in this experiment.  Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.   
There were several sources of error in this experiment.  Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.   
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{{:Team:DTU-Denmark/Templates/EndPage}}
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Latest revision as of 11:00, 4 October 2013

17 September 2013

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Contents

Lab 223

Main purpose

The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.

Who was in the lab

Helen, Kasia, Ariadni

Procedure

Following the protocol Bioreactor.

Results

Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.

Time Replicate 1 Replicate 2 Replicate 3 Control: Distilled Water
14:00 1.84 mg/L 1.64 mg/L 1.60 mg/L N/A
22:00 1.43 mg/L 1.44 mg/L 1.50 mg/L 1.65 mg/L

At the end of the experiment the OD measurements were:

OD(end) sample A sample B sample C
- 0.0255 0.1590 0.0801


Colorimetric results

Ranges

  • Measuring range 5-150 mg/L NH4-N
  • Measuring range 0.02-1 mg/L NO2-N


salt (mg/L) sample A/start sample A/end sample B/start sample B/end sample C/start sample C/end
ammonium 18 18 16 6 18 14
nitrite 0.93 0.91 0.92 1.72 0.9 0.9

Conclusion

There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.


Navigate to the Previous or the Next Entry

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