Team:UNITN-Trento/Notebook/Labposts/07/51

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:44, 3 October 2013

{"date" : "2013-07-22","author" : "emil","title" : "The incompetent Bacillus subtilis saga: The mistaken antibiotic ","content" : "Unfortunately the previous attempt to transform failed maybe due to the mistaken concentration of antibiotic so I restarted generating a stock solution of Kanamicin(10mg/ml).I did an inocula in LB of Bacillus overnight from the stock solution with 50% of glicerol.","tags" : "Bacillus subtilis"}

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+{"date" : "2013-07-22","author" : "emil","title" : "The incompetent Bacillus subtilis saga: The mistaken antibiotic ","content" : "<html>Unfortunately the previous attempt to transform failed maybe due to the mistaken concentration of antibiotic so I restarted generating a stock solution of Kanamicin(10mg/ml).I did an inocula in LB of Bacillus overnight from the stock solution with 50% of glicerol.</html>","tags" : "Bacillus subtilis"}
-	"date" : "2013-07-24",
-	"author" : "fabio-bruno",
-	"title" : " the blue ligation: the never ending story !",
-	"content" : "<html>Today I inoculated 6 colonies of the plated ligation ( 3 from the 1:1 plate  and 2 from the 1:3 plate) <br>I also made the Onetaq Pcr for all the light parts ( blue device,the b sample from the first set; red device, from the third set; and red promoter, from the third set). <br>After that I screened them all and found out that only the blue part was confirmed: up to now we only have the  blue parts confirmed!! Red parts , it seems to me that you’re in hot waters !! <br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Immagine gel|<html><center><img src=\"https://static.igem.org/mediawiki/2013/7/7f/Tn-2013_Daa.jpg\" width=\"450px\" /></center></html>}}<html></html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
-}