Team:Cornell/notebook/plasmids

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<h2>Plasmids</h2>
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<h2 class="centered">Plasmids</h2>
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<table class="tableizer-table" rules="all">
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<tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th><th>Antifungal Resistance</th></tr>
<tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th><th>Antifungal Resistance</th></tr>
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  <tr><td>pC13A</td><td>BBa_K523022</td><td>crtEIB</td><td>Kit Plate 1 8P</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
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  <tr><td>pC13A</td><td>BBa_K523022</td><td>crtEBI</td><td>Kit Plate 1 8P</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
  <tr><td>pC13B</td><td>BBa_K539119</td><td>crtY</td><td>Kit Plate 1 12K</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
  <tr><td>pC13B</td><td>BBa_K539119</td><td>crtY</td><td>Kit Plate 1 12K</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
  <tr><td>pA13C</td><td>pIVEX2.3d</td><td>T7 promoter/terminator</td><td>Luo Lab</td><td>pIVEX2.3d</td><td>Ampicillin</td><td>N/A</td></tr>
  <tr><td>pA13C</td><td>pIVEX2.3d</td><td>T7 promoter/terminator</td><td>Luo Lab</td><td>pIVEX2.3d</td><td>Ampicillin</td><td>N/A</td></tr>
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  <tr><td>pC13DR</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI</td><td>DQ, DO</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
  <tr><td>pC13DR</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI</td><td>DQ, DO</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
  <tr><td>pC13DS</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII</td><td>DS, DR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
  <tr><td>pC13DS</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII</td><td>DS, DR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
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`<tr><td>pC13DT</td><td>unsubmitted</td><td>T7 + crtEBI</td><td>A, D</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr>
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<tr><td>pC13DU</td><td>unsubmitted</td><td>Lhom + PtrpC + nptIIBB + Rhom</td><td>BK, BM, Y</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
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<tr><td>pC13DV</td><td>unsubmitted</td><td>Lhom + PgpdA + nptIIBB + Rhom</td><td>BK, BM ,BQ</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr>
