Team:NTNU-Trondheim/Notebook

From 2013.igem.org

(Difference between revisions)
(→Wednesday 03.07.2013)
m
 
(363 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:NTNU-Trondheim/Templates/Header}}
+
<html xmlns="http://www.w3.org/1999/xhtml"
 +
      xmlns:og="http://ogp.me/ns#"
 +
      xmlns:fb="https://www.facebook.com/2008/fbml">
 +
  <head>
 +
    <title>Trondheim iGEM 2013 </title>
 +
    <meta property="og:title" content="Berkeley iGEM 2011"/>
 +
    <meta property="og:type" content="university"/>
 +
    <meta property="og:description"
 +
          content="Berkeley's 2011 iGEM team wiki."/>
 +
<meta name="description" content="Berkeley iGEM 2011" />
 +
<meta name="keywords" content="igem, berkleley, 2011" />
 +
<link rel="stylesheet" href="https://2011.igem.org/Template:Team:Berkeley/nivocss?action=raw&ctype=text/css" type="text/css"/>
 +
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.3.2/jquery.min.js"></script>
 +
<script type="text/javascript" src="https://2011.igem.org/Template:Team:Berkeley/javascript/javanivoslider?action=raw&ctype=text/javascript"></script>
 +
  </head>
-
{{#calendar: year=2013 | title=Team:NTNU-Trondheim }}
 
 +
<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/2/21/Logo2_NTNU.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
-
<html>
 
-
<style media="screen" type="text/css">
 
-
table.calendar          { margin: 100px; padding: 10px; }
+
<map name="igemmap">
-
table.calendar td      { margin: 100px; padding: 2px; vertical-align: top; }
+
  <area  shape="circle" coords="480,40,40" alt="Mercury" href="https://2011.igem.org" onfocus="this.blur()" />
-
table.month .heading td { padding:2px; background-color:#d4d4d4; color:#aaa; text-align:center; font-size:120%; font-weight:bold; }
+
</map>
-
table.month .dow td    { color:#aaa; text-align:center; font-size:110%; }
+
-
table.month td.today    { background-color:#ddd; }
+
-
table.month td {
+
-
    border: none;
+
-
    margin: 0;
+
-
    padding: 1pt 1.5pt;
+
-
    font-weight: bold;
+
-
    font-size: 8pt;
+
-
    text-align: right;
+
-
    background-color: #eee;
+
-
    }
+
-
table.month a { background:none; padding:0 }
 
-
.day-active { color:#0000cc }
 
-
.day-empty  { color:#cc0000 }
 
-
</style>
 
-
</html>
 
 +
<div class= "layout-1000" >
 +
<div class="row">
 +
<div id='cssmenu'>
 +
<ul>
 +
  <li class='active'><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li>
 +
  <li class='has-sub'><a href='#'><span>Project</span></a>
 +
      <ul>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/novelapproach'><span>A novel approach</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Model'><span>Modelling</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
 +
        <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li>
 +
      </ul>
 +
  </li>
 +
  <li class='has-sub'><a href='#'><span>Technical Stuff</span></a>
 +
      <ul>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Notebook'><span>Notebooks</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Protocols'><span>Protocols</span></a></li>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Primers'><span>Primers</span></a></li>
 +
        <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Safety'><span>Safety</span></a></li>
 +
      </ul>
 +
  </li>
 +
  <li class='has-sub'><a href='#'><span>Team</span></a>
 +
      <ul>
 +
        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Team'><span>Meet the team</span></a></li>
 +
        <li class='last'><a href='https://igem.org/Team.cgi?id=1082'><span>Official Team Profile</span></a></li>
 +
      </ul>
 +
  </li>
 +
  <li class='has-sub'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Outreach'><span>Outreach</span></a>
 +
  </li>
 +
    <li class='has-sub'><a href='#'><span>Judging</span></a>
 +
    <ul>
 +
    <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li>
 +
    <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Medalcriteria'><span>Medal criteria</span></a></li>
 +
    </ul>
 +
    </li>
 +
  <li class='last'><a href='https://2012.igem.org/Team:NTNU_Trondheim/Matchmaker'><span>Matchmaker</span></a></li>
 +
</ul>
 +
</div>
 +
<div> <div class ="row-end"> </div>
 +
</div>
 +
</div>
-
<div id="Default">
 
