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Team:Heidelberg/Templates/Del week14 G
From 2013.igem.org
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(→Restriction digest of fragment FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI) |
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==29-07-2013== | ==29-07-2013== | ||
| - | ===Restriction digest of fragment FS_08 to FS_23; 6.5 kb; | + | ===Restriction digest of fragment FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI=== |
| - | [[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|150px|thumb|Test restriction digest (29 | + | [[File:Heidelberg_20130729restrictiondigest 2log FS02-FS03 FS02-FS05 FS08-FS23besch.png|150px|thumb|Test restriction digest (29-07); run at 100 V, 0.8 % gel (TAE)]] |
Incubation at 37°C for about 3 hours | Incubation at 37°C for about 3 hours | ||
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! what !! µl | ! what !! µl | ||
|- | |- | ||
| - | | FS_08 to FS_23 (2) ( | + | | FS_08 to FS_23 (2) (23-07-2013) || 15 |
|- | |- | ||
| ClaI || 1 | | ClaI || 1 | ||
Latest revision as of 18:44, 3 October 2013
29-07-2013
Restriction digest of fragment FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI
Incubation at 37°C for about 3 hours
| what | µl |
|---|---|
| FS_08 to FS_23 (2) (23-07-2013) | 15 |
| ClaI | 1 |
| Buffer CutSmart | 2 |
| dd H2O | 2 |
| Expected fragment lengths [bp] | 3291, 1936, 1303 |
Results:
- Restriction digest of DelG (FS_08 to FS_23) was successful all predicted bands are clearly visible