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<tr><td>pC13DW</td><td>unsubmitted</td><td>Lhom + PgpdA + hphBB + Rhom</td><td>BK, BM, BR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Hygromycin</td></tr>
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<tr><td>pS13DX</td><td>invalid</td><td>Lhom + PtrpC + nptIIBB + Rhom</td><td>BK, BM, Y</td><td>pOSCAR</td><td>Spectinomycin</td><td>Geneticin</td></tr>
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<tr><td>pS13DY</td><td>invalid</td><td>Lhom + PgpdA + nptIIBB + Rhom</td><td>BK, BM ,BQ</td><td>pOSCAR</td><td>Spectinomycin</td><td>Geneticin</td></tr>
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<tr><td>pS13DZ</td><td>invalid</td><td>Lhom + PgpdA + hphBB + Rhom</td><td>BK, BM, BR</td><td>pOSCAR</td><td>Spectinomycin</td><td>Hygromycin</td>
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<tr><td>pAh13EA</td><td>invalid</td><td>hph + C. heterostrophus Pgpd</td><td>BK, BM, BR</td><td>pHPVEL1</td><td>Spectinomycin</td><td>Hygromycin</td>
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<tr><td>pS13EB</td><td>invalid</td><td>pOSCAR backbone for transformation into agrobacterium</td><td>BK, BM, BR</td><td>pOSCAR</td><td>Spectinomycin</td><td>Hygromycin</td>
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Latest revision as of 03:17, 29 October 2013

Cornell University Genetically Engineered Machines

Plasmids

`
Plasmid NamePartContents/DescriptionOriginBackboneAntibiotic ResistanceAntifungal Resistance
pC13ABBa_K523022crtEBIKit Plate 1 8PpSB1C3ChloramphenicolN/A
pC13BBBa_K539119crtYKit Plate 1 12KpSB1C3ChloramphenicolN/A
pA13CpIVEX2.3dT7 promoter/terminatorLuo LabpIVEX2.3dAmpicillinN/A
pAK13DBBa_I712074T7 promoterKit Plate 5 6NpSB1AK8AmpicillinN/A
pC13EBBa_K145001T7 polymeraseKit Plate 4 2CpSB1C3ChloramphenicolN/A
pC13FBBa_J04450mRFP in pSB1C3T.E. KitpSB1C3ChloramphenicolN/A
pAh13GpUCATPHPtrpC, hph, TtrpCTurgeon labpUCATPHAmpicillinHygromycin
pAg13HpNGPgpd (C. h.), nptIITurgeon labpNGAmpicillinGeneticin
pC13IBBa_K1021000crtEApSB1C3ChloramphenicolN/A
pC13Jinvalidrbs + crtEApSB1C3ChloramphenicolN/A
pC13KBBa_K118003crtIKit Plate 3 2BpSB1C3ChloramphenicolN/A
pC13LBBa_K118005rbs + crtIKit Plate 4 4ApSB1C3ChloramphenicolN/A
pC13MBBa_K118002crtBKit Plate 3 17NpSB1C3ChloramphenicolN/A
pC13NBBa_K118006rbs + crtBKit Plate 3 17PpSB1C3ChloramphenicolN/A
pC13OBBa_I742155rbs + crtYKit Plate 5 17PpSB1C3ChloramphenicolN/A
pC13PBBa_K1021007PtrpCGpSB1C3ChloramphenicolN/A
pC13QBBa_K1021008TtrpCGpSB1C3ChloramphenicolN/A
pCg13RinvalidnptIIHpSB1C3ChloramphenicolGeneticin
pCg13SBBa_K1021001nptIIBBRpSB1C3ChloramphenicolGeneticin
pAb13TpBARGPE1PtrpC, bar, TtrpC, PgpdA, loxFungal Genetics Stock CenterpBARGPE1AmpicillinPhosphonothricin
