Team:TU Darmstadt/materials/Media

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<img alt="Problem" src="/wiki/images/6/66/Darmstadt_green_Problem.jpg" width="100" height="30"></a>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook">
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<font size="8" color="#F0F8FF" face="Arial regular">Lab book |</font>
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</a>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/materials">
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<font size="8" color="#F0F8FF" face="Arial regular">Materials |</font>
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<a href="https://2013.igem.org/Team:TU_Darmstadt/protocols">
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<font size="8" color="#F0F8FF" face="Arial regular">Protocols</font>
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<h2><font size="6" color="#F0F8FF" face="Arial regular">Media</font></h2>
<h2><font size="6" color="#F0F8FF" face="Arial regular">Media</font></h2>
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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1.7-DYT (recipe for 500 mL)
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</p></font>
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<ul>
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<li>2.5 g NaCl</li>
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<li>8.0 g tryptone</li>
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<li>5.0 g yeast extract</li>
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<li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li>
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</ul>
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<br>
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<font size="5" color="#F0F8FF" face="Arial regular">
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  <p text-aligne:left style="margin-left:0px; margin-right:50px">
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LB (recipe for 1000 mL)
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</p></font>
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<ul>
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<li>10 g tryptone</li>
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<li>5 g yeast extract</li>
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<li>10 g NaCl</li>
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<li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li>
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<li>for plates: add 15g agar</li>
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</ul>
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SOB (recipe for 1000 mL)
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</p></font>
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<ul>
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<li>0.186 g KCl</li>
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<li>2.4 g MgSO4</li>
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<li>0.584 g NaCl</li>
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<li>20 g tryptone</li>
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<li>5 g yeast extract</li>
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<li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li>
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</ul>
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<br>
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<font size="5" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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SOC (recipe for 1000 mL)
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</p></font>
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<ul>
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<li>980 mL SOB</li>
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<li>20 mL 1M Glucose</li>
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</ul>
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Latest revision as of 00:39, 5 October 2013







Lab book | Materials | Protocols

Media

1.7-DYT (recipe for 500 mL)

  • 2.5 g NaCl
  • 8.0 g tryptone
  • 5.0 g yeast extract
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

LB (recipe for 1000 mL)

  • 10 g tryptone
  • 5 g yeast extract
  • 10 g NaCl
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
  • for plates: add 15g agar

SOB (recipe for 1000 mL)

  • 0.186 g KCl
  • 2.4 g MgSO4
  • 0.584 g NaCl
  • 20 g tryptone
  • 5 g yeast extract
  • add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)

SOC (recipe for 1000 mL)

  • 980 mL SOB
  • 20 mL 1M Glucose

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