Team:NTNU-Trondheim/Primers

From 2013.igem.org

(Difference between revisions)

Latest revision as of 20:59, 4 October 2013

Trondheim iGEM 2013

Primers

tat-signal sequence
Primer	Sequence
F_pl.b_tat
5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'
F_prefix_tat
5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'
R_tat
5'-cgcttgcgccgcagtcgcacgtcg-3'
R_GFP_tat
5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'
R_CFP_tat
5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'
SYFP
F_I_syfp
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'
R_syfp
5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'
BFP
F_tat_BFP
5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3'
R_I_bfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'
GFP
F_tat_GFP
5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3'
R_I_gfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'
RFP
F_I_rfp
5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'
R_pl.b_rfp
5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'
GFPmut3
F_tat_GFPmut3
5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'
R_pl.b_GFPmut3
5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'
CFP
F_tat_CFP
5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'
R_l_CFP
5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'
YFP
F_l_YFP
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'
R_pl.b_YFP
5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'
Protein G
F_pl.b_PrG
5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'
F_tat_PrG
5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'
F_pl.b_pelB_PrB
5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC gttgactcaccaatcgaagatacccc-3'
R_pl.b_PrG
5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'
R_pl.b_PrGstop
5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'
Plasmid backbone
F_plasmid
5'-ccaggcatcaaataaaacgaaaggc-3'
F_RFP_pl.b
5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'
F_YFP_pl.b
5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'
R_pl.b
5'- ctagtatttctcctctttctctagtagtgc-3'
R_tat_pl.b
5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'
Sequencing Primers
F_sequence
5'-tgccacctgacgtctaagaa-3'
R_sequence
5'-attaccgcctttgagtgagc-3'

@@ Line 19: / Line 19: @@
-<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/5/55/Logo-ntnu.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
+<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/2/21/Logo2_NTNU.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
@@ Line 33: / Line 33: @@
 <div id='cssmenu'>
 <ul>
-    <li><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li>
+    <li class='active'><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li>
     <li class='has-sub'><a href='#'><span>Project</span></a>
        <ul>
           <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li>
+         <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/novelapproach'><span>A novel approach</span></a></li>
           <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Model'><span>Modelling</span></a></li>
           <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
           <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
-          <li class='last'><a href='https://2013.igem.org/Team:NTNU_Trondheim/Attributions'><span>Attributions</span></a></li>
+          <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li>
        </ul>
     </li>
@@ Line 59: / Line 60: @@
     <li class='has-sub'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Outreach'><span>Outreach</span></a>
     </li>
-   <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li>
+    <li class='has-sub'><a href='#'><span>Judging</span></a>
+     <ul>
+     <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li>
+     <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Medalcriteria'><span>Medal criteria</span></a></li>
+     </ul>
+     </li>
     <li class='last'><a href='https://2012.igem.org/Team:NTNU_Trondheim/Matchmaker'><span>Matchmaker</span></a></li>
 </ul>
-   </div>
+</div>
 <div> <div class ="row-end"> </div>
 </div>
@@ Line 69: / Line 75: @@
 <div class="row">
 <div class="col12-2">
-<div class="col8" align = "justify" style="background-color:#7352B0;>
+<div class="col8" align = "justify" style="background-color:#C3A7F3;>
 <p style="text-align:center; color:#1A1719; "> Primers </p> </div>
 <br><br>
@@ Line 130: / Line 136: @@
 <tr>
 <td><b>F_tat_GFP</tr></b></td>
-<td></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' </td>
 </tr>
 <tr>
 <td><b>R_I_gfp</tr></b></td>
-<td></td>
+<td>5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'</td>
 </tr>
@@ Line 141: / Line 147: @@
 <tr>
 <td><b>F_I_rfp</tr></b></td>
-<td></td>
+<td>5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'</td>
 </tr>
 <tr>
 <td><b>R_pl.b_rfp</tr></b></td>
-<td></td>
+<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'</td>
 </tr>
@@ Line 152: / Line 158: @@
 <tr>
 <td><b>F_tat_GFPmut3</tr></b></td>
-<td></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'</td>
 </tr>
 <tr>
 <td><b>R_pl.b_GFPmut3</tr></b></td>
-<td></td>
+<td>5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'</td>
 </tr>
@@ Line 163: / Line 169: @@
 <tr>
 <td><b>F_tat_CFP</tr></b></td>
-<td></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'</td>
 </tr>
 <tr>
 <td><b>R_l_CFP</tr></b></td>
-<td></td>
+<td>5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'</td>
 </tr>
@@ Line 174: / Line 180: @@
 <tr>
 <td><b>F_l_YFP</tr></b></td>
-<td></td>
+<td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'</td>
 </tr>
 <tr>
 <td><b>R_pl.b_YFP</tr></b></td>
-<td></td>
+<td>5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'</td>
 </tr>
@@ Line 185: / Line 191: @@
 <tr>
 <td><b>F_pl.b_PrG</tr></b></td>
-<td></td>
+<td>5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'</td>
 </tr>
 <tr>
 <td><b>F_tat_PrG</tr></b></td>
-<td></td>
+<td>5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'</td>
 </tr>
 <tr>
 <td><b>F_pl.b_pelB_PrB</tr></b></td>
-<td></td>
+<td><p>5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC</p>
+<p>gttgactcaccaatcgaagatacccc-3'</p></td>
 </tr>
 <tr>
 <td><b>R_pl.b_PrG</tr></b></td>
-<td></td>
+<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'</td>
 </tr>
 <tr>
 <td><b>R_pl.b_PrGstop</tr></b></td>
-<td></td>
+<td>5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'</td>
 </tr>
@@ Line 208: / Line 215: @@
 <tr>
 <td><b>F_plasmid </tr></b></td>
-<td></td>
+<td>5'-ccaggcatcaaataaaacgaaaggc-3'</td>
 </tr>
 <tr>
 <td><b>F_RFP_pl.b</tr></b></td>
-<td></td>
+<td>5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'</td>
 </tr>
 <tr>
 <td><b>F_YFP_pl.b</tr></b></td>
-<td></td>
+<td>5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'</td>
 </tr>
 <tr>
 <td><b>R_pl.b</tr></b></td>
-<td></td>
+<td>5'- ctagtatttctcctctttctctagtagtgc-3'</td>
 </tr>
 <tr>
 <td><b>R_tat_pl.b</tr></b></td>
-<td></td>
+<td>5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'</td>
 </tr>
@@ Line 231: / Line 238: @@
 <tr>
 <td><b>F_sequence</tr></b></td>
-<td></td>
+<td>5'-tgccacctgacgtctaagaa-3'</td>
 </tr>
 <tr>
 <td><b>R_sequence</tr></b></td>
-<td></td>
+<td>5'-attaccgcctttgagtgagc-3'</td>
 </tr>