Team:TU Darmstadt/materials/Stock solutions

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<a href="https://2013.igem.org/Team:TU_Darmstadt/materials">
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<a href="https://2013.igem.org/Team:TU_Darmstadt/protocols">
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<h2><font size="6" color="#F0F8FF" face="Arial regular">Stock solutions</font></h2>
<h2><font size="6" color="#F0F8FF" face="Arial regular">Stock solutions</font></h2>
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<font size="5" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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Antibiotics
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</p></font>
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<ul>
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<font size="4" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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Ampicillin
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</p></font>
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<ul>
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1.Mix<br>
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<ul>
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<li>4 g ampicillin (100 mg/mL)</li>
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<li>ad. 40 mL dist. water</li>
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</ul>
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2.sterile filtration<br>
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3. aliquot in 1 mL stocks and store at -20°C<br>
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4. use 1µL per 1mL medium<br>
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</ul>
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<br>
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<font size="4" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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Chloramphenicol
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</p></font>
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<ul>
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1.Mix<br>
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<ul>
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<li>1g chloramphenicol</li>
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<li>ad. 40mL ethanol</li>
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</ul>
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2.aliquot in 1mL stocks and store at -20°C<br>
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3. use 1µL per 1mL medium<br><br>
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</ul>
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<font size="4" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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Kanamycin
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</p></font>
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<ul>
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1.Mix<br>
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<ul>
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<li>3 g kanamycin (75 mg/mL) </li>
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<li>ad. 40mL dist. water</li>
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</ul>
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2.sterile filtration<br>
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3.aliquot in 1 mL stocks and store at -20°C<br>
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3. use 1µL per 1mL medium<br><br>
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</ul>
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<br>
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<font size="5" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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Induction chemicals
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IPTG (Isopropyl-beta-D-thiogalactopyranoside)
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</p></font>
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<ul>
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1. Dissolve 238 mg IPTG in 10 mL water<br>
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2. store in 1mL aliquots at -20°C
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</ul>
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<br>
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<font size="5" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:0px; margin-right:50px">
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Loading dye
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</p></font>
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<a href="http://www.thermoscientificbio.com/nucleic-acid-electrophoresis/6x-tritrack-dna-loading-dye/"><font size="3" color="#F0F8FF" face="Arial regular"><b>tritrack loading dye</b></font></a>
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Latest revision as of 03:52, 5 October 2013







Lab book | Materials | Protocols

Stock solutions

Antibiotics

    Ampicillin

      1.Mix
      • 4 g ampicillin (100 mg/mL)
      • ad. 40 mL dist. water
      2.sterile filtration
      3. aliquot in 1 mL stocks and store at -20°C
      4. use 1µL per 1mL medium

    Chloramphenicol

      1.Mix
      • 1g chloramphenicol
      • ad. 40mL ethanol
      2.aliquot in 1mL stocks and store at -20°C
      3. use 1µL per 1mL medium

    Kanamycin

      1.Mix
      • 3 g kanamycin (75 mg/mL)
      • ad. 40mL dist. water
      2.sterile filtration
      3.aliquot in 1 mL stocks and store at -20°C
      3. use 1µL per 1mL medium


    Induction chemicals

    IPTG (Isopropyl-beta-D-thiogalactopyranoside)

      1. Dissolve 238 mg IPTG in 10 mL water
      2. store in 1mL aliquots at -20°C

    Loading dye

    tritrack loading dye

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