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Team:TU Darmstadt/materials/Media
From 2013.igem.org
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<a href="https://2013.igem.org/Team:TU_Darmstadt/labbook"> | <a href="https://2013.igem.org/Team:TU_Darmstadt/labbook"> | ||
| - | <font size="8" color="#F0F8FF" face="Arial regular"> | + | <font size="8" color="#F0F8FF" face="Arial regular">Lab book |</font> |
</a> | </a> | ||
<a href="https://2013.igem.org/Team:TU_Darmstadt/materials"> | <a href="https://2013.igem.org/Team:TU_Darmstadt/materials"> | ||
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<p text-aligne:left style="margin-left:50px; margin-right:50px"> | <p text-aligne:left style="margin-left:50px; margin-right:50px"> | ||
| + | <font size="5" color="#F0F8FF" face="Arial regular"> | ||
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
| + | 1.7-DYT (recipe for 500 mL) | ||
| + | </p></font> | ||
<ul> | <ul> | ||
| - | <li> | + | <li>2.5 g NaCl</li> |
| - | <li> | + | <li>8.0 g tryptone</li> |
| - | <li> | + | <li>5.0 g yeast extract</li> |
| + | <li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li> | ||
</ul> | </ul> | ||
| + | <br> | ||
| + | <font size="5" color="#F0F8FF" face="Arial regular"> | ||
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
| + | LB (recipe for 1000 mL) | ||
| + | </p></font> | ||
| + | <ul> | ||
| + | <li>10 g tryptone</li> | ||
| + | <li>5 g yeast extract</li> | ||
| + | <li>10 g NaCl</li> | ||
| + | <li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li> | ||
| + | <li>for plates: add 15g agar</li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | |||
| + | <font size="5" color="#F0F8FF" face="Arial regular"> | ||
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
| + | SOB (recipe for 1000 mL) | ||
| + | </p></font> | ||
| + | <ul> | ||
| + | <li>0.186 g KCl</li> | ||
| + | <li>2.4 g MgSO4</li> | ||
| + | <li>0.584 g NaCl</li> | ||
| + | <li>20 g tryptone</li> | ||
| + | <li>5 g yeast extract</li> | ||
| + | <li>add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)</li> | ||
| + | </ul> | ||
| + | <br> | ||
| + | |||
| + | <font size="5" color="#F0F8FF" face="Arial regular"> | ||
| + | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
| + | SOC (recipe for 1000 mL) | ||
| + | </p></font> | ||
| + | <ul> | ||
| + | <li>980 mL SOB</li> | ||
| + | <li>20 mL 1M Glucose</li> | ||
| + | </ul> | ||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 00:39, 5 October 2013
.jpg){kind=small}
Media
1.7-DYT (recipe for 500 mL)
- 2.5 g NaCl
- 8.0 g tryptone
- 5.0 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
LB (recipe for 1000 mL)
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
- for plates: add 15g agar
SOB (recipe for 1000 mL)
- 0.186 g KCl
- 2.4 g MgSO4
- 0.584 g NaCl
- 20 g tryptone
- 5 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
SOC (recipe for 1000 mL)
- 980 mL SOB
- 20 mL 1M Glucose
