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Team:NTNU-Trondheim/Notebook/October
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| - | <div | + | <div id='cssmenu'> |
| - | <ul class= | + | <ul> |
| - | + | <li class='active'><a href='index.html'><span>Home</span></a></li> | |
| - | + | <li class='has-sub'><a href='#'><span>Project</span></a> | |
| - | + | <ul> | |
| - | + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li> | |
| - | + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/novelapproach'><span>A novel approach</span></a></li> | |
| - | + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Model'><span>Modelling</span></a></li> | |
| - | + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li> | |
| - | + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li> | |
| - | + | <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li> | |
| - | <li><a href= | + | </ul> |
| - | + | </li> | |
| + | <li class='has-sub'><a href='#'><span>Technical Stuff</span></a> | ||
| + | <ul> | ||
| + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Notebook'><span>Notebooks</span></a></li> | ||
| + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Protocols'><span>Protocols</span></a></li> | ||
| + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Primers'><span>Primers</span></a></li> | ||
| + | <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Safety'><span>Safety</span></a></li> | ||
| + | </ul> | ||
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| + | <li class='has-sub'><a href='#'><span>Team</span></a> | ||
| + | <ul> | ||
| + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Team'><span>Meet the team</span></a></li> | ||
| + | <li class='last'><a href='https://igem.org/Team.cgi?id=1082'><span>Official Team Profile</span></a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class='has-sub'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Outreach'><span>Outreach</span></a> | ||
| + | </li> | ||
| + | <li class='has-sub'><a href='#'><span>Judging</span></a> | ||
| + | <ul> | ||
| + | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li> | ||
| + | <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Medalcriteria'><span>Medal criteria</span></a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class='last'><a href='https://2012.igem.org/Team:NTNU_Trondheim/Matchmaker'><span>Matchmaker</span></a></li> | ||
</ul> | </ul> | ||
| + | </div> | ||
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| + | <p style="text-align:center; color:black; "> October</p> </div> | ||
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<p style="text-align:center; color:black; "> Wednesday 02.10.2013</p> </div> | <p style="text-align:center; color:black; "> Wednesday 02.10.2013</p> </div> | ||
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Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below | Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below | ||
| - | <div class="col4" style="background-color:white;><a href=" "> <img src=" " width="303"> | + | <div class="col4" style="background-color:white;><a href="https://static.igem.org/mediawiki/2013/8/8e/Bakteri_SDS_gel_2.jpg"> <img src="https://static.igem.org/mediawiki/2013/8/8e/Bakteri_SDS_gel_2.jpg" width="303"> |
| - | <p style="text-align:center; color:black; "> Figure 1.</p> </div> | + | <p style="text-align:center; color:black; "> <b>Figure 1:</b> SDS-PAGE of bacterial samples. From the left: wild type ER2566, tat_GFP_RFP and tat_ProteinG</p> </div> |
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| - | There is evidently produced Protein G in the S3-2B sample as there is an additional band at the expected size of about | + | There is evidently produced Protein G in the S3-2B sample as there is an additional band at the expected size of about 60 kDa. There is also an additional band at the ER1 sample of about 28-30 kDa. This half the expected size of about 60 kDa. This indicates that only RFP is produced in the bacterias.<br></p> |
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| + | <p style="text-align:center; color:black; ">SDS-PAGE of bacterial samples with the tat_GFP_RFP (ER1) and tat_ProteinG construct</p> </div> | ||
| + | <br><br> | ||
| + | <p>A new test on the Pm/XylS promoter was performed with the same conditions as earlier.<br></p> | ||
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| + | <div class="col12-3" align = "center" style="background-color:white;> | ||
| + | <p style="text-align:center; color:black; "> Wednesday 02 - Friday 04.10.2013</p> </div> | ||
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| + | <p> | ||
| + | As the first attempt to send in the tat_GFP_RFP construct to iGEM Headquarters failed, we redid the cloning as we did 26-28.09.2013 and sent in a new construct. | ||
</div> | </div> | ||
Latest revision as of 22:43, 4 October 2013
October
Wednesday 02.10.2013
SDS-PAGE of bacterial samples with the tat_GFP_RFP (ER1) and tat_ProteinG construct
In order to determine if Protein G is produced in the S3-2B sample and wheater a GFP-RFP dimer is produced in the ER1 sample, a SDS-PAGE was performed. Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below
Figure 1: SDS-PAGE of bacterial samples. From the left: wild type ER2566, tat_GFP_RFP and tat_ProteinG
SDS-PAGE of bacterial samples with the tat_GFP_RFP (ER1) and tat_ProteinG construct
A new test on the Pm/XylS promoter was performed with the same conditions as earlier.
Wednesday 02 - Friday 04.10.2013
As the first attempt to send in the tat_GFP_RFP construct to iGEM Headquarters failed, we redid the cloning as we did 26-28.09.2013 and sent in a new construct.
