Team:EPF Lausanne/Calendar/6 September 2013

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Sensing <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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'''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP''' <BR>
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I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cylces are 10C° bellow the norma annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, proably in the Primer Mixes. So I did the primer mixes again.
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''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR>
 +
-I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cycles are 10C° bellow the normal annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, probably in the Primer Mixes. So I did the primer mixes again.
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<BR><BR>
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<font size = "4"> Nanoparticles</font> <BR>
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''ELISE-like Assay''<BR>
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We finished the ELISA-like assay and it worked!
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Latest revision as of 22:15, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing-Effector

PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP
-I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cycles are 10C° bellow the normal annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, probably in the Primer Mixes. So I did the primer mixes again.

Nanoparticles

ELISE-like Assay
We finished the ELISA-like assay and it worked!

Wiki Links

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