Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog

From 2013.igem.org

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JL, LP
JL, LP
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* Performed amplification and purification of insert as part of [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|Cloning T7 Capsid Protein into iGEM Registry]].
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* Performed amplification and purification of insert as part of [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]].
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JL, LP
JL, LP
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* Ran a low melt gel for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|Cloning T7 Capsid Protein into iGEM Registry]].
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* Ran a low melt gel for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]].
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<font size="4"> '''10/8/13''' </font>
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<font size="4"> '''10/20/13''' </font>
JL
JL
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* Started [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]] by propagating the mutants.
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* Performed the repeat PCR and gel step in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
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<font size="4"> '''10/9/13''' </font>
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<font size="4"> '''10/21/13''' </font>
JL, LP
JL, LP
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* Purified the propagated mutant from [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Did restriction digest for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
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* Started approximately 20 mL of E coli B liquid culture overnight.
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<font size="4"> '''10/10/13''' </font>
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<font size="4"> '''10/22/13''' </font>
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LP
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JL
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* Performed spot tests to estimate the mutant phage titer in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Low-melt gel for purification of sticky-ended insert/vector for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
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* Started approximately 20 mL of E coli B liquid culture overnight.
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JL
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<font size="4"> '''10/23/13''' </font>
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* Designed primers for cloning the major and minor capsid proteins into the iGEM registry: BI309 (Xbal) and BI310 (SpeI)
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JL, LP
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* Did ligation and transformation for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
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<font size="4"> '''10/11/13''' </font>
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<font size="4"> '''10/24/13''' </font>
JL, LP
JL, LP
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* Plated the mutant phage at -7 to verify their phenotypic stability. For specifics, please see [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Did Colony PCR and Overnights for Vector Purification for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]]
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* Designed primers for more accurate sequencing: BI319 (160bp upstream from start codon) and BI320 (-160 downstream from minor capsid protein stop codon)
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<font size="4"> '''10/12/13''' </font>
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<font size="4"> '''10/25/13''' </font>
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JL
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JL, LP
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* Started approximately 15mL of E coli B liquid culture overnight
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* Did plasmid cleanup for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.18 Cloning T7 Capsid Protein into iGEM Registry|10.18 Cloning T7 Capsid Protein into iGEM Registry]] and sent the plasmid into iGEM!
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<font size="4"> '''10/13/13''' </font>
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<font size="4"> '''10/27/13''' </font>
JL
JL
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* Plated more phage from CsCl gradient to select for mutant. Specifics are recorded in [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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<font size="4"> '''9/27/13''' </font>
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<font size="4"> '''10/28/13''' </font>
JL, LP
JL, LP
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* Determined results for [[Team:BYU_Provo/Notebook/SmallPhage/Summerexp/9.13_Mutagen_Concentration_Test|9.13 Mutagen Concentration Test - Ninth Protocol]].
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* Propagated WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]]
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* Finished updating the wiki.
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* Started PCR reaction for sequencing WT, S4, S10, S21, S22, and L8 for [[Team:BYU_Provo/Notebook/SmallPhage/Fallexp/10.8 Characterization of Mutant Phage|10.8 Characterization of Mutant Phage]].
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* Started approximately 30mL of E. coli B overnight.
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* Mapped out mutation for S4 capsid protein.
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* Finished the wiki.
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Latest revision as of 03:25, 29 October 2013


Small Phage September - October Notebook: October 16 - October 31 Daily Log



Small Phage
March-April
May-June
July-August
September-October

10/16/13

JL, LP


10/18/13

JL, LP


10/19/13

JL, LP


10/20/13

JL


10/21/13

JL, LP


10/22/13

JL


10/23/13

JL, LP


10/24/13

JL, LP


10/25/13

JL, LP


10/27/13

JL


10/28/13

JL, LP

  • Mapped out mutation for S4 capsid protein.
  • Finished the wiki.



Retrieved from "http://2013.igem.org/Team:BYU_Provo/Notebook/SmallPhage/Fallexp/Period4/Dailylog"