pCg13UinvalidnptIIBB + TtrpCS, QpSB1C3ChloramphenicolGeneticin
pCh13VinvalidhphG, FpSB1C3ChloramphenicolHygromycin
pCh13WBBa_K1021002hphBBVpSB1C3ChloramphenicolHygromycin
pCp13XBBa_K1021003barT, FpSB1C3ChloramphenicolPhosphonothricin
pCg13YBBa_K1021015PtrpC + nptIIBBP, SpSB1C3ChloramphenicolGeneticin
pC13ZBBa_K082003GFP(+LVA)Kit Plate 3 20NpSB1C3ChloramphenicolN/A
pAK13AAinvalidPT7 + crtED, ApSB1AK8Ampicillin & KanamycinN/A
pAK13ABinvalidPT7 + crtBD, MpSB1AK8Ampicillin & KanamycinN/A
pAK13ACinvalidPT7 + crtID, KpSB1AK8Ampicillin & KanamycinN/A
pAK13ADinvalidPT7 + crtYD, BpSB1AK8Ampicillin & KanamycinN/A
pC13AEBBa_K1021016PT7 + crtEF, AApSB1C3ChloramphenicolN/A
pC13AFBBa_K1021017PT7 + crtBF, ABpSB1C3ChloramphenicolN/A
pC13AGBBa_K1021018PT7 + crtIF, ACpSB1C3ChloramphenicolN/A
pC13AHBBa_K1021019PT7 + crtYF, ADpSB1C3ChloramphenicolN/A
pC13AIBBa_J61046lox siteKit Plate 3 3LpSB1C3ChloramphenicolN/A
pA13AJBBa_J61046lox siteKit Plate 5 24BpSB1A2AmpicillinN/A
pC13AKBBa_E1010mRFP cds onlyKit Plate 3 12NpSB1C3ChloramphenicolN/A
pK13ALBBa_E1010mRFP cds onlyKit Plate 5 18FpSB2K3KanamycinN/A
pA13AMBBa_J23100strongest Anderson constitutive promoter with mRFP downstreamKit Plate 5 18CBBa_J61002Ampicillin & KanamycinN/A
pAK13ANinvalidPT7+GFPD, ZpSB1AK8Ampicillin & KanamycinN/A
pAK13AOinvalidPT7+mRFPD, AK/ALpSB1AK8Ampicillin & KanamycinN/A
pAK13APinvalidPT7+barD, XpSB1AK8Ampicillin & KanamycinPhosphonothricin
pC13AQBBa_K1021020PT7+GFPAN, FpSB1C3ChloramphenicolN/A
pC13ARBBa_K1021021PT7+mRFPAO, FpSB1C3ChloramphenicolN/A
pC13ASBBa_K1021022PT7+barAP, FpSB1C3ChloramphenicolPhosphonothricin
pC13ATBBa_K1021023PtrpC+GFPP, ZpSB1C3ChloramphenicolN/A
pC13AUBBa_K1021024PtrpC+mRFPP, AK/ALpSB1C3ChloramphenicolN/A
pC13AVBBa_K1021025PtrpC+barP, XpSB1C3ChloramphenicolPhosphonothricin
pA13AWinvalidAnderson promoter + T7PolAM, EBBa_J61002AmpicillinN/A
pK13AXinvalidAnderson promoter + T7PolAW, AL (backbone)pSB2K3KanamycinN/A
pC13AYunsubmittedPtrpC+T7PolP, EpSB1C3ChloramphenicolN/A
pC13AZBBa_K1021026Anderson promoter + T7PolAW, FpSB1C3ChloramphenicolN/A
pC13BABBa_K1021004afp1G-blockpSB1C3ChloramphenicolN/A
pAK13BBinvalidPT7+nptIIBBD, SpSB1AK8Ampicillin & KanamycinGeneticin
pC13BCBBa_K1021032PT7+nptIIBBBB, FpSB1C3ChloramphenicolGeneticin
pC13BDunsubmittedPtrpC+crtIP, KpSB1C3ChloramphenicolN/A
pA13BEpHNU3pelAPpelA, flanked by KpnI/BamHITurgeon labpHPGAmpicillinN/A
pC13BFBBa_K1021009PpelABE, FpSB1C3ChloramphenicolN/A
pC13BGBBa_K1021010A. nidulans PgpdAT, FpSB1C3ChloramphenicolN/A
pC13BHinvalidG. lucidum PgpdAGanoderma genome, FpSB1C3ChloramphenicolN/A
pC13BIunsubmittedG. lucidum PgpdA (BB)BHpSB1C3ChloramphenicolN/A
pC13BJBBa_K1021011100bp left region of homology (Ganoderma gpd gene)Ganoderma genome, FpSB1C3ChloramphenicolN/A