-
== Notebook ==
 
 +
<div class="row">
 +
<div class="col12" id="spacer3"></div>
 +
<div class="row-end"> </div>
 +
</div>
-
[https://2013.igem.org/Team:NTNU-Trondheim/TransformedBricks Transformed BioBricks]
+
<div class="row">
 +
<div class="col12-2" align = "justify">
 +
<div class="col8" align = "center" style="background-color:#C3A7F3;>
 +
<p style="text-align:center; color:black; "> Notebook</p> </div>
 +
<div class ="row-end"> </div>
 +
</div>
-
====Tuesday 18.06.13====
+
<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/June">June</a><br>
-
 
+
<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/July">July</a><br>
-
 
+
<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/August">August</a><br>
-
Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts Promoter+RBS+RFP+term (<partinfo>BBa_I13521</partinfo>), Promoter+RBS+GFP+term (<partinfo>BBa_I13522</partinfo>), Promoter+RBS+CFP+term (<partinfo>BBa_I13600</partinfo>) and P<sub>m</sub>+RBS+mCherry+term to competent ''E.coli'' DH5&alpha; cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
+
<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/September">September</a><br>
-
 
+
<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/October">October</a><br>
-
For our transformation protocol, see the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols protocol page].
+
<br></br>
-
----
+
<a href="https://2013.igem.org/Team:NTNU-Trondheim/TransformedBricks">Transformed Biobrick</a><br>
-
 
+
-
====Wednesday 19.06.13====
+
-
 
+
-
We transformed BioBricks consisting of fluorescent proteins. Transformed the parts YFP (<partinfo>BBa_E0030</partinfo>), CFP (<partinfo>BBa_E0020</partinfo>), RFP (<partinfo>BBa_E1010</partinfo>), GFP (<partinfo>BBa_E0040</partinfo>), BFP (<partinfo>BBa_K592100</partinfo>) and SYFP (<partinfo>BBa_K864100</partinfo>).
+
-
 
+
-
We transferred mCherry from agar plate to liquid medium and incubated at 37&deg; C.
+
-
----
+
-
 
+
-
====Thursday 20.06.13====
+
-
 
+
-
We transferred the transformed cells from yesterday to liquid medium and incubated at 37&deg;C [https://2013.igem.org/Team:NTNU-Trondheim/Protocols protocol].
+
-
 
+
-
We isolated the DNA from the transformed mCherry cells by using the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols miniprep protocol] and measured the concentration by [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer].
+
-
 
+
-
{|border="1"
+
-
|Sample
+
-
|Concentration (ng/&micro;l)
+
-
|-
+
-
|mCherry1
+
-
|5,6
+
-
|-
+
-
|mCherry2
+
-
|7,3
+
-
|}
+
-
----
+
-
 
+
-
====Friday 21.06.13====
+
-
 
+
-
We isolated the DNA from the transformed cells by using the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols miniprep protocol] and measured the concentration by [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer].
+
-
 
+
-
{|border="1"
+
-
|Sample
+
-
|Concentration (ng/&micro;l)
+
-
|-
+
-
|CFP1
+
-
|8,59
+
-
|-
+
-
|CFP2
+
-
|7,04
+
-
|-
+
-
|BFP
+
-
|7,06
+
-
|-
+
-
|SYFP
+
-
|5,42
+
-
|-
+
-
|RFP1
+
-
|5,04
+
-
|-
+
-
|RFP2
+
-
|7,52
+
-
|-
+
-
|YFP
+
-
|6,79
+
-
|-
+
-
|GFP
+
-
|5,27
+
-
|}
+
-
----
+
-
 
