Team:BYU Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog

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Latest revision as of 20:00, 27 September 2013

Large Phage July - August Notebook: September 16 - September 27 Daily Log

Large Phage

9/16/13

Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight.

9/18/13

We looked at the results from the spot test today. We found that: All fractions had phage at 10^0 Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques. Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.

We also measured the capsids and tails of the TEM images.

9/23/13

We spent our time in class and over the weekend measuring the phage capsids and tails to see if there is a correlation between their sizes before and after our mutagenesis.

9/25/13

-Today we worked on the project poster image for the phage diagram

@@ Line 1: / Line 1: @@
 {{TeamBYUProvo}}
-==July==
+<br>
+{| width="100%"
+| colspan="3" | <font color="#333399" size="5" font face="Calibri">
-===7/17/13===
+: '''Large Phage July - August Notebook: September 16 - September 27 Daily Log'''</font>
-Today I did another round of mutagenesis using the lysis inhibition technique.  I did two flasks.  The first flask had 20 ug/mL of adenine which was added with the bacteria and 25 mL of LB broth; the second flask had 40 ug/mL of adenine added with the bacteria and 25 mL of LB broth.  Both flasks incubated at 37C on the shaker for 3.5 hrs until slightly turbid.  I then added 200 ug/mL of bromodeoxyuridine, 20 ug/mL of uracil, and .5 mL of phage to flask one; I added 400 ug/mL of bromodeoxyuridine, 40 ug/mL of uracil, and .5 mL of phage to flask two.  They incubated in the same location for 3 hours.
-===7/19/13===
+<br>
+<br>
-===7/22/13===
+|- valign="top"
+| style="width: 22%; background-color: transparent;"|
-===7/24/13===
+<font color="#333399" size="3" font face="Calibri">
-===7/26/13===
+<font size = "4">
-===7/29/13===
+: <u> '''Large Phage''' </u> </font>
-===7/31/13===
+: [[Team:BYU Provo/Notebook/LargePhage/Winterexp|March-April]]
+: [[Team:BYU Provo/Notebook/LargePhage/Springexp|May-June]]
+: [[Team:BYU Provo/Notebook/LargePhage/Summerexp|July-August]]
+: [[Team:BYU Provo/Notebook/LargePhage/Fallexp|September-October]]
+</font>
+| style="width: 82%; background-color: transparent;"|
+<font face="Calibri" size="3">
+<font size="4"> '''9/16/13''' </font>
+Today Jade and LJ helped us spot test all of the fractions above the band from our last CsCl gradient. These fractions were numbered 12-19, but 15 was skipped. Each fraction was spot tested at a titer of 10^0, 10^-2, and 10^-4 and incubated agar side down at 37C overnight.
+<br>
+<font size="4"> '''9/18/13''' </font>
+We looked at the results from the spot test today. We found that:
+All fractions had phage at 10^0
+Fractions 18, 19, 17, 16, and 14 had large clearings still at 10^-2 while 13 and 12 just had individual plaques.
+Fractions 18, 19, 17, 16, and 14 had individual plaques at 10^-4.
+We also measured the capsids and tails of the TEM images.
+[[File:September18-1.jpg|400px]]
+[[File:September18-2.jpg|400px]]
+[[File:September18-3.jpg|400px]]
+<br>
+<font size="4"> '''9/23/13''' </font>
+We spent our time in class and over the weekend measuring the phage capsids and tails to see if there is a correlation between their sizes before and after our mutagenesis.
+<br>
+<font size="4"> '''9/25/13''' </font>
+-Today we worked on the project poster image for the phage diagram
+<br>
 {{TeamBYUProvoFooter}}