pC13BKBBa_K1021012200bp left region of homology (Ganoderma gpd gene)Ganoderma genome, FpSB1C3ChloramphenicolN/A
pC13BLBBa_K1021013100bp right region of homology (Ganoderma gpd gene)Ganoderma genome, FpSB1C3ChloramphenicolN/A
pC13BMBBa_K1021014200bp right region of homology (Ganoderma gpd gene)Ganoderma genome, FpSB1C3ChloramphenicolN/A
pC13BNunsubmittedlox [cutsite 1] [cutsite 2] loxAIpSB1C3ChloramphenicolN/A
pC13BOabandonedshort homology regions flanking two unique cutsitesBJ, BLpSB1C3ChloramphenicolN/A
pC13BPabandonedlong homology regions flanking two unique cutsitesBK, BMpSB1C3ChloramphenicolN/A
pC13BQBBa_K1021027A. nidulans PgpdA + nptIIBBBG, SpSB1C3ChloramphenicolGeneticin
pC13BRBBa_K1021028A. nidulans PgpdA + hphBBBG, WpSB1C3ChloramphenicolHygromycin
pC13BSunsubmittedG. lucidum PgpdABB + nptIIBBBI, SpSB1C3ChloramphenicolGeneticin
pC13BTunsubmittedG. lucidum PgpdABB + hphBBBI, WpSB1C3ChloramphenicolHygromycin
pAK13BUinvalidPT7 + hphBBD, WpSB1AK8Ampicillin & KanamycinHygromycin
pC13BVBBa_K1021033PT7 + hphBBBU, FpSB1C3ChloramphenicolHygromycin
pA13BWpBS185CMV-CrepBS185 CMV CreAddgenepBS185AmpicillinN/A
pC13BXBBa_K1021005Cre recombinaseBWpSB1C3ChloramphenicolN/A
pC13BYunsubmittedPpelA + CreBF, BXpSB1C3ChloramphenicolN/A
pC13BZunsubmittedPpelA + GFPBF, ZpSB1C3ChloramphenicolN/A
pC13CAunsubmittedPpelA + mRFPBF, ALpSB1C3ChloramphenicolN/A
pC13CBunsubmittedPT7 + GFP + PtrpC + nptIIBBY, AQpSB1C3ChloramphenicolGeneticin
pC13CCBBa_K1021029PtrpC + nptIIBB + TtrpCY, QpSB1C3ChloramphenicolGeneticin
pC13CDBBa_K1021030PtrpC + hphBBP, WpSB1C3ChloramphenicolHygromycin
pC13CEBBa_K1021034PtrpC + hphBB +TtrpCCD, QpSB1C3ChloramphenicolHygromycin
pC13CFunsubmittedA. n. PgpdA + GFPBG, ZpSB1C3ChloramphenicolN/A
pC13CGBBa_K1021031A. n. PgpdA + mRFPBG, AK/ALpSB1C3ChloramphenicolN/A
pC13CHunsubmittedG. l. PgpdA + GFPBH, ZpSB1C3ChloramphenicolN/A
pC13CIunsubmittedG. I. PgpdA + mRFPBH, AK/ALpSB1C3ChloramphenicolN/A
pC13CJBBa_I742111Limonene synthaseKit plate 4pSB1C3ChloramphenicolN/A
pAK13CKunsubmittedPT7+Limonene SynthaseCJ, DpSB1AK8Ampicillin & KanamycinN/A
pC13CLBBa_K1021006Cht1_2 in pSB1C3IDT GeneblockpSB1C3ChloramphenicolN/A
pC13CMunsubmittedPpelA + Cht1_2CL, BFpSB1C3ChloramphenicolN/A
pS13CNBBa_K112306Lambda HolinKit plate 3 2012 BBa_K112306BBa_K112950SpectinomycinN/A
pC13COunsubmittedLambda Holin in pSB1C3CNpSB1C3ChloramphenicolN/A
pC13CPunsubmittedPpelA+Lambda HolinCOpSB1C3ChloramphenicolN/A
pAK13CQinvalidPT7 + rbs + crtED, JpSB1AK8Ampicillin & KanamycinN/A
pAK13CRinvalidPT7 + rbs + crtBD, NpSB1AK8Ampicillin & KanamycinN/A
pAK13CSinvalidPT7 + rbs + crtID, LpSB1AK8Ampicillin & KanamycinN/A
pAK13CTinvalidPT7 + rbs + crtYD, OpSB1AK8Ampicillin & KanamycinN/A
