Team:EPF Lausanne/Calendar/14 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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Sensing <BR>
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''BBa_K523013 Characterisation'' <br>
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Characterisation of the iGEM plasmid BBa_K523013 we decided to use.
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<br><br>
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'''Amplifying Plasmids containing gelatinases''' <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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We transformed two cultures each with a plasmid containing a gene that encoded either MMP2 or MMP9 (two gelatinases).
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'''Streaking Stabculture''' <BR>
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''Amplifying Plasmids containing gelatinases'' <BR>
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Along with the plasmids we recieved a stabculture already transformed with the two enzyme-plasmids. We streaked these cultures on LB+Ampicillin plates and let them grow over night.
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-We transformed two cultures each with a plasmid containing a gene that encoded either MMP2 or MMP9 (two gelatinases).
 +
 
 +
''Streaking Stabculture'' <BR>
 +
-Along with the plasmids we received a stabculture already transformed with the two enzyme-plasmids. We streaked these cultures on LB+Ampicillin plates and let them grow over night.
 +
<BR> <BR>
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<font size = "4"> Nanoparticles</font> <BR>
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''Making Nanoparticles, 2nd try'' <BR>
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-We used a new protocol.  
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'''Nanoparticles'''
 
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Make nanoparticles: try 2. We used a new protocol.
 
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Latest revision as of 21:49, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

BBa_K523013 Characterisation
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Sensing-Effector

Amplifying Plasmids containing gelatinases
-We transformed two cultures each with a plasmid containing a gene that encoded either MMP2 or MMP9 (two gelatinases).

Streaking Stabculture
-Along with the plasmids we received a stabculture already transformed with the two enzyme-plasmids. We streaked these cultures on LB+Ampicillin plates and let them grow over night.

Nanoparticles

Making Nanoparticles, 2nd try
-We used a new protocol.


Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/14_August_2013"