Team:EPF Lausanne

From 2013.igem.org

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We are proud to participate in iGEM 2013 and are excited to present<br>
We are proud to participate in iGEM 2013 and are excited to present<br>
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'''Taxi.Coli: smart drug delivery'''
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'''Taxi.Coli'''.
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The basic idea is to create a higly flexible and adaptable smart drug delivery system. The biosynthetic system consists of E.coli as a "Taxi", who express streptavidin on the surface as "Seats" for the "Passangers", which are the biotinylated nanoparticles. Once the Taxi.Coli recognizes a certain medium, it will produce an enzyme that degrades the nanoparticles, thus releasing the incorporated drug.  
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Taxi.Coli is a highly flexible and adaptable smart drug delivery system.<br>
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We want to use E.coli as a ''Taxi'', that expresses streptavidin on its surface as ''Seats'' for the ''Passengers'', which are the biotinylated nanoparticles that contain a drug. Once Taxi.Coli arrives at ''the Address'', a specific environment, it will produce an enzyme that degrades the nanoparticles, releasing the incorporated drug ''the passenger gets out of the car''. <br>Our effort will be limited to a proof of principle, but one can imagine a wide range of future applications.
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==Motivation==
 
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Smart drug delivery Taxi.Coli is a highly flexible and adaptable
 
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The team’s vision is to build a biosynthetic drug delivery concept. The key word of this project is “adaptability”. Our goal is to explore a way of using E.coli as a highly modular carrier, opening the gate to several applications and alternatives in disease treatments. Using the principles of synthetic biology, we engineered a gelatinase secreting E.coli able to bind gelatin nanoparticles using a biotin-streptavidin interaction and release their cargo in a corresponding location. The drug delivery system is built in three parts: 1) the nanoparticles binding and 2) the environment sensing that 3) triggers the gelatinase release of the engineered E.coli, liberating the content of the nanoparticle.
 
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==Achievements:==
 
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- We can produce biotinylated nanoparticles<br>
 
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- We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles<br>
 
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- We can engineer E.coli to sense different levels of pH<br>
 
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==Achievements:==
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- We can produce biotinylated nanoparticles<br>
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- We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles<br>
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- We can engineer E.coli to sense different values of pH<br>
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- We can make E.coli express superfolded GFP constitutively<br>
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- We can excite other students about synthetic biology<br>
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Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]!
 
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Please also visit our [https://www.facebook.com/groups/EPFL.IGEM2013/ Facebook page]!
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<h3>Bronze medal</h3>
<h3>Bronze medal</h3>
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   <li>We registerd the team and had fun in the competition.</li>
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   <li>We registered the team and had fun in the competition.</li>
   <li>We filled in the Judging form.</li>
   <li>We filled in the Judging form.</li>
   <li>We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.</li>
   <li>We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.</li>
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<h3>Gold medal</h3>
<h3>Gold medal</h3>
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   <li>We improved the funtion of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. Which will help future teams to use it.</li>
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   <li>We improved the function of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. This will help future teams to use it.</li>
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   <li>We prepared an amazing synthetic biology kit which will be used at public events and highschool visits at EPFL. Take a look at our Outreach page. </li>
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   <li>We prepared an amazing synthetic biology kit which will be used at public events and high school visits at EPFL. Take a look at our Outreach page. </li>
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<li>We organized a women and engineering's day, at our university to promote women in science. </li>
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<li>We organized a women and engineering's day at our university to promote women in science. </li>
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<h3>European Jamboree Results</h3>
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  <li>SILVER MEDAL</li>
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  <li>ADVANCE  TO WORLD CHAMPIONSHIP</li>
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== Thanks to our sponsors ==
== Thanks to our sponsors ==
Many thanks to our sponsors who made it possible for us to take part in this amazing competition.
Many thanks to our sponsors who made it possible for us to take part in this amazing competition.
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[[Image:Team-EPF-Lausanne-sponsor.jpeg|thumb|left|250px|AC IMMUNE]]
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[[Image:Team-EPF-Lausanne-sponsor4.png|thumb|left|220px|EPFL]]
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[[File:Team-EPF-Lausanne sponso.jpg|center|550px]]  
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[[Image:Team-EPF-Lausanne-sponsor5.jpeg|thumb|left|220px|Life Sciences and Technology Faculty at EPFL]]  
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{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Latest revision as of 00:06, 29 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header




We are proud to participate in iGEM 2013 and are excited to present
Taxi.Coli.
Taxi.Coli is a highly flexible and adaptable smart drug delivery system.
We want to use E.coli as a Taxi, that expresses streptavidin on its surface as Seats for the Passengers, which are the biotinylated nanoparticles that contain a drug. Once Taxi.Coli arrives at the Address, a specific environment, it will produce an enzyme that degrades the nanoparticles, releasing the incorporated drug the passenger gets out of the car.
Our effort will be limited to a proof of principle, but one can imagine a wide range of future applications.





Project
Team
Safety
Outreach





Achievements:

- We can produce biotinylated nanoparticles
- We can engineer E.coli expressing streptavidin (maybe even on the surface) to bind these nanoparticles
- We can engineer E.coli to sense different values of pH
- We can make E.coli express superfolded GFP constitutively
- We can excite other students about synthetic biology



Please also visit our Facebook page!


Medal requirements

Bronze medal

  • We registered the team and had fun in the competition.
  • We filled in the Judging form.
  • We described our project using the iGEM wiki and our parts using the Registry of Standard Biological Parts.
  • We plan to present a poster and talk at the iGEM Jamboree in Lyon.
  • We designed and submitted the following Biobricks BBa_K1111001, BBa_K1111002, BBa_K111103, BBa_K1111004, BBa_K1111005, BBa_K1111006, BBa_K1111007, BBa_K1111008, BBa_K1111009, BBa_K1111010, BBa_K1111011, BBa_K1111012, BBa_K1111013, BBa_K1111014.

Silver medal

  • We experimentally validated that the Biobrick BBa_K1111005 works as expected.
  • We documented all our parts on the iGEM Registry of Standard Biological Parts.
  • We investigated the safety issues which could be raised by our project. Take a look at our safety page.

Gold medal

  • We improved the function of the already existing Biobrick BBa_K523013 by extensively characterizing and documenting it. This will help future teams to use it.
  • We prepared an amazing synthetic biology kit which will be used at public events and high school visits at EPFL. Take a look at our Outreach page.
  • We organized a women and engineering's day at our university to promote women in science.


    • European Jamboree Results

    • SILVER MEDAL
    • ADVANCE TO WORLD CHAMPIONSHIP



    Thanks to our sponsors

    Many thanks to our sponsors who made it possible for us to take part in this amazing competition.

    Team-EPF-Lausanne sponso.jpg



Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne"