Sophierivara (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} {{Template:EPFL2013Footer}}") |
Leabernier (Talk | contribs) |
||
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| + | <font size = "4">Cell Surface Display</font> | ||
| + | |||
| + | ''PCR optimisation'' <BR> | ||
| + | To improve the ''pINP_construct'' amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis. | ||
| + | <BR><BR> | ||
| + | |||
| + | <font size = "4">General</font> | ||
| + | <BR>Competent cells try 3: it worked! | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
PCR optimisation
To improve the pINP_construct amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis.
General
Competent cells try 3: it worked!
"
