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- | '''Taxi.Coli: smart drug delivery''' | + | |
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| + | <br><br><br> |
| + | We are proud to participate in iGEM 2013 and are excited to present<br> |
| + | '''Taxi.Coli'''. |
| + | <br> |
| + | Taxi.Coli is a highly flexible and adaptable smart drug delivery system.<br> |
| + | We want to use E.coli as a ''Taxi'', that expresses streptavidin on its surface as ''Seats'' for the ''Passengers'', which are the biotinylated nanoparticles that contain a drug. Once Taxi.Coli arrives at ''the Address'', a specific environment, it will produce an enzyme that degrades the nanoparticles, releasing the incorporated drug ''the passenger gets out of the car''. <br>Our effort will be limited to a proof of principle, but one can imagine a wide range of future applications. |
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| + | <a href='https://2013.igem.org/Team:EPF_Lausanne/Overview'> |
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| + | Project |
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| + | Team |
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| + | <a href='https://2013.igem.org/Team:EPF_Lausanne/Safety'> |
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| + | Safety |
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| + | <a href='https://2013.igem.org/Team:EPF_Lausanne/Outreach_Overview'> |
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| + | Outreach |
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- | EPF_Lausanne’s team is proud to participate to iGEM 2013 and excited to present their project: Taxi.Coli: smart drug delivery. The team’s vision is to build a biosynthetic drug delivery concept. The key word of this project is “adaptability”. Our goal is to explore a way of using E.Coli as a highly modular carrier, opening the gate to several applications and alternatives in disease treatments. Using the principles of synthetic biology, we engineered a gelatinase secreting E. Coli able to bind gelatin nanoparticles using a biotin-streptavidin interaction and release their cargo in a corresponding location. The drug delivery system is built in three parts: 1) the nanoparticle binding and 2) the environment sensing that 3) triggers the gelatinase release of the engineered E. Coli, liberating the content of the nanoparticle.
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| + | ==Achievements:== |
| + | - We can produce biotinylated nanoparticles<br> |
| + | - We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles<br> |
| + | - We can engineer E.coli to sense different values of pH<br> |
| + | - We can make E.coli express superfolded GFP constitutively<br> |
| + | - We can excite other students about synthetic biology<br> |
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| Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]! | | Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]! |
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- | <!-- Mise en page --> | + | <h2>Medal requirements</h2> |
- | <div style='clear:both'></div> | + | <h3>Bronze medal</h3> |
| + | <ul class="checkmark-list"> |
| + | <li>We registered the team and had fun in the competition.</li> |
| + | <li>We filled in the Judging form.</li> |
| + | <li>We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.</li> |
| + | <li>We plan to present a poster and talk at the iGEM Jamboree in Lyon.</li> |
| + | <li>We designed and submitted the following Biobricks BBa_K1111001, BBa_K1111002, BBa_K111103, BBa_K1111004, BBa_K1111005, BBa_K1111006, BBa_K1111007, BBa_K1111008, BBa_K1111009, BBa_K1111010, BBa_K1111011, BBa_K1111012, BBa_K1111013, BBa_K1111014.</li> |
| + | </ul> |
| + | <h3>Silver medal</h3> |
| + | <ul class="checkmark-list"> |
| + | <li>We experimentally validated that the Biobrick BBa_K1111005 works as expected.</li> |
| + | <li>We documented all our parts on the iGEM Registry of Standard Biological Parts.</li> |
| + | <li>We investigated the safety issues which could be raised by our project. Take a look at our safety page.</li> |
| + | </ul> |
| + | <h3>Gold medal</h3> |
| + | <ul class="checkmark-list"> |
| + | <li>We improved the function of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. This will help future teams to use it.</li> |
| + | <li>We prepared an amazing synthetic biology kit which will be used at public events and high school visits at EPFL. Take a look at our Outreach page. </li> |
| + | <li>We organized a women and engineering's day at our university to promote women in science. </li> |
| + | <br><br> |
| + | <h3>European Jamboree Results</h3> |
| + | <ul class="checkmark-list"> |
| + | <li>SILVER MEDAL</li> |
| + | <li>ADVANCE TO WORLD CHAMPIONSHIP</li> |
| + | </ul> |
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| + | <br> |
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| + | == Thanks to our sponsors == |
| + | Many thanks to our sponsors who made it possible for us to take part in this amazing competition. |
| + | <br> |
| + | [[File:Team-EPF-Lausanne sponso.jpg|center|550px]] |
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