Team:EPF Lausanne/Calendar/8 August 2013

From 2013.igem.org

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{{Template:EPFL2013Header}}
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<font size = "4"> Cell Surface Display </font> <BR>
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<font size = "4"> Cell Surface Display </font>  
''Streptavidin iGEM Cloning ''  
''Streptavidin iGEM Cloning ''  
<br> Gradient PCR of Streptavidin iGEM miniprep with the following conditions: 68,70,72 °C ; mix without DMSO and mix with 3% and 5% DMSO.
<br> Gradient PCR of Streptavidin iGEM miniprep with the following conditions: 68,70,72 °C ; mix without DMSO and mix with 3% and 5% DMSO.
<br> Gel electrophoresis to visualise products.
<br> Gel electrophoresis to visualise products.
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<br> The 72°C without DSMO showed least aspecific bands so we decided to go on with the larger amount PCR.
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<br> The 72°C without DSMO showed least unspecific bands so we decided to go on with the larger amount PCR.
<br> larger PCR of 50µl at 72°C without DMSO in the mix.
<br> larger PCR of 50µl at 72°C without DMSO in the mix.
<br> Gel Electrophoresis to verify product.
<br> Gel Electrophoresis to verify product.
<BR>
<BR>
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<font size = "4"> Sensing </font> <BR>
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<font size = "4"> Sensing-Effector</font>  
''Amplifying Plasmids'' <BR>
''Amplifying Plasmids'' <BR>
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Trasformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiemnts.
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Transformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiments.
 +
<BR><BR>
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'''Nanoparticles'''
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<font size = "4"> Nanoparticles</font>  
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<br>- Measurement of the new GPs with the DLS: we got no results.
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<br>- Measurement of the food dye we used diluted in water: we got the same results as for our first measurement; i.e. some 10nm and a few 100nm particles. We can conclude we never had nanoparticles, the protocol we used did not work!
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 +
''Making Nanoparticles, 1st try''
 +
<br>- Measurement of the new GPs with the DLS: we got no results.
 +
<br>- Measurement of the food dye we used diluted in water: we got the same results as for our first measurement; i.e. some 10 nm and a few 100 nm particles. We can conclude we never had nanoparticles, the protocol we used did not work!
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 21:42, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Streptavidin iGEM Cloning
Gradient PCR of Streptavidin iGEM miniprep with the following conditions: 68,70,72 °C ; mix without DMSO and mix with 3% and 5% DMSO.
Gel electrophoresis to visualise products.
The 72°C without DSMO showed least unspecific bands so we decided to go on with the larger amount PCR.
larger PCR of 50µl at 72°C without DMSO in the mix.
Gel Electrophoresis to verify product.


Sensing-Effector

Amplifying Plasmids
Transformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiments.

Nanoparticles

Making Nanoparticles, 1st try
- Measurement of the new GPs with the DLS: we got no results.
- Measurement of the food dye we used diluted in water: we got the same results as for our first measurement; i.e. some 10 nm and a few 100 nm particles. We can conclude we never had nanoparticles, the protocol we used did not work!


Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/8_August_2013"