Team:UNITN-Trento/Notebook/Labposts/07/08

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:33, 3 October 2013

{"date":"2013-07-02","author":"viola","title":"EFE+B0015","content":"today i digested B015 in pSB1C3 with the enzymes E and X and EFE in pUC57 with E and S. I started a short cloning protocol with this ligation quantities:

LIGATION	CTRL	1:1	1:2	1:4
BUFFER T4	2 µl	2 µl	2 µl	2 µl
PLASMID (B0015)	2.5 µl	2.5 µl	2.5 µl	2.5 µl
INSEERT (EFE)	0 µl	1.28 µl	2.56 µl	5.12 µl
LIGASE T4	1 µl	1 µl	1 µl	1 µl
H2O	14.5 µl	13.2 µl	11.9 µl	10.6 µl

","tags":"EFE"}

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+{"date":"2013-07-02","author":"viola","title":"<html>EFE+B0015</html>","content":"<html>today i digested B015 in pSB1C3 with the enzymes E and X and EFE in pUC57 with E and S. I started a short cloning protocol with this ligation quantities: <table> <tr> <th>LIGATION</th> <th>CTRL</th> <th>1:1</th> <th>1:2</th> <th>1:4</th> </tr> <tr> <th>BUFFER T4</th> <td>2 µl</td> <td>2 µl</td> <td>2 µl</td> <td>2 µl</td> </tr> <tr> <th>PLASMID (B0015)</th> <td>2.5 µl</td> <td>2.5 µl</td> <td>2.5 µl</td> <td>2.5 µl</td> </tr> <tr> <th>INSEERT (EFE) </th> <td>0 µl</td> <td>1.28 µl</td> <td>2.56 µl</td> <td>5.12 µl</td> </tr> <tr> <th>LIGASE T4 </th> <td>1 µl</td> <td>1 µl</td> <td>1 µl</td> <td>1 µl</td> </tr> <tr> <th>H2O </th> <td>14.5 µl</td> <td>13.2 µl</td> <td>11.9 µl</td> <td>10.6 µl</td> </tr> </table> </html>","tags":"EFE"}
-	"date" : "2013-07-03",
-	"author" : "fabio-viola",
-	"title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
-	"content" : "<html> HORROR!! We lost kind of the whole bacillus that we had, during the night, cause the liquid cultures were disrupted in the shaker at 26!! Luckily we have a survivor, the liquid culture at 37, and all the plates. <br>From the survivor we made further liquid cultures ( at 26°, 1:50 and 1:100; at 37° 1:50 and 1:100) <br>As soon as they became very cloudy we went along with the glycerol stock preparation and put our bacillus at -80°. The one that weren’t cloudy have been kept in the shaker all night long. <br>In the afternoon we inoculated some colonies from the plates in 5 ml of nutrient broth +starch.</html>",
-	"tags" : " Bacillus subtilis"
-}