pC13CUunsubmittedPT7 + rbs + crtEF, CQpSB1C3ChloramphenicolN/A
pC13CVunsubmittedPT7 + rbs + crtBF, CRpSB1C3ChloramphenicolN/A
pC13CWunsubmittedPT7 + rbs + crtIF, CSpSB1C3ChloramphenicolN/A
pC13CXunsubmittedPT7 + rbs + crtYF, CTpSB1C3ChloramphenicolN/A
pAK13CYinvalidPT7 + rbs + GFPD, DBpSB1AK8Ampicillin & KanamycinN/A
pA13CZBBa_B0034RBS (strong)Kit Plate 5, Well 2MpSB1A2AmpicillinN/A
pAK13DAinvalidPT7 + rbs + mRFPD, DCpSB1AK8Ampicillin & KanamycinN/A
pA13DBinvalidRBS+GFPCZ, ZpSB1A2AmpicillinN/A
pA13DCinvalidRBS+mRFPCZ, AKpSB1A2AmpicillinN/A
pC13DDunsubmittedPT7+Limonene SynthaseF, CKpSB1C3ChloramphenicolN/A
pC13DEunsubmittedlox-PtrpC-nptIIBB-loxY, BNpSB1C3ChloramphenicolGeneticin
pC13DFunsubmittedlox-A.PgpdA-nptIIBB-loxBN, BQpSB1C3ChloramphenicolGeneticin
pC13DGunsubmittedlox-A.PgpdA-hphBB-loxBN, BRpSB1C3ChloramphenicolHygromycin
pC13DHunsubmittedlox-PtrpC-hphBB-loxBN, CDpSB1C3ChloramphenicolHygromycin
pC13DIunsubmittedPtrpC + hphBB + GFPCD, ZpSB1C3ChloramphenicolHygromycin
pC13DJunsubmittedPT7 + crtB + PT7 + crtIAF, AGpSB1C3ChloramphenicolN/A
pC13DKunsubmittedPT7 + crtE + PT7 + crtB + PT7 + crtIDJ, AEpSB1C3ChloramphenicolN/A
pC13DLunsubmittedPT7 + crtE + PT7 + crtB + PT7 + crtI + PT7 + nptIIBBDK, BCpSB1C3ChloramphenicolGeneticin
pC13DMunsubmittedPT7 + rbs + crtB + PT7 + rbs + crtICV, CWpSB1C3ChloramphenicolN/A
pC13DNunsubmittedPT7 + rbs + crtE + PT7 + rbs + crtB + PT7 + rbs + crtIDM, CUpSB1C3ChloramphenicolN/A
pC13DOunsubmittedGan. PgpdA + crtEBI, IpSB1C3ChloramphenicolN/A
pC13DPunsubmittedAsp. PgpdA + crtBBG, MpSB1C3ChloramphenicolN/A
pC13DQunsubmittedAsp. PgpdA + crtB + PtrpC + crtIDP, BDpSB1C3ChloramphenicolN/A
pC13DRunsubmittedGan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtIDQ, DOpSB1C3ChloramphenicolN/A
pC13DSunsubmittedGan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptIIDS, DRpSB1C3ChloramphenicolGeneticin
pC13DTunsubmittedT7 + crtEBIA, DpSB1C3ChloramphenicolN/A
pC13DUunsubmittedLhom + PtrpC + nptIIBB + RhomBK, BM, YpSB1C3ChloramphenicolGeneticin
pC13DVunsubmittedLhom + PgpdA + nptIIBB + RhomBK, BM ,BQpSB1C3ChloramphenicolGeneticin
pC13DWunsubmittedLhom + PgpdA + hphBB + RhomBK, BM, BRpSB1C3ChloramphenicolHygromycin
pS13DXinvalidLhom + PtrpC + nptIIBB + RhomBK, BM, YpOSCARSpectinomycinGeneticin
pS13DYinvalidLhom + PgpdA + nptIIBB + RhomBK, BM ,BQpOSCARSpectinomycinGeneticin
pS13DZinvalidLhom + PgpdA + hphBB + RhomBK, BM, BRpOSCARSpectinomycinHygromycin
pAh13EAinvalidhph + C. heterostrophus PgpdBK, BM, BRpHPVEL1SpectinomycinHygromycin
pS13EBinvalidpOSCAR backbone for transformation into agrobacteriumBK, BM, BRpOSCARSpectinomycinHygromycin

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