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Team:NTNU-Trondheim/Primers
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<li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li> | <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col12-2"> | <div class="col12-2"> | ||
| - | <div class="col8" align = "justify" style="background-color:# | + | <div class="col8" align = "justify" style="background-color:#C3A7F3;> |
<p style="text-align:center; color:#1A1719; "> Primers </p> </div> | <p style="text-align:center; color:#1A1719; "> Primers </p> </div> | ||
<br><br> | <br><br> | ||
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<tr> | <tr> | ||
<th>Primer</th> | <th>Primer</th> | ||
| - | <th> | + | <th>Sequence</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<tr> | <tr> | ||
<td><b> F_I_syfp</tr></b></td> | <td><b> F_I_syfp</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b> R_syfp</tr></b></td> | <td><b> R_syfp</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'</td> |
</tr> | </tr> | ||
| + | |||
<tr> | <tr> | ||
<th> BFP</th> | <th> BFP</th> | ||
<tr> | <tr> | ||
<td><b> F_tat_BFP</tr></b></td> | <td><b> F_tat_BFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_I_bfp</tr></b></td> | <td><b>R_I_bfp</tr></b></td> | ||
| - | <td></td> | + | <td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_tat_GFP</tr></b></td> | <td><b>F_tat_GFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_I_gfp</tr></b></td> | <td><b>R_I_gfp</tr></b></td> | ||
| - | <td></td> | + | <td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_I_rfp</tr></b></td> | <td><b>F_I_rfp</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_rfp</tr></b></td> | <td><b>R_pl.b_rfp</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_tat_GFPmut3</tr></b></td> | <td><b>F_tat_GFPmut3</tr></b></td> | ||
| - | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_GFPmut3</tr></b></td> | <td><b>R_pl.b_GFPmut3</tr></b></td> | ||
| - | <td></td> | + | <td>5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_tat_CFP</tr></b></td> | <td><b>F_tat_CFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_l_CFP</tr></b></td> | <td><b>R_l_CFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_l_YFP</tr></b></td> | <td><b>F_l_YFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_YFP</tr></b></td> | <td><b>R_pl.b_YFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_pl.b_PrG</tr></b></td> | <td><b>F_pl.b_PrG</tr></b></td> | ||
| - | <td></td> | + | <td>5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_tat_PrG</tr></b></td> | <td><b>F_tat_PrG</tr></b></td> | ||
| - | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_pl.b_pelB_PrB</tr></b></td> | <td><b>F_pl.b_pelB_PrB</tr></b></td> | ||
| - | <td></td> | + | <td><p>5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC</p> |
| + | <p>gttgactcaccaatcgaagatacccc-3'</p></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_PrG</tr></b></td> | <td><b>R_pl.b_PrG</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_PrGstop</tr></b></td> | <td><b>R_pl.b_PrGstop</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'</td> |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td><b>F_plasmid </tr></b></td> | <td><b>F_plasmid </tr></b></td> | ||
| - | <td></td> | + | <td>5'-ccaggcatcaaataaaacgaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_RFP_pl.b</tr></b></td> | <td><b>F_RFP_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_YFP_pl.b</tr></b></td> | <td><b>F_YFP_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b</tr></b></td> | <td><b>R_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'- ctagtatttctcctctttctctagtagtgc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_tat_pl.b</tr></b></td> | <td><b>R_tat_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <th> | + | <th> Sequencing Primers</th> |
<tr> | <tr> | ||
<td><b>F_sequence</tr></b></td> | <td><b>F_sequence</tr></b></td> | ||
| - | <td></td> | + | <td>5'-tgccacctgacgtctaagaa-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_sequence</tr></b></td> | <td><b>R_sequence</tr></b></td> | ||
| - | <td></td> | + | <td>5'-attaccgcctttgagtgagc-3'</td> |
</tr> | </tr> | ||
Latest revision as of 20:59, 4 October 2013
Primers
| tat-signal sequence | |
|---|---|
| Primer | Sequence |
| F_pl.b_tat | 5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3' |
| F_prefix_tat | 5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3' |
| R_tat | 5'-cgcttgcgccgcagtcgcacgtcg-3' |
| R_GFP_tat | 5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3' |
| R_CFP_tat | 5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3' |
| SYFP | |
| F_I_syfp | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3' |
| R_syfp | 5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3' |
| BFP | |
| F_tat_BFP | 5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' |
| R_I_bfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3' |
| GFP | |
| F_tat_GFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' |
| R_I_gfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3' |
| RFP | |
| F_I_rfp | 5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3' |
| R_pl.b_rfp | 5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3' |
| GFPmut3 | |
| F_tat_GFPmut3 | 5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3' |
| R_pl.b_GFPmut3 | 5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3' |
| CFP | |
| F_tat_CFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3' |
| R_l_CFP | 5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3' |
| YFP | |
| F_l_YFP | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3' |
| R_pl.b_YFP | 5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3' |
| Protein G | |
| F_pl.b_PrG | 5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3' |
| F_tat_PrG | 5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3' |
| F_pl.b_pelB_PrB | 5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC gttgactcaccaatcgaagatacccc-3' |
| R_pl.b_PrG | 5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3' |
| R_pl.b_PrGstop | 5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3' |
| Plasmid backbone | |
| F_plasmid | 5'-ccaggcatcaaataaaacgaaaggc-3' |
| F_RFP_pl.b | 5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3' |
| F_YFP_pl.b | 5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3' |
| R_pl.b | 5'- ctagtatttctcctctttctctagtagtgc-3' |
| R_tat_pl.b | 5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3' |
| Sequencing Primers | |
| F_sequence | 5'-tgccacctgacgtctaagaa-3' |
| R_sequence | 5'-attaccgcctttgagtgagc-3' |
