Team:Manchester/Notebook
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<li><a href="https://2013.igem.org/Team:Manchester/Modelling" id="link6">Modelling</a> | <li><a href="https://2013.igem.org/Team:Manchester/Modelling" id="link6">Modelling</a> | ||
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- | <li><a href="https://2013.igem.org/Team:Manchester/Enzyme" id="link6"> | + | <li><a href="https://2013.igem.org/Team:Manchester/Enzyme" id="link6">Uncertainty Analysis</a></li> |
<li><a href="https://2013.igem.org/Team:Manchester/FabProteinModel" id="link6">FabA Dynamics Model</a></li> | <li><a href="https://2013.igem.org/Team:Manchester/FabProteinModel" id="link6">FabA Dynamics Model</a></li> | ||
<li><a href="https://2013.igem.org/Team:Manchester/PopulationDynamics" id="link6">Population Dynamics</a></li> | <li><a href="https://2013.igem.org/Team:Manchester/PopulationDynamics" id="link6">Population Dynamics</a></li> | ||
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<li id="two"><a href="" onclick="blocking('text13'); return false;">Week 14</a> | <li id="two"><a href="" onclick="blocking('text13'); return false;">Week 14</a> | ||
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- | <p> | + | <p>This week was filled with frustration, as our gel extractions refused to cooperate with us! Luckily however, Lorna soon became a gel extraction pro, and our DNA yield soon increased to workable levels. </p> |
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- | <p> | + | <p>This week we also ordered our polo shirts, and we got the (correct!) sequencing results back from our fabA gene. </p> |
<p> <center><img src="https://static.igem.org/mediawiki/2013/0/00/Piclab8.jpg" /></center> </p> </div> | <p> <center><img src="https://static.igem.org/mediawiki/2013/0/00/Piclab8.jpg" /></center> </p> </div> | ||
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<p>It’s starting to feel like all of the effort we’ve been putting into the project will pay off! This week we managed to get our genes of interest (fabA, delta 9 and delta 12) into the iGEM submission plasmid! Sequencing showed that our genes are in fact ligated, and so we eagerly arranged a fedEX shipment to send our samples on their merry way to Boston. It’s not over though! We still needed to successfully ligate our genes into an expression plasmid. We chose pSB1C3 with a ribosomal binding site (RBS) and promoter (P) (Part BB1_ K608002). After a few seemingly failed attempts (our colonies were very small and took a while longer than expected to grow), we decided to test digest anyway because what did we have to lose (except all hope)? Excitingly however, the test digestions suggested that we’d put the genes into the expression plasmid! Time caught up with us and so we will be characterising next week. We think that the small colonies may be a result of the constitutive promoter used, and in an ideal world we’d check this theory. With one week to go though we will just have to leave it to any future iGEM teams out there!</p> | <p>It’s starting to feel like all of the effort we’ve been putting into the project will pay off! This week we managed to get our genes of interest (fabA, delta 9 and delta 12) into the iGEM submission plasmid! Sequencing showed that our genes are in fact ligated, and so we eagerly arranged a fedEX shipment to send our samples on their merry way to Boston. It’s not over though! We still needed to successfully ligate our genes into an expression plasmid. We chose pSB1C3 with a ribosomal binding site (RBS) and promoter (P) (Part BB1_ K608002). After a few seemingly failed attempts (our colonies were very small and took a while longer than expected to grow), we decided to test digest anyway because what did we have to lose (except all hope)? Excitingly however, the test digestions suggested that we’d put the genes into the expression plasmid! Time caught up with us and so we will be characterising next week. We think that the small colonies may be a result of the constitutive promoter used, and in an ideal world we’d check this theory. With one week to go though we will just have to leave it to any future iGEM teams out there!</p> | ||
- | <p>The pressure is very much on now, the whole team is feeling it. We’re desperately trying to juggle a chaotic lab schedule, writing up the wiki and making the presentation/poster for the jamboree.</p> | + | <p>The pressure is very much on now, the whole team is feeling it. We’re desperately trying to juggle a chaotic lab schedule, writing up the wiki and making the presentation/poster for the jamboree. However there's still human practices to be done! In addition to all our lab work, we met with a group of local environmentalists and spoke to large industries to aid us in gauging what the public and industry really think about synthetic biology. </p> |
<p> <center><img src="https://static.igem.org/mediawiki/2013/6/60/Piclab14.jpg" /></center> </p> | <p> <center><img src="https://static.igem.org/mediawiki/2013/6/60/Piclab14.jpg" /></center> </p> | ||
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<li id="four"><a href="" onclick="blocking('text15'); return false;">Week 16</a> | <li id="four"><a href="" onclick="blocking('text15'); return false;">Week 16</a> | ||
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- | + | <p>This is it! The final stretch to wikifreeze and the pressure is hotting up. At the start of the week we inoculated our RBS/P and Delta9/Delta12/fabA constructs in FAS media containing the fatty acids we needed to feed the bacteria with. We left our samples in the very capable hands of Dr Rattray who ran our samples on the Orbitrap LC-MS so we could see if our fatty acid profile had changed. The results finally came through the day before wikifreeze, and we were thrilled to find out that our biobricks had worked! </p> | |
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- | + | <p>There’s no time to celebrate though. It’s the day of wiki freeze and we’re furiously working to get our wiki finished. Talk about cutting it fine!</p> | |
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Latest revision as of 17:40, 26 October 2013