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| - | We are proud to participate in IGEM 2013 and are excited to present<br> | + | <br><br><br> |
| - | '''Taxi.Coli - smart drug delivery''' | + | We are proud to participate in iGEM 2013 and are excited to present<br> |
| - | <br><br>
| + | '''Taxi.Coli'''. |
| - | The basic idea is simple: <br>
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| - | We will use E.coli as a "Taxi", add streptavidin to the surface as "Seats" and nanoparticles incorporating drugs as the "Passengers". The "Taxi" will recognize the right "Adress" with a pH sensor and the "Passengers" gets released by an enzyme. | + | Taxi.Coli is a highly flexible and adaptable smart drug delivery system.<br> |
| - | ==Achievements:==
| + | We want to use E.coli as a ''Taxi'', that expresses streptavidin on its surface as ''Seats'' for the ''Passengers'', which are the biotinylated nanoparticles that contain a drug. Once Taxi.Coli arrives at ''the Address'', a specific environment, it will produce an enzyme that degrades the nanoparticles, releasing the incorporated drug ''the passenger gets out of the car''. <br>Our effort will be limited to a proof of principle, but one can imagine a wide range of future applications. |
| - | - we can produce biotynilated nanoparticles<br>
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| - | - we can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles<br>
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| - | - we can engineer E.coli to sense different levels of pH<br>
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| | + | ==Achievements:== |
| | + | - We can produce biotinylated nanoparticles<br> |
| | + | - We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles<br> |
| | + | - We can engineer E.coli to sense different values of pH<br> |
| | + | - We can make E.coli express superfolded GFP constitutively<br> |
| | + | - We can excite other students about synthetic biology<br> |
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| - | Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]!
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| | + | Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]! |
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| | <h3>Bronze medal</h3> | | <h3>Bronze medal</h3> |
| | <ul class="checkmark-list"> | | <ul class="checkmark-list"> |
| - | <li>Register the team, have fun in the competition.</li> | + | <li>We registered the team and had fun in the competition.</li> |
| - | <li>iGEM 2013 Judging form.</li> | + | <li>We filled in the Judging form.</li> |
| - | <li>Description of the team's project using the iGEM wiki and the team's parts using the Registry of Standard Biological Parts.</li> | + | <li>We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.</li> |
| - | <li>Plan to present a poster and talk at the iGEM Jamboree.</li> | + | <li>We plan to present a poster and talk at the iGEM Jamboree in Lyon.</li> |
| - | <li>Document at least one new standard BioBrick Part and submit it to the Registry + a new application and documentation of a previously existing BioBrick part in the Registry<br> We sent the following Biobricks:<br>BBa_K1111002, BBa_K1111004, BBa_K1111005, BBa_K1111007, BBa_K1111009, BBa_K1111012, BBa_K1111013, BBa_K1111014. <br> </li> | + | <li>We designed and submitted the following Biobricks BBa_K1111001, BBa_K1111002, BBa_K111103, BBa_K1111004, BBa_K1111005, BBa_K1111006, BBa_K1111007, BBa_K1111008, BBa_K1111009, BBa_K1111010, BBa_K1111011, BBa_K1111012, BBa_K1111013, BBa_K1111014.</li> |
| | </ul> | | </ul> |
| | <h3>Silver medal</h3> | | <h3>Silver medal</h3> |
| | <ul class="checkmark-list"> | | <ul class="checkmark-list"> |
| - | <li>Experimentally validate that at least one new BioBrick Part of your own design and construction works as expected. BBa_K1111005</li> | + | <li>We experimentally validated that the Biobrick BBa_K1111005 works as expected.</li> |
| - | <li>Document the characterization of this part and submit this new part to the iGEM Parts Registry</li> | + | <li>We documented all our parts on the iGEM Registry of Standard Biological Parts.</li> |
| - | <li>Implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project. Take a look at our safety page.</li> | + | <li>We investigated the safety issues which could be raised by our project. Take a look at our safety page.</li> |
| | </ul> | | </ul> |
| | <h3>Gold medal</h3> | | <h3>Gold medal</h3> |
| | <ul class="checkmark-list"> | | <ul class="checkmark-list"> |
| - | <li>Improve the function of an existing BioBrick Part or Device <br> We documented and characterized the already existing Biobrick BBa_K523013 extensively </li> | + | <li>We improved the function of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. This will help future teams to use it.</li> |
| - | <li>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation. Take a look at our Outreach page. </li> | + | <li>We prepared an amazing synthetic biology kit which will be used at public events and high school visits at EPFL. Take a look at our Outreach page. </li> |
| | + | <li>We organized a women and engineering's day at our university to promote women in science. </li> |
| | + | <br><br> |
| | + | <h3>European Jamboree Results</h3> |
| | + | <ul class="checkmark-list"> |
| | + | <li>SILVER MEDAL</li> |
| | + | <li>ADVANCE TO WORLD CHAMPIONSHIP</li> |
| | + | </ul> |
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| | == Thanks to our sponsors == | | == Thanks to our sponsors == |
| - | Many thanks to our sponsors who made it possible for us to take part in this amazing competition | + | Many thanks to our sponsors who made it possible for us to take part in this amazing competition. |
| | + | <br> |
| | + | [[File:Team-EPF-Lausanne sponso.jpg|center|550px]] |
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| - | [[Image:Team-EPF-Lausanne-sponsor.jpeg|thumb|left|300px|Figure1: AC IMMUNE]]
| + | <br><br> |
| - | [[Image:Team-EPF-Lausanne-sponsor4.png|thumb|left|220px|Figure1: EPFL]]
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| - | [[Image:Team-EPF-Lausanne-sponsor5.jpeg|thumb|left|220px|Figure1: Life Sciences and Technology Faculty at EPFL]]
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