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''PCR'' <BR> | ''PCR'' <BR> | ||
| - | We did a PCR (see protocols) to | + | We did a PCR (see protocols) to amplify ''pINP_construct'' to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction. |
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised. | ||
<BR><BR> | <BR><BR> | ||
PCR
We did a PCR (see protocols) to amplify pINP_construct to first remove the EYFP sequence and by the same way add overhangs for the Gibson reaction.
We thus did a 0.8% Gel electrophoresis to verify the PCR product. The PCR needed to be optimised.
General
Competent cells try 2.
"