+
-
====Monday 24.06.13 - Friday 28.06.13====
+
-
 
+
-
This week we have established protocols for further work and designed primers for PCR.
+
-
----
+
-
 
+
-
====Monday 01.07.13====
+
-
 
+
-
We transformed some BioBricks: pBAD promoter (<partinfo>BBa_K206000</partinfo>), RBS (<partinfo>BBa_J61101</partinfo>) and a plasmid backbone  (<partinfo>BBa_J01101</partinfo>).
+
-
----
+
-
====Tuesday 02.07.2013====
+
-
 
+
-
This day we started making the small scale vesicle preparation according to our vesicle isolation protocol.
+
-
 
+
-
Overnight cell cultures of ''E.coli'' ER2566 and ''E.coli'' BW27784 transformed with the pUM9 plasmid were preprared. The pUM9 plasmid has amp^R induces a stress respons in ''E.coli'' when arabinose is present. According to the litterature (link?) stress in ''E.coli'' increases vesicle production.
+
-
 
+
-
The ER2566 cell were cultured in plain LB, while the BW27784 cells were cultured in LB with ampenicillin (100 &micro;g/mL).
+
-
 
+
-
We transferred the samples of RBS, pBAD and the plasmid backbone (pTet) from agar plate to liquid medium and incubated at 37&deg; C.
+
-
----
+
-
 
+
-
====Wednesday 03.07.2013====
+
-
 
+
-
The small-scale vesicle preparation continued with completion of step 2-7 in the vesicle isolation protocol. There where made three samples in step 2; ''E.coli'' ER2566 in LB, ''E.coli'' BW27784 in LB with ampenicillin (100 &micro;g/mL) (ara-) and ''E.coli'' BW27784 in LB with ampenicillin (100 &micro;g/mL) with the addition of arabinose (0.5 %) after 1 hour of incubtion (ara+).
+
-
 
+
-
Optical denisty (OD) at 600 nm was mesured as indicated in the table below.
+
-
 
+
-
{|border="l"
+
-
|Sample
+
-
|OD<sub>600</sub>
+
-
|Estimated CFU
+
-
|-
+
-
|ER2566
+
-
|0.9805
+
-
|
+
-
|-
+
-
|ara-
+
-
|0.1263
+
-
|
+
-
|-
+
-
|ara+
+
-
|0.0471
+
-
|
+
-
|}
+
-
 
+
-
 
+
-
We isolated the DNA from the transformed cells (with RBS, pBAD and pTet) by using the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols miniprep protocol] and measured the concentration by [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer].
+
-
 
+
-
{|border="1"
+
-
|Sample
+
-
|Concentration (ng/&micro;l)
+
-
|-
+
-
|RBS
+
-
|4,91
+
-
|-
+
-
|pTet1
+
-
|8,99
+
-
|-
+
-
|pTet2
+
-
|4,39
+
-
|-
+
-
|pBAD1
+
-
|3,54
+
-
|-
+
-
|pBAD2
+
-
|9,24
+
-
|}
+
-
----
+
-
====Thursday 04.07.2013====
+
-
 
+
-
Continuation of the small-scale vesicle preparation according to the vesicle isolation protocol. Step 8-12 was completed with the exception that the pallet was resuspended in 0.5 mL DPBSS insted of 100 &micro;L and that the check for sterility was not performed.
+
</div>
</div>
 +
</html>
-
{{:Team:NTNU-Trondheim/Templates/Footer}}
+
{{Team:NTNU-Trondheim/Press/header}}
 +
{{Team:NTNU-Trondheim/Press/css}}
 +
{{Team:NTNU-Trondheim/Press/navigation}}

Latest revision as of 22:45, 4 October 2013

Trondheim iGEM 2013

header
Mercury
Notebook

June
July
August
September
October


Transformed Biobrick


Retrieved from "http://2013.igem.org/Team:NTNU-Trondheim/Notebook"