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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | <font size = "4">Cell Surface Display</font> | + | <font size = "4">Cell Surface Display</font> <BR> |
Our Primers arrived today. <BR> | Our Primers arrived today. <BR> | ||
''PCR'' <BR> | ''PCR'' <BR> | ||
| - | We did a PCR (see protocols) to | + | We did a PCR (see protocols) to amplify ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. |
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
| - | + | <BR><BR> | |
<font size = "4">General</font> | <font size = "4">General</font> | ||
PCR
We did a PCR (see protocols) to amplify